On the Acoustic Properties of Vaporized Submicron Perfluorocarbon Droplets

Reznik, Nikita; Lajoinie, Guillaume; Shpak, Oleksandr; Gelderblom, Erik; Williams, Ronald J.; Jong, Nico de; Versluis, Michel; Burns, Peter N.

doi:10.1016/j.ultrasmedbio.2013.11.025

Cited by 38 publications

(29 citation statements)

References 33 publications

(37 reference statements)

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“…This implies that the surfactant initially stabilizing the droplet can also stabilize the bubble and support nonlinear oscillations. 131 Perfluorocarbon droplets also offer the potential to be used as multimodality agents, since they can be activated using ultrasound 127,130,131 but also using light when internally loaded with plasmonic nanoparticles, 132 which leads to an increase in photoacoustic contrast and the creation of a ultrasound microbubble contrast agent simultaneously.…”

Section: B Droplets As Precursorsmentioning

confidence: 99%

“…159 Vaporization of liquid precursors of bubble also falls in this category. 131 This regime is the most reported one in the study of bubble-cell interactions, for example: Smith et al reported sonoporation resulting from cavitation of echogenic liposomes, 123 Zhou et al used inertial cavitation to porate oocyte cells, 23 and Zhao et al used cavitation to induce cell apoptosis. 11 Using larger bubbles, Ohl et al observed two regimes of viable porated cell and detached dead cells.…”

Section: Acoustic Excitationmentioning

confidence: 99%

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In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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Besides their use as contrast agents for ultrasound imaging, microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes. V C 2016 AIP Publishing LLC.

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Section: B Droplets As Precursorsmentioning

confidence: 99%

Section: Acoustic Excitationmentioning

confidence: 99%

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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“…16 This leads to a rapid increase in core volume resulting in the production of microbubble contrast agents having contrast properties similar to those currently used for diagnostic applications. 17,18 Moreover, the mechanical perturbations produced during the ADV process could induce bioeffects on nearby cells and enhance localized drug delivery. 19 To date, only few studies have investigated the bioeffects related to ADV.…”

Section: Introductionmentioning

confidence: 99%

Wideband acoustic activation and detection of droplet vaporization events using a capacitive micromachined ultrasonic transducer

Novell¹,

Arena²,

Oralkan³

et al. 2016

The Journal of the Acoustical Society of America

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An ongoing challenge exists in understanding and optimizing the acoustic droplet vaporization (ADV) process to enhance contrast agent effectiveness for biomedical applications. Acoustic signatures from vaporization events can be identified and differentiated from microbubble or tissue signals based on their frequency content. The present study exploited the wide bandwidth of a 128-element capacitive micromachined ultrasonic transducer (CMUT) array for activation (8 MHz) and real-time imaging (1 MHz) of ADV events from droplets circulating in a tube. Compared to a commercial piezoelectric probe, the CMUT array provides a substantial increase of the contrast-to-noise ratio.

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“…Interestingly, the bubbles generated from superheated droplets exhibit characteristics similar to those of phospholipid-coated bubbles. This implies that the surfactant initially stabilizing the droplet can also stabilize the bubble and support non-linear oscillations 117 . Perfluorocarbon droplets also offer the potential to be used as multi-modality agents since they can be activated using ultrasound 114,116,117 but also using light when internally loaded with plasmonic nanoparticles 118 , which leads to an increase in photoacoustic contrast and the creation of a ultrasound microbubble contrast agent simultaneously.…”

Section: Droplets As Precursorsmentioning

confidence: 99%

“…The corresponding acoustic emissions are broadband by nature 138 . Vaporization of liquid precursors of bubble also falls in this category 117 . This regime is the most reported in the study of bubble-cells interactions, for example: Smith et al reported sonoporation resulting from cavitation of echogenic liposomes 111 , Zhou et al used inertial cavitation to porate oocyte cells 18 , and Zhao et al used cavitation to provoke cell apoptosis 11 .…”

Section: Acoustic Excitationmentioning

confidence: 99%

Ultrasound contrast agents: bubbles drops and particles

Lajoinie¹

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The research described in this thesis is part of the research program NanoNextNL, a micro and nanotechnology consortium of the Government of the Netherlands and 130 partners. This thesis was carried out at the Physics of Fluids group of the Faculty of Science and Technology of the University of Twente. Cover design:As a representation of a crucial goal of this work, the cover depicts a confocal microscopy image showing cells selectively porated by loaded microbubbles upon ultrasound exposure. The porated cells take up a blue fluorescent probe. By Ine Lentacker, Ine De Cock and Guillaume Lajoinie. Guide through the thesisIn this thesis, we investigate different types of innovative agents, designed for biomedical use in a context of molecular imaging. In Chapter 1, we review the agents that arouse the interest of the scientific community, most of them at the research stage of development, together with the experimental methods that can be used to study their interaction with cells. Such in vitro investigations are necessary to test on short scales the impact of the various agents on biological structures.We separate the agents in three main categories. The first group consists of stabilized microbubbles, usually by means of phospholipids that fill up the interfacial area between the gas core and the surrounding liquid (water). Such bubbles are clinically used for 40 years as contrast agents for ultrasound owing to their unique resonance behavior. When irradiated with an ultrasound pulse, a bubble experiences volumetric oscillations, that reemit an ultrasound wave back to the transducer. This process makes them unchallenged ultrasound scatterers. The role of these stable microbubbles in new biomedical research directions is discussed in Chapters 2 to 9.Phospholipid coatings were developed in the last decade to do much more than just prevent bubble dissolution. They are now a crucial tool to enrich the microbubbles with specific markers or drug payloads. In Chapter 2, we investigate the behavior of this coating when the microbubbles are subjected to an ultrasound field. We show that the coating is shed away from the microbubble, which is of major interest for molecular imaging combined with drug delivery using microbubbles. We pursue the idea in Chapter 3 where we investigate how a bubble payload, here liposomes labeled with a fluorescent dye, is released under ultrasound exposure. In Chapter 4, we link these direct observations of shedding of the previous Chapters to the subsequent dissolution of the microbubbles, no longer protected by a phospholipid shell. This dissolution, visible in the ultrasound scatter spectrum can be used to remotely quantify the effect of the microbubbles. In support of these observations, we dedicate the next Chapter (Chapter 5) to physically understand the mechanisms underlying the behavior of the phospholipid coating. The application of these findings in practice requires the production a sufficiently high production rate and with great control of such microbubbles. In Chapter 6, we th...

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On the Acoustic Properties of Vaporized Submicron Perfluorocarbon Droplets

Cited by 38 publications

References 33 publications

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

Wideband acoustic activation and detection of droplet vaporization events using a capacitive micromachined ultrasonic transducer

Ultrasound contrast agents: bubbles drops and particles

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