On the adsorption of IgG onto polystyrene particles: electrophoretic mobility and critical coagulation concentration

Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal‐Based Rapid Diagnostic

“…These two contributions are not independent; that is, a higher flexibility in the protein structure enables a more efficient coverage of the sorbent surface by the protein (90). It was suggested that if the incubation time was sufficient for the occurrence of the structure alteration of protein molecule, it does not readily desorb by latex dilution or changing pH medium (88,91).…”

“…According to the former works, it was reported that the maximum G ads value of various proteins onto the particle surface is obtained when the medium pH coincides with pI of the protein (38,88,91,94). At pH equals to pI, the minimal lateral interaction between neighboring adsorbed protein molecules renders the maximum G ads value.…”

“…A dramatic reduction in the value of G ads with increasing ionic strength is expected under the condition of electrostatic attraction between the opposite charges of protein and sorbent due to the charge screening effect. On the contrary, for the adsorption system in which electrostatic repulsion exists between the same charge of protein and latex particle, the increase in ionic strength would increase the G ads value (91,94). It was noticed that, for the sorbent having hydrophilic layer on its surface (hairy particle), the increase of the ionic strength led to a shrinkage of the interfacial (93) hydrophilic layer (87,95), and accordingly the reduction in the accessible surface for protein adsorption (87).…”

Revealing deposition mechanism of colloid particles on human serum albumin monolayers

“…Analogously, controlled adsorption of albumins is applied for blocking colloid particle surface in order to efficiently perform various agglutination immunoassays [2,3].…”

“…The kinetics of polystyrene particle deposition on HSA monolayers of various coverage determined by optical microscope imaging for ionic strength 0.15 M, pH 3.5, bulk particle concentration 0.5% (5,000 mg L -1 ). The points denote the experimental data obtained for N a = 5x103 [µm -2 ] (•), N a = 3x10 2 [µm -2 ] (), N a = 60 [µm -2 ] (○). The solid lines 1-3 show the theoretical results pertinent to adsorption calculated using the RSSA model.…”