On-line SERS detection of adenine in DNA based on the optofluidic in-fiber integrated GO/PDDA/Ag NPs

Gao, Danheng; Yang, Xinghua; Teng, Pingping; Kong, Depeng; Liu, Zhihai; Yang, Jun; Luo, Meng; Li, Zhanao; Wen, Xingyue; Yuan, Libo; Li, Kang; Bowkett, Mark; Copner, Nigel; Wang, Xiaozhang

doi:10.1016/j.snb.2021.129517

Cited by 19 publications

(9 citation statements)

References 35 publications

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“…The intensity of the 733 cm –1 mode decreased with the adenine concentration, and the determined detection limit was 10 –7 M. Table presents a literature survey of the SERS detection limits for adenine utilizing a range of nanoparticles. Depending on the nanoparticles, the SERS detection limit of adenine varies within the range from 10 –6 to 10 –14 M. − In this study, the detection limit appears at the higher adenine concentration range compared with the values reported in the literature. One of the factors influencing the detection limit is the incorporation of the non-SERS-active magnetite component in the MPNPs.…”

Section: Resultsmentioning

confidence: 49%

Multiwavelength SERS of Magneto-Plasmonic Nanoparticles Obtained by Combined Laser Ablation and Solvothermal Methods

Talaikis,

Mikoliunaite,

Gkouzi

et al. 2023

ACS Omega

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The present study introduces a novel method for the synthesis of magneto-plasmonic nanoparticles (MPNPs) with enhanced functionality for surface-enhanced Raman scattering (SERS) applications. By employing pulsed laser ablation in liquid (PLAL) to synthesize plasmonic nanoparticles and wet chemistry to synthesize magnetic nanoparticles, we successfully fabricated chemically pure hybrid Fe 3 O 4 @Au and Fe 3 O 4 @Ag nanoparticles. We demonstrated a straightforward approach of an electrostatic attachment of the plasmonic and magnetic parts using positively charged polyethylenimine. The MPNPs displayed high SERS sensitivity and reproducibility, and the magnetic part allowed for the controlled separation of the nanoparticles from the reaction mixture, their subsequent concentration, and their precise deposition onto a specified surface area. Additionally, we fabricated alloy based MPNPs from Ag x Au 100– x ( x = 50 and 80 wt %) targets with distinct localized surface plasmon resonance (LSPR) wavelengths. The compositions, morphologies, and optical properties of the nanoparticles were characterized by using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), UV–vis spectroscopy, and multiwavelength Raman spectroscopy. A standard SERS marker, 4-mercaptobenzoic acid (4-MBA), validated the enhancement properties of the MPNPs and found an enhancement factor of 2 × 10 8 for the Fe 3 O 4 @Ag nanoparticles at 633 nm excitation. Lastly, we applied MPNP-enhanced Raman spectroscopy for the analysis of the biologically relevant molecule adenine and found a limit of detection of 10 –7 M at 785 nm excitation. The integration of PLAL and wet chemical methods enabled the relatively fast and cost-effective production of MPNPs characterized by high SERS sensitivity and signal reproducibility that are required in various fields, including biomedicine, food safety, materials science, security, and defense.

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Section: Resultsmentioning

confidence: 49%

Multiwavelength SERS of Magneto-Plasmonic Nanoparticles Obtained by Combined Laser Ablation and Solvothermal Methods

Talaikis,

Mikoliunaite,

Gkouzi

et al. 2023

ACS Omega

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“…The Raman EF was calculated using eq normalE normalF = I normalF C normalF I normalN C normalN where I F represents the intensity of the Raman signal in LcARF and I N represents the intensity of the Raman signal on the planar substrate. C F and C N are the concentrations of the 4-MBN molecule.…”

Section: Methodsmentioning

confidence: 99%

“…These weaknesses severely hamper the applications of the Raman-based HcPCF sensor, especially for the real-time monitoring of clinical samples. Other types of optical fibers, such as a metal-lined hollow core fiber and a suspended core optical fiber, have also been investigated for sensing applications; however, the transmission loss is rather high and, sometimes, is not appropriate for specific and label-free detection. − …”

mentioning

confidence: 99%

Giant Enhancement of Raman Scattering by a Hollow-Core Microstructured Optical Fiber Allows Single Exosome Probing

et al. 2023

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Microstructured optical fibers (MOFs) provide solutions for breaking through the bottlenecks in areas of high-power transmission and high-efficiency optical waveguides. Other than transporting light waves, MOFs can synergistically combine microfluidics and optics in a single fiber with an unprecedented light path length not readily achievable by planar optofluidic configurations. Here, we demonstrate that hollow-core anti-resonant optical fibers (HcARFs) can significantly enhance Raman scattering by over three orders of magnitude (EF ≈ 5000) compared with a planar setup, due to the joint mechanisms of strong light−matter interaction in the fiber core and the cumulative effect of the fiber. The giant enhancement enables us to develop the first optical fiber sensor to achieve single cancer exosome detection via a sandwich-structured strategy. This enables a multiplexed analysis of surface proteins of exosome samples, potentially allowing an accurate identification of the cellular origin of exosomes for cancer diagnosis. Our findings could expand the applications of HcARF in many exciting areas beyond the waveguide.

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“…Then, the real-time SERS analysis was conducted on the sample optofluidic chip and the limit of detection was approximately 100 cfu (colony forming unit)/mL. Another recent study reported the use of micro-structured hollow fiber (MHF) for SERS-based adenine sensing and a limit of detection 10 fM was achieved [88].…”

Section: Scattering-based Optofluidic Sensingmentioning

confidence: 99%

Recent Development of Optofluidics for Imaging and Sensing Applications

2022

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Optofluidics represents the interaction of light and fluids on a chip that integrates microfluidics and optics, which provides a promising optical platform for manipulating and analyzing fluid samples. Recent years have witnessed a substantial growth in optofluidic devices, including the integration of optical and fluidic control units, the incorporation of diverse photonic nanostructures, and new applications. All these advancements have enabled the implementation of optofluidics with improved performance. In this review, the recent advances of fabrication techniques and cutting-edge applications of optofluidic devices are presented, with a special focus on the developments of imaging and sensing. Specifically, the optofluidic based imaging techniques and applications are summarized, including the high-throughput cytometry, biochemical analysis, and optofluidic nanoparticle manipulation. The optofluidic sensing section is categorized according to the modulation approaches and the transduction mechanisms, represented by absorption, reflection/refraction, scattering, and plasmonics. Perspectives on future developments and promising avenues in the fields of optofluidics are also provided.

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On-line SERS detection of adenine in DNA based on the optofluidic in-fiber integrated GO/PDDA/Ag NPs

Cited by 19 publications

References 35 publications

Multiwavelength SERS of Magneto-Plasmonic Nanoparticles Obtained by Combined Laser Ablation and Solvothermal Methods

Multiwavelength SERS of Magneto-Plasmonic Nanoparticles Obtained by Combined Laser Ablation and Solvothermal Methods

Giant Enhancement of Raman Scattering by a Hollow-Core Microstructured Optical Fiber Allows Single Exosome Probing

Recent Development of Optofluidics for Imaging and Sensing Applications

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