On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies

Rastmanesh, Afagh; Boruah, Jayanta S.; Lee, Min-Seok; Park, Seungkyung

doi:10.3390/bios14030122

Cited by 3 publications

(1 citation statement)

References 141 publications

(186 reference statements)

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“…Colonies were counted in plates containing 10 to 200 colonies for bacteria and 5 to 150 colonies for fungi [50]. The number of colonies was corrected according to the conversion table of the Most Probable Number of Colonies Forming Units (Pr) [52] due to the superposition of microbial particles impacting the same spot through the same sieve pore [48,53]. The bacteria and fungi concentrations were calculated using the methodology described in EN 13098:2019E [50] and according to the following Equation (1).…”

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confidence: 99%

Culturable Bioaerosols Assessment in a Waste-Sorting Plant and UV-C Decontamination

Manuel,

Samardjieva

2024

Sustainability

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Waste-Sorting Plant (WSP) workers are exposed to bioaerosols containing a large variety of bacterial and fungal species, posing a critical health risk that needs to be assessed and mitigated. The present study aimed to evaluate the indoor air quality in a Portuguese WSP and the air decontamination efficiency with UV-C. The concentrations of bacteria and fungi and particulate matter (PM2.5 and PM10), CO2, relative humidity, and temperature were determined at different hours in manual sorting areas (cabin and ramp) in autumn and winter in 2022 and in administrative offices and canteen in the autumn of 2023. The PM2.5 and PM10 concentrations in the air increased with the daily waste-sorting activities, especially inside the cabin, averaging 22 and 42 μg/m3, respectively, while the CO2 concentration was in the range of 343–578 ppm in both sampling sites. The bacterial species were mainly environmental (mesophilic bacteria) rather than human sources. In the waste-sorting areas, the concentration of bacteria was often found to exceed outdoor values by more than 1000 CFU/m3 on average. Additionally, the concentration of fungi indoors was consistently higher than outdoor values, in many cases exceeding 500 CFU/m3. These findings suggest that workers in these areas are frequently exposed to high levels of microbes. The indoor-to-outdoor (I/O) contamination ratios revealed that the air quality inside the administrative offices and the canteen had high pollutant concentrations during some time periods. The worst scenarios were observed in the canteen and offices with high occupancy in the afternoon. UV-C lamps at 253.7 nm and with 5.0 W irradiation power were used in the sorting cabin to test the indoor air and surface decontamination, and the results showed a high bacterial removal efficacy of over 87.6% after one hour of exposure to UV-C. The present study raises the question of whether 37 °C is the optimal incubation temperature for WSP samples since the microorganisms’ habitat before the sampling had a much lower temperature. As the waste-sorting industry expands, these findings show that the air quality of WSPs remains concerning and requires a holistic approach, integrating the working conditions of all personnel and the implementation and monitoring of mitigation measures.

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confidence: 99%

Culturable Bioaerosols Assessment in a Waste-Sorting Plant and UV-C Decontamination

Manuel,

Samardjieva

2024

Sustainability

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Microbial ocean-atmosphere transfer: the influence of sewage discharge into coastal waters on bioaerosols from an urban beach in the subtropical Atlantic

Rabelo,

Carvalho,

Rebouças

et al. 2024

Marine Environmental Research

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Multi-scenario surveillance of respiratory viruses in aerosols with a sub-single molecule spatial resolution

Liu,

Li,

Lin

et al. 2024

Preprint

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Highly sensitive airborne virus monitoring is critical for preventing and containing epidemics. However, the detection of airborne viruses at ultra-low concentrations remains challenging due to the lack of ultra-sensitive methods and easy-to-deployment equipment. Here, we present an integrated microfluidic cartridge that can accurately detect SARS-CoV-2 and various respiratory viruses with a sensitivity of 10 copies/mL. When seamlessly integrated with a high-flow aerosol sampler, our microdevice can achieve a sub-single molecule spatial resolution of 0.83 copies/m3 for airborne virus surveillance. We then designed a series of virus-in-aerosols monitoring systems (RIAMs), including versions of a multi-site sampling RIAMs (M-RIAMs), a stationary real-time RIAMs (S-RIAMs), and a roaming real-time RIAMs (R-RIAMs) for different application scenarios. Using M-RIAMs, we performed a comprehensive evaluation of 210 environmental samples from COVID-19 patient wards, including 30 aerosol samples. The highest positive detection rate of aerosol samples (60%) proved the aerosol-based SARS-CoV-2 monitoring represents an effective method for spatial risk assessment. The detection of 78 aerosol samples in real-world settings via S-RIAMs confirmed its reliability for ultra-sensitive and continuous airborne virus monitoring. Therefore, RIAMs shows the potential as an effective solution for mitigating the risk of airborne virus transmission.

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On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies

Cited by 3 publications

References 141 publications

Culturable Bioaerosols Assessment in a Waste-Sorting Plant and UV-C Decontamination

Culturable Bioaerosols Assessment in a Waste-Sorting Plant and UV-C Decontamination

Microbial ocean-atmosphere transfer: the influence of sewage discharge into coastal waters on bioaerosols from an urban beach in the subtropical Atlantic

Multi-scenario surveillance of respiratory viruses in aerosols with a sub-single molecule spatial resolution

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