On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine

Rana, Vandana; Eckard, Anahita Dehkhoda; Teller, Philip J.; Ahring, Birgitte Kiær

doi:10.1016/j.biortech.2013.12.059

Cited by 81 publications

(42 citation statements)

References 33 publications

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“…Different agricultural wastes (e.g. sugar cane bagasse, corn stover, elephant grass, fruit pomace and straw or bran from wheat, rice or corn) have been applied for enzyme production [28,[57][58][59][60][61][62][63]. These agricultural residues can also be used as potential biomass sources for biorefineries, and are therefore ideal for a CBP setup.…”

Section: Medium Composition For Enzyme Productionmentioning

confidence: 99%

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

Hansen

Lübeck

Frisvad

et al. 2015

Process Biochemistry

145

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Section: Medium Composition For Enzyme Productionmentioning

confidence: 99%

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

Hansen

Lübeck

Frisvad

et al. 2015

Process Biochemistry

145

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“…On-site production of enzymes by Trichoderma has been investigated for the hydrolysis of a range of substrates, such as: wheat straw [34][35][36], wet-exploded corn stover [37], sugar cane bagasse [38][39][40], steam-treated spruce [41], rice straw [42,43], wet-exploded loblolly pine [37] as well as newspaper sludge and steam-exploded wood al., [44]. Compared with these substrates, the duckweed species Lemna minor and Spirodela polyrhiza should in theory exhibit lower recalcitrance, due to their simpler structure and low lignin content.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

On-Site Enzyme Production by Trichoderma asperellum for the Degradation of Duckweed

Bech¹,

Herbst²

2015

Fungal Genom Biol

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The on-site production of cell wall degrading enzymes is an important strategy for the development of sustainable bio-refinery processes. This study concerns the optimization of production of plant cell wall-degrading enzymes produced by Trichoderma asperellum. A comparative secretome analysis was performed on T. asperellum growing on PDA agar, wheat bran and duckweed, respectively. T. asperellum proved to be able to produce a wide enzyme profile, including both depolymerization and debranching enzymes, mainly consisting of hemi-cellulases. The secretome analysis showed specific glycoside hydrolase-induction on duckweed compared with growth on wheat bran and PDA, including a GH62 α-L-arabinofuranosidase, a promising candidate enzyme for the degradation of duckweed. The enzyme cocktail from T. asperellum proved capable of degrading pretreated duckweed, obtaining up to 60% of the theoretical glucose yield, making it a potential candidate for on-site enzyme production.

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“…The second reason is that use of lignocellulosic biomass as substrate to induce cellulase production has an increased enzymatic hydrolysis specicity for the substrate itself than others. 14,17,18 This is so-called on-site enzyme production for lignocellulose-based bioreneries. …”

mentioning

confidence: 99%

Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

et al. 2017

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Mixed culture of Trichoderma reesei and Aspergillus niger was employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover. We comprehensively compared the five pretreatments including sodium hydroxide, steam explosion, aqueous ammonia, lime and diluted sulfuric acid in the enzymatic hydrolysis and then in the whole processes from corn stover to single cell oil (SCO). The results were not completely but roughly the same. However, it is conclusive that sodium hydroxide pretreatment was the best one. The process with sodium hydroxide pretreatment could produce 23.5 g dry cell biomass harboring 13.7 g single cell oil (SCO) from 146.2 g corn stover. The total yields of cell biomass and SCO were 0.161 and 0.094 g g À1 corn stover, respectively. This process was proven the most efficient. Through this work, we established efficient SCO production process from corn stover.

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On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine

Cited by 81 publications

References 33 publications

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

On-Site Enzyme Production by Trichoderma asperellum for the Degradation of Duckweed

Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

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