On point identification of species origin of food animals by recombinase polymerase amplification-lateral flow (RPA-LF) assay targeting mitochondrial gene sequences

Kumar, Dinesh; Kumar, Rajiv; Rana, Preeti; Mendiratta, S. K.; Agarwal, R. K.; Singh, Praveen; Kumari, Sarita; Jawla, Jyoti

doi:10.1007/s13197-020-04637-6

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…When adulteration is present, the level of sensitivity obtained is sufficient to cope with the actual demand in animal-derived ingredient detection. Some RPA-based methods for detection of animal-derived ingredient had been developed ( Chen et al, 2022 , Ivanov et al, 2021 , Kumar et al, 2021 ). All of them achieved good detection limits or adulteration sensitivity, but none of them studied the effect of background DNA on the detection ability at different adulteration ratio.…”

Section: Resultsmentioning

confidence: 99%

Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Zhou

Wang²,

Xiang³

et al. 2023

Food Chemistry: Molecular Sciences

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Section: Resultsmentioning

confidence: 99%

Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Zhou

Wang²,

Xiang³

et al. 2023

Food Chemistry: Molecular Sciences

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“…As a result of RPA, homogeneous dsDNA product of a given length is obtained. The successful application of RPA in combination with fluorescent and colorimetric detection [56,57] or with ICT [58][59][60][61][62][63][64][65] for detecting the impurities in meat (chicken, duck, pig, etc.) has been described.…”

Section: Isothermal Amplification Methodsmentioning

confidence: 99%

Molecular genetic methods for identifying raw materials in meat products: Diversity, opportunities and prospects

Safenkova,

Vostrikova,

Taranova

et al. 2024

Teor. prakt. pererab. mâsa (Print)

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In the current economic situation, after easing the Covid pandemic restrictions, almost all laboratories, which are focused on evaluation of the conformity of food products, have faced issues in supplying for their laboratories. In this regard, in the last years many laboratories have been forced to validate new approaches and introduce new methods for assessing conformity of the food products. Very often it is not possible to use only one method to resolve the issue of the food product ingredients, especially for the purpose of traceability of their names and the used raw materials, listed on the label. Survey of the raw food materials to determine whether they correspond to the type name is a simpler task, in contrast to survey of the multicomponent food product. Many researchers have to estimate the opportunities and feasibility of application of various methodologies in their workplaces. Therefore, this review is relevant for the researchers in this field, as it focuses on aspects and special features of similar methodologies. The prospect of molecular genetic methods for identification of the raw materials used for manufacturing of meat products is presented below. This review also represents characteristics of methods for identification of the sources of raw materials used for the manufacturing of the meat products, based on the recognition of species-specific sections within the nucleic acids structures. The variety of methods (hybridization methods, polymerase chain reaction, different types of isothermal amplifications, methods using CRISPR/Cas systems), the principles of their implementation, and achieved analytical characteristics are considered. The capacities and competitive potential of various methods are discussed, as well as approaches being developed to overcome the existing limitations.

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“…In addition to real-time data acquisition, the results of the RPA assay can also be visualized by gel electrophoresis or an LF strip. The RPA-LF assay according to Kumar et al (2021) involves hybridization of amplified RPA products with antibody (biotin and fluorescein isothiocyanate) labeled probes. The LF dipstick is added to the product solution, and color formation on the test and control lines is observed (Hsu et al, 2021).…”

Section: Recombinase Polymerase Amplificationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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On point identification of species origin of food animals by recombinase polymerase amplification-lateral flow (RPA-LF) assay targeting mitochondrial gene sequences

Cited by 16 publications

References 22 publications

Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays

Molecular genetic methods for identifying raw materials in meat products: Diversity, opportunities and prospects

A systematic review of DNA‐based methods in authentication of game and less common meat species

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