On-line reversed–phase liquid chromatography-gas chromatography coupled to mass spectrometry for enantiomeric analysis of chiral compounds in fruit beverages

Caja, M. M.; Blanch, Gracia Patricia; Herráiz, Marta; Castillo, Marı́a Luisa Ruiz del

doi:10.1016/j.chroma.2004.04.050

Cited by 20 publications

(3 citation statements)

References 24 publications

(26 reference statements)

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“…One optical isomer of a drug can provide beneficial pharmacological activity, while the other enantiomer may have a less useful effect or even exhibit other toxic side effects [1][2][3]. Therefore, enantiomeric separation of chiral molecules is becoming important in pharmaceutical industry [4][5][6], clinic chemistry [7,8], environment and food analysis [9][10][11]. Disopyramide, alpha-(2-(diisopropylamino)ethyl)-alphaphenyl-2-pyridineacetamide is a class IA antiarrhythmic drug, which is widely used for an effective prophylaxis and treatment of ventricular and superventricular arrythmias in clinical trials.…”

Section: Introductionmentioning

confidence: 99%

CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide

et al. 2006

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CE with electrochemiluminescence (ECL) detection technique was successfully applied for the chiral separation of a kind of class IA antiarrhythmic racemic drug. To the best of our knowledge, this is the first report of ECL detection used in chiral CE. To get better detection sensitivity and good enantioresolution at the same time, the conditions of capillary inlet and outlet buffer were systematically optimized. Unlike the traditional chiral separation method, the buffers we used in the capillary inlet and outlet differed from each other in terms of buffer pH, ionic strength, type of BGE as well as buffer composition. Under the optimum conditions, baseline enantioseparation and highly sensitive detection of the enantiomers were achieved. Wide linear relationship of each enantiomer was achieved in the range of 5 x 10(-7) to 2 x 10(-5) mol/L with relative coefficients of 0.996 and 0.997, respectively. The detection limits were estimated to be 8 x 10(-8) and 1.0 x 10(-7) mol/L (S/N = 3) for the enantiomers, respectively. In addition, a successful application of this new method to the chiral separation of the racemic drug in spiked plasma samples confirmed the validity and applicability of the chiral CE-ECL method.

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Section: Introductionmentioning

confidence: 99%

CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide

et al. 2006

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“…A programmed temperature vaporizer operated in the solvent split mode has been used to introduce large sample volumes into the GC apparatus (20,35,39), and the efficient transfer of RPLC fractions has been achieved by means of programmed temperature vaporizer-based LC-GC interfaces (8,9,33,34). The through oven transfer adsorption desorption (TOTAD) interface (25,26) has been successfully adapted to on-line RPLC-GC because of its outstanding ability to efficiently eliminate polar .solvents when analyzing complex matrices (11,28,29).…”

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Rapid Recognition of Irradiated Dry-Cured Ham by On-Line Coupling of Reversed-Phase Liquid Chromatography with Gas Chromatography and Mass Spectrometry

Martı́nez

Barba

Calvo

et al. 2011

Journal of Food Protection

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The use of on-line coupling of reversed-phase liquid chromatography and gas chromatography (RPLC-GC) with the through oven transfer adsorption desorption (TOTAD) interface and mass spectrometry (MS) was proposed for testing different types of commercial Spanish dry-cured ham for irradiation treatment at various doses (0, 1.5, 2, and 4 kGy). The qualitative analysis of radiation-specific compounds (e.g., n-pentadecane, 1-hexadecene, 1,7-hexadecadiene, n-heptadecane, 8-heptadecene, and 2-dodecylcyclobutanone) can be simultaneously established in a single run with samples that have or have not been irradiated. The overall analysis, which takes less than 100 min, includes a rapid extraction step using a small amount of dichloromethanemethanol (1:1, vol/vol) and anhydrous sodium sulfate, the subsequent fractionation of the sample in the first dimension of the system (RPLC), the transfer of the target fraction to the second dimension, the GC separation, and the MS detection. The calculated limits of detection in ham were lower than 22 ng/g. Repeatability studies provided relative standard deviation values of 0.8 to 13.5%.

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“…에탄올 발효를 위한 효모 균주는 Saccharomyces cerevisiae KWN [20]을 사용하였으며, YEPD (2% glucose, 1% yeast extract, 2% peptone) 배지에 600 nm에서 측정한 흡광도 (OD 600 ) 기준으로 초기 농도 OD 600 = 1 수준으로 접종하여 30℃에서 24시간 동안 진탕 배양기를 사용하여 전배양하였 다. 초산 발효에 사용한 균주는 Acetobacter pasteurianus KSD5를 [20] [5], 배향(pear flavor)를 나타내는 γ-nonalactone [32], 치 즈향을 나타내는 nonanoic acid, isovaleric acid [33]는 초산 발효 이후 3개월의 숙성 기간 동안 증가하였다. 파인애플 향 을 나타내는 ethyl acetate [34], 바나나 향을 나타내는 isoamyl acetate [35], 꽃 향기를 나타내는 2-phenylethanol 및 ethyl phenylacetate [36]가 전체 대사체 성분 중 차지하 는 비율은 숙성 기간 동안 유의적으로 감소하였다.…”

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Change of Aroma Compounds during Corn Vinegar Ripening

Shin¹,

Park²,

Choi³

et al. 2020

Microbiology and Biotechnology Letters

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Vinegar was prepared from corn produced in Gangwon province and changes to the major aroma components were investigated for acetic acid fermentation and ripening. The most prevalent volatile component in corn alcohol was determined to be 2-phenylethanol (accounting for 27% of total aroma components); the levels of 2-phenylethanol decreased to 15% after acetic acid fermentation. Principal component analysis of volatile compounds revealed that corn vinegar was distinguishable from corn alcohol. The highest content of total volatile components (50%) was acetic acid, followed by 2-phenylethanol and diethyl succinate. Ethyl phenylacetate and diethyl succinate had the highest odor activity value, and might contribute most profoundly to the aroma of corn vinegar.

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On-line reversed–phase liquid chromatography-gas chromatography coupled to mass spectrometry for enantiomeric analysis of chiral compounds in fruit beverages

Cited by 20 publications

References 24 publications

CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide

CE coupling with end-column electrochemiluminescence detection for chiral separation of disopyramide

Rapid Recognition of Irradiated Dry-Cured Ham by On-Line Coupling of Reversed-Phase Liquid Chromatography with Gas Chromatography and Mass Spectrometry

Change of Aroma Compounds during Corn Vinegar Ripening

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