On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

Sofi, Lateef Ahmad; Sherwani, Rana K; Hasan, Mehboob; Nobin, Hage

doi:10.1159/000354027

Cited by 4 publications

(7 citation statements)

References 6 publications

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“…In our study, BCB was the least favored of all the smears as the background staining was too bright and smudgy, the cell morphology was not clear, with smudgy staining of the nucleus and cytoplasm. However, the study by Sofi et al showed BCB to give good cytoplasmic and nuclear details which is in contrast to our finding. This may be due to the fact that we have done a comparative evaluation of four rapid stains.…”

Section: Discussioncontrasting

confidence: 99%

“…However, the study by Sofi et al 9 showed BCB to give good cytoplasmic and nuclear details which is in contrast to our finding. This may be due to the fact that we have done a comparative evaluation of four rapid stains.…”

Section: Whocontrasting

confidence: 99%

“…There are only a few studies that compare different rapid staining techniques. The different rapid stains for on‐site evaluation previously described are diff quik, toluidine blue (TB), brilliant cresyl blue (BCB), ultra‐fast pap stains, and rapid H&E . Which among these stains is the quickest/most cost‐effective/best in staining quality?…”

Section: Introductionmentioning

confidence: 99%

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Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

Agarwal

Toi

Subramaniam

et al. 2018

Diagnostic Cytopathology

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Objective Rapid on‐site evaluation (ROSE) is a technique beneficial in determining the adequacy of the samples, thereby increasing the diagnostic yield, useful in triage of specimens for ancillary studies and can also help determine a preliminary diagnosis in emergency cases. The different rapid stains for on‐site evaluation described in the literature are diff quik, toluidine blue (TB), brilliant cresyl blue (BCB), ultra‐fast Pap stains, and rapid hematoxylin and eosin (H&E). This study was undertaken as there is sparse literature regarding the best and the most cost‐effective rapid stain. Method Fine needle aspiration samples from 200 patients with palpable swellings in easily accessible regions were taken. Smears stained by rapid and routine stains were assessed based on four parameters, with provisional diagnosis on the rapid stained smears. A comparative analysis of the advantages and disadvantages of the rapid stains was carried out with appropriate statistical tests with the routinely stained smears as gold standard. Results There was adequate material in 100% of ROSE smears. rapid pap stained smears showed well preserved cytoplasmic details, nuclear details, and background details. The time taken was least with TB and BCB being 5 s each. The most cost‐effective was found to be TB. Conclusions We conclude that TB is the most cost‐effective, quick, least labor‐intensive, and reliable rapid stain for ROSE especially in resource‐poor settings.

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Section: Discussioncontrasting

confidence: 99%

Section: Whocontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

Agarwal

Toi

Subramaniam

et al. 2018

Diagnostic Cytopathology

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“…Results obtained in our study were similar to the study done by Lateef et al 10 They were also used Brilliant cresyl blue for rapid staining. They assess the comparative diagnostic efficacy of brilliant cresyl blue stain with Pap stain.…”

Section: Comparison Of Diagnostic Accuracysupporting

confidence: 91%

Utility of rapid brillant cresyl blue stained wet mount preparation in routine fine needle aspiration cytology

Vipina¹,

Preethamol²,

Anitha³

2020

ACHR

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Fine Needle Aspiration Cytology is the preliminary investigation employed for superficial and deep swelling in the body. It provides early information regarding the lesions before performing biopsy and aid in the treatment plan of the patient. However sample inadequacy from representative lesion may render patients to repeat aspirations and may also cause delay in reports. There is an ever increasing demand for rapid diagnosis in cytology. The present study was conducted to assess the utility of brilliant cresyl blue as a rapid stain in fine needle aspiration cytology. Objective: To assess the utility of rapid Brilliant cresyl blue (BCB) stained wet mount preparation in routine fine needle aspiration cytology. Materials and Methods : The study was carried out in the Cytology laboratory, Govt. Medical College Thiruvananthapuram and PG Pathology Lab, Department of MLT, Govt. Medical College Thiruvananthapuram for a period of six months. A total of 50 cases referred to cytology laboratory for FNAC were included in this study. A wet mount preparation with Brilliant cresyl was prepared immediately after aspiration and examined for cellular adequacy and cytomorphology, which was then compared with the routine Papanicolau stained smear . Results & Conclusion:In situations for rapid diagnosis, and to assess the cellular adequacy of a lesion Brilliant cresyl blue staining method is very helpful. Most of the cases diagnosed as malignant on Brilliant cresyl blue staining correlate with the Pap stain. All the cases diagnosed as benign on rapid brilliant cresyl blue stain were also diagnosed as benign with Pap stain. The result clearly indicate that the brilliant cresyl blue stained wet mount preparation can be used as a rapid supplementary diagnostic procedure in FNAC at least for urgent reporting and sample adequacy assessment.

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“…In this study, the on-site evaluation was done by cytopathologists after staining the smears with toluidine blue. Although there are a few similar studies available that have used Diff-Quick, ultrafast Papanicolaou, and brilliant cresyl blue stains,[ 2 ] one study by Hemalatha et al . [ 3 ] went one step further and tried to evaluate the adequacy on unstained smears.…”

mentioning

confidence: 99%

Use of cell block preparation for improving adequacy of cytology smears

Chowhan

Rukmangadha

Patnayak

et al. 2015

J Cytol

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On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

Cited by 4 publications

References 6 publications

Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

Utility of rapid brillant cresyl blue stained wet mount preparation in routine fine needle aspiration cytology

Use of cell block preparation for improving adequacy of cytology smears

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