On the active transport of organic acids (fluorescein) in the choroid plexus of the rabbit

Бреслер, С. Е.; Bresler, V. M.; Kazbekov, É. N.; Nikiforov, A. A.; Vasilieva, N. N.

doi:10.1016/0005-2736(79)90119-6

Cited by 27 publications

(15 citation statements)

References 13 publications

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“…Using fluorescenece microscopy, Bresler et al (10) have shown that fluorescein is concentrated by the isolate(d rabbit choroid plexus by a mechanism very similar to what we have shown for riboflavin as discussed above. The KT for fluorescein accumulation by the choroid plexus is -40 ,uM (10).…”

Section: Resultssupporting

confidence: 79%

“…The KT for fluorescein accumulation by the choroid plexus is -40 ,uM (10). In Table I, the 'C50 for fluioresceiin is between 10 and 100 ,mM.…”

Section: Resultsmentioning

confidence: 96%

“…If blood were flowing through the choroid plexus, presumably the riboflavin would be transported out of the choroid plextus by the blood. Bresler et al (10) have published fluorescence photomierographs of fluorescein tranisport by isolated rabbit choroidl plexus in vitro. As with riboflavin, fluorescein is trainsferred from the mediium into the extracellular space of the choroid plexus via the choroid plexus epithelial cells (10).…”

Section: Resultsmentioning

confidence: 99%

“…Bresler et al (10) have published fluorescence photomierographs of fluorescein tranisport by isolated rabbit choroidl plexus in vitro. As with riboflavin, fluorescein is trainsferred from the mediium into the extracellular space of the choroid plexus via the choroid plexus epithelial cells (10). As with riboflavin, fluioreseein did not enter the erythrocytes within the choroid plexus or the nuclei of the choroid plexus epithelial cells (10).…”

Section: Resultsmentioning

confidence: 99%

“…To study the uptake of riboflavin by the isolated rabbit choroid plexus, advantage was taken of the marked fluorescence of riboflavin and methodology recently described by Bresler et al (10). Briefly, fluorescence microscopy was performed with a Leitz-Wetzlar Orthoplan Universal Large Field Microscope with a xenon lamp for fluorescence (E. Leitz, Inc., Rockleigh, N.…”

Section: Niethodsmentioning

confidence: 99%

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Riboflavin transport in the central nervous system. Characterization and effects of drugs.

Spector¹

1980

J. Clin. Invest.

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A B S T R A c 1 The relationship of riboflavin transport to the tranisport ofother suI)stances includinig drugs in ral)bit choroid plexus, the anaiatomical locus of the blood-cerebrospinal fluid barrier, and brain cells were stuidied in vivo and( in vitro. In vitro, the ability of rabbit choroi(d plexus to trainsport riboflavin from the imediumil (cerebrospinal fluid surface) through the choroid plexus epithelial cells into the extracellular anid vaseculatr spaces of the choroid plexus was docuimented using fluoreseence microscopy.

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Section: Resultssupporting

confidence: 79%

“…The KT for fluorescein accumulation by the choroid plexus is -40 ,uM (10). In Table I, the 'C50 for fluioresceiin is between 10 and 100 ,mM.…”

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Niethodsmentioning

confidence: 99%

See 3 more Smart Citations

Riboflavin transport in the central nervous system. Characterization and effects of drugs.

Spector¹

1980

J. Clin. Invest.

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Porcinechoroid plexus epithelial cells in culture: Regulation of barrier properties and transport processes

Haselbach

Wegener

Decker

et al. 2000

Microsc. Res. Tech.

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The epithelial cells of the choroid plexus are the structural basis of the blood‐cerebrospinal fluid (CSF)‐barrier. Here we summarise our recent efforts to culture those cells mainly on permeable supports in vitro. Isolated from porcine brains, we report a simple protocol for the primary culture using cytosine arabinoside as an additive that is cytotoxic for other cells except the plexus epithelial cells. Enhanced barrier properties are obtained by withdrawal of serum from the culture medium after confluency is reached. Cells improve their polarity, permeability for hydrophilic substrates is lowered, electrical resistance is increased tenfold, and a pH‐gradient is built up across the cell monolayer. Polarised secretion of proteins and most importantly fluid secretion into the apical filter compartment was attained and proven to be dependent on the Na+,K+‐ATPase activity. Active transport processes (penicillin G, riboflavin, myo‐inositol, ascorbic acid) were studied and clearly showed the involvement of the organic anion transporter. The permeability of the barrier was found to be regulated by cyclic adenosine monophosphate (cAMP). Moreover, we report that cell proliferation and differentiation is controlled by components of the extracellular matrix. The present culture model could now be used as an in vitro system to quantify drug transport across the blood‐CSF‐barrier. Microsc. Res. Tech. 52:137–152, 2001. © 2001 Wiley‐Liss, Inc.

show abstract

Porcine choroid plexus epithelial cells in culture: Regulation of barrier properties and transport processes

Haselbach¹,

Wegener²,

Decker³

et al. 2001

Microsc. Res. Tech.

View full text Add to dashboard Cite

The epithelial cells of the choroid plexus are the structural basis of the blood-cerebrospinal fluid (CSF)-barrier. Here we summarise our recent efforts to culture those cells mainly on permeable supports in vitro. Isolated from porcine brains, we report a simple protocol for the primary culture using cytosine arabinoside as an additive that is cytotoxic for other cells except the plexus epithelial cells. Enhanced barrier properties are obtained by withdrawal of serum from the culture medium after confluency is reached. Cells improve their polarity, permeability for hydrophilic substrates is lowered, electrical resistance is increased tenfold, and a pH-gradient is built up across the cell monolayer. Polarised secretion of proteins and most importantly fluid secretion into the apical filter compartment was attained and proven to be dependent on the Na(+),K(+)-ATPase activity. Active transport processes (penicillin G, riboflavin, myo-inositol, ascorbic acid) were studied and clearly showed the involvement of the organic anion transporter. The permeability of the barrier was found to be regulated by cyclic adenosine monophosphate (cAMP). Moreover, we report that cell proliferation and differentiation is controlled by components of the extracellular matrix. The present culture model could now be used as an in vitro system to quantify drug transport across the blood-CSF-barrier.

show abstract

On the active transport of organic acids (fluorescein) in the choroid plexus of the rabbit

Cited by 27 publications

References 13 publications

Riboflavin transport in the central nervous system. Characterization and effects of drugs.

Riboflavin transport in the central nervous system. Characterization and effects of drugs.

Porcinechoroid plexus epithelial cells in culture: Regulation of barrier properties and transport processes

Porcine choroid plexus epithelial cells in culture: Regulation of barrier properties and transport processes

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