On „natural” Dna-Rna Complexes in Phage-Infected E. Coli

Konrad, Michael; Stent, Gunther S.

doi:10.1073/pnas.51.4.647

Cited by 19 publications

(2 citation statements)

References 7 publications

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“…Before this can be definitely established, further studies are needed with adenovirus or other animal virus systems. However, other workers employing bacteriophage systems also were unable to demonstrate any complex formation either in vitro (8) or in vivo (18). Therefore, the presence of contaminating denatured DNA may be the more plausible explanation for the conflicting reports.…”

Section: Downloaded Frommentioning

confidence: 97%

Properties of Adenovirus Messenger Ribonucleic Acid Synthesized In Vitro

Warren

1969

J Virol

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Adenovirus deoxyribonucleic acid (DNA) was used as template for the in vitro synthesis of viral-specific ribonucleic acid (RNA). When the kinetics of the reaction were compared by using native and heat-denatured DNA templates, the latter synthesized RNA at a slower rate. The fate of the DNA after acting as template and physical characteritstics of the RNA product were studied. The DNA template, according to its sedimentation rate, was not significantly degraded by the Micrococcus lysodeicticus RNA polymerase. The products of the RNA polymerase reaction had the following properties. (i) Hybridization experiments revealed a high degree of complementarity (50 to 70%) for its homologous DNA. (ii) A very low complementarity (6 to 7%) was found for its heterologous DNA. (iii) The sedimentation rate of the synthetic RNA in a sucrose gradient was 5 to lOS when native DNA was used as the template. When heat-denatured DNA was used, the resulting RNA product, free of the template, sedimented at a rate of 3 to 16S. A rapidly sedimenting (>305) DNA-RNA complex resulted when denatured DNA was the template. The DNA moiety of the complex was sensitive to 125 ,gg of deoxyribonuclease per ml. The RNA of the complex, however, was fully refractory to 50 ,ug of ribonuclease per ml. When the adenovirus DNA was sonically treated and then used as template, the RNA product sedimented at 3 to 9S. The heat-denatured sonically treated DNA template yielded a DNA-RNA complex that also sedimented at an unusually fast rate (>18S).

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Section: Downloaded Frommentioning

confidence: 97%

Properties of Adenovirus Messenger Ribonucleic Acid Synthesized In Vitro

Warren

1969

J Virol

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“…This work has been largely based on the demonstration that these RNA's can form more-or-less stable hybrids with denatured DNA from the same orga nism and presumably are complementary copies of this DNA (205)(206)(207)(208). The hybridization techniques have not yet been fully worked out; certain versions have been seriously questioned (209) , and different versions have not always given the same quantitative results (210, 211). Nevertheless, there does not seem to be any convincing reason for rejecting this work, although it may yet be premature to place absolute reliance on some of its strictly quantita tive aspects.…”

Section: Rna Polymerasementioning

confidence: 98%

Metabolism of Nucleic Acids (Macromolecular DNA and RNA)

Elson¹

1965

Annu. Rev. Biochem.

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Since it is manifestly impossible to cover the entire field of nucleic acid metabolism in one article, I have restricted this review to recent work on enzymes which have to do with macromolecular nucleic acids. Sections are included on the synthetic enzymes DNA polymerase and RNA polymerase and on enzymes which alter the nucleic acids in their macromolecular state, namely, the DNA and RNA methylases. The concluding section deals with degradative enzymes, chiefly RNase, DNase, and polynucleotide phosphoryl ase, but in a much more cursory manner. The review is based on an exten sive, but certainly not complete, coverage of the recent literature. References 1 through 10 are reviews, books, special collections of articles, and critical papers which deal with various aspects of the subject matter of this review. ENZYMES WHICH SYNTHESIZE NUCLEIC ACIDSThe DNA polymerases and RNA polymerases catalyze the synthesis of polynucleotides by the complementary copying of pre-existing polynucleo tides. The precursors are the appropriate nucleoside triphosphates, and a bivalent cation is required (11-16). During the period under review, there have appeared no serious reasons to doubt that these are the principal, and quite possibly the only, enzymes which catalyze the synthesis de novo of DNA and RNA in nature. Beyond this, a great deal remains to be eluci dated, and much of the recent work has dealt with such matters as the chem ical and physical nature of the polynucleotide templates or primers and prod ucts, details of the reaction mechanisms, differences between the reactions in vivo and in vitro, the association of the enzymes with other cell components, and so forth.DNA POLYMERASEThe location of DNA polymerase in the cell.-In bacterial extracts DNA polymerase has been observed to sediment together with DNA (e.g., 17, 18) . In higher organisms, however, a number of studies had indicated that the enzyme is actually located outside the cell nucleus. In view of the unexpected nature of these findings, the matter has been pursued in several laboratories; and reports have now appeared which provide evidence that at least some of

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