On-line sample preparation of paraquat in human serum samples using high-performance liquid chromatography with column switching

Lee, Hye Suk; Kim, Kyun; Kim, Jeong Han; Sam, Kyong; Lee, Sang Ki

doi:10.1016/s0378-4347(98)00306-5

Cited by 25 publications

(12 citation statements)

References 21 publications

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“…The major advantage of column-switching system is the direct injection of protein-rich viscous samples, such as plasma, without the need for prior treatment or an internal standard. Several factors such as columns with high efficiency stationary phases, composition of organic solvents and pHs of mobile phase were adjusted to optimize the analysis of azasetron in this study [13,18,19].…”

Section: Resultsmentioning

confidence: 99%

“…As the demand for automation and high-throughput analysis is increased nowadays, on-line sample processing techniques are increasingly used [11]. In particular, a column-switching system allows the direct injection of protein-rich samples, such as plasma, after simple protein precipitation, thereby resulting in a convenient and accurate assay even without an internal standard [12][13][14][15][16]. It has been also reported that column-switching is a powerful technique for the separation and clean-up of multicompartment mixtures, possessing the following main advantages: minimal sample handling, on-line sample processing, considerable time reduction, greater precision and accuracy, improvement in the selectivity by combining different chromatographic modes, avoiding of an internal standard, protection of photo-sensitive analytes and minor consumption of organic solvents [11].…”

Section: Introductionmentioning

confidence: 99%

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Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

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For the quantification of azasetron in rat plasma samples, a column-switching HPLC method was developed and validated. Following dilution of plasma samples with mobile phase A (17 mM potassium phosphate buffer (pH 3.0)) and simple protein precipitation by addition of perchloric acid (60%), the mixture was directly injected onto the pre-column. After endogenous plasma substances were eluted to waste, the analyte was transferred to the trap column by switching the system. Then, the analyte was back-flushed to the analytical column for separation with mobile phase B (a 22:78 v/v mixture of acetonitrile and 17 mM potassium phosphate buffer (pH 3.0)) and detected at 250 nm using a photodiode array detector. A linear standard curve was obtained in the concentration range of 10-800 ng/mL with the correlation coefficient (r) of 0.9998. The intra- and inter-day precision and accuracy values for azasetron were in the ranges of 0.3-12.9% and 89.7-101.4%, respectively. The method was valid in terms of specificity, precision, and accuracy. In addition, this efficient analytical method was successfully applied to determine plasma concentrations of azasetron following oral administration of azasetron at a dose of 4.0 mg/kg to rats.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

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show abstract

“…Several different factors were taken into account to optimize this parameter: columns with high efficiency stationary phases, mobile phases at differents pHs and with different percentages of organic solvent [5,8,16,19]. High organic modifier solvent contents may cause buffer precipitation which can be the cause of clogging in pre-column and tubing.…”

Section: Methods Developmentmentioning

confidence: 99%

Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

Choi¹,

Um²,

Jung³

et al. 2006

Journal of Chromatography B

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“…Column-switching is a technique that directly analyses biological samples at 100/zL levels for microbore LC [16][17][18][19] as well as conventional LC [11,12,20,21]. Column-switching system based on microbore LC for clomipramine can provide higher sensitivity, shorter analysis time and easier sample treatment than other previously reported, column-switching methods for conventional LC [11,12].…”

Section: Semi-microcolumn Switching Systemmentioning

confidence: 99%

“…The feasibility of the system was demonstrated in the analysis of clomipramine in plasma samples (Figure 2). To obtain complete recovery and clean chromatograms in column-switching systems [11,12,[16][17][18][19][20][21], the choice of precolumn packing, washing solvent and valve-switching time is crucial. Capcell Pak MF Ph-1 was an appropriate precolumn for the deproteinization and clomipramine fractionation in column-switching for microbore LC.…”

Section: Semi-microcolumn Switching Systemmentioning

confidence: 99%

Direct analysis of clomipramine in human plasma by microbore high performance liquid chromatography with column-switching

et al. 2000

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SummaryAn automated microbore, liquid chromatographic method with column-switching was developed for the determination of clomipramine from human plasma samples. After direct injection of samples (60#L), plasma proteins and clomipramine were separated in size-exclusion mode using 20 % acetonitrile in 20 mM phosphate buffer (pH 7.0) on Capcell Pak MF Ph-1 precolumn (10 x 4mm I.D.). By valve switching, a fraction containing clomipramine was directed to an intermediate column for subsequent main separation on a microbore Cls column (250 x 1.5 mm I.D.) using 50 % acetonitrile in 20 mM phosphate buffer (pH 2.5) at 0.1 mL min -1. The method was advantageous for rapidity (total analysis time: 15min), reproducibility (C.V. < 4.8 %), and increased sensitivity (1 ng mL-1). The linearity of response was good (r e -> 0.999) over the concentration range 1-250 ng mL -1.

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On-line sample preparation of paraquat in human serum samples using high-performance liquid chromatography with column switching

Cited by 25 publications

References 21 publications

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

Direct analysis of clomipramine in human plasma by microbore high performance liquid chromatography with column-switching

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