On the Ability of Taphrina deformans to Produce Indoleacetic Acid from Tryptophan by Way of Tryptamine

Abstract

NSunnary. 'I'lTe metalolismii of tryptophan by Taphrina deformnzn% has beell sttudie(d to confirimi the reported ability of this organism ti prod(luce trpl)tanine. Stich amine production was uot observed, (lespite tise of amille oxi(lase inhibitors at levels which should have resulted in the acculmiulationl of tryptamvine in the mie(liuimii. It has been ishown that the metabolites of tryptopbhln includ(e indolepvr-rnic aci(l, in(lolelactic acid, tryptophol, and indoleacetic acid. and that the original report of trypltamiline production muist be reevaltlated in light of the extraction l)rocedlures emiployed.WNhile miuch in(lirect evidence, stiimimiiarize(I in the reviews of Stowe (10)

Biosynthesis of Indoleacetic Acid from Tryptophan-14C in Cell-free Extracts of Pea Shoot Tips

“…The occurrence of IAA in infinitesimal concentrations in most hi'gher plant tissuies, the relaitively small yields obtained from in vitro biosynthesis, and the lability of the compotund in liight, 02 atmosphere and strong acid soluitionis pose = , problems that have never been solved entirely 8 9 10 satisfactorily. Problems in identification and analysis of IAA continue to exist despite the development of many valuable proce(lures involving paper B (2,5,7,12,13,15,23,33,34), thin-layer (17,21,22) and partition column (15,24,25,26) chromatography; electrophoresis (15, 23); gas chromatography (26); s9pectrophotofltiorometry (26); colorimetry (2,5,7,12,13,15,23,24,26,33,34) and spectrophotometry (7).…”

Auxin and Plant-Microbe Interactions

“…In Bacillus cereus, tryptophan decarboxylase activity could be identified (Perley and Stowe 1966), and in Azospirillum exogenously supplied tryptamine is converted to IAA (Hartmann et al 1983).…”

Salkowski’s Reagent Test as a Primary Screening Index for Functionalities of Rhizobacteria Isolated from Wild Dipterocarp Saplings Growing Naturally on Medium-Strongly Acidic Tropical Peat Soil

“…As exogenous IAA in the rhizosphere is a key phytohormone-like chemical substance for rootmicrobe interaction, the bio-conversion process of TRP to IAA in bacteria has been investigated well following Perley and Stowe. 10) Particularly, TRP transaminase, which catalyzes an oxidative deamination on TRP to yield indole-3-pyruvic acid (IPyA), was first reported in Enterobacter cloacae, which showed a remarkable capability of IAA production. 11,12) Hence Salkowski's reagent test, specified for the detection of IAA and its precursory compound IPyA, is often used for primary screening of IAA-producing rhizobacteria.…”