On-line purification and carbon-13 isotopic analysis of carbon dioxide in breath: evaluation of on-line gas chromatography-isotope ratio mass spectrometry

Guilluy, R.; Billion-Rey, F.; Pachiaudi, C.; Normand, Sylvie; Riou, Jean‐Paul; Jumeau, E. J.; Brazier, J.L.

doi:10.1016/0003-2670(92)85368-g

Cited by 48 publications

(37 citation statements)

References 10 publications

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“…For all the enrichments measured by gas chromatographymass spectrometry, the lowest enrichment measurable was 0.1%. 13 C enrichment in the CO 2 of expired gas was measured by isotope ratio mass spectrometry (IRMS) (23).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

Diraison

Yankah

Letexier

et al. 2003

Journal of Lipid Research

112

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We assessed the contributions of human liver and adipose tissue de novo lipogenesis (DNL) to triacylglycerol (TAG) synthesis. Volunteers were fed a high-energy, highcarbohydrate diet (HC, n ‫؍‬ 5) or a normocaloric diet (NC, n ‫؍‬ 10). NC subjects remained in the fasting state (Study 1, n ‫؍‬ 5) or received oral glucose (Study 2, n ‫؍‬ 5) throughout the test (12 h). HC subjects remained in the fasting state

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Section: Analytical Proceduresmentioning

confidence: 99%

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

Diraison

Yankah

Letexier

et al. 2003

Journal of Lipid Research

112

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“…7,[12][13][14] These methods required several steps for purifying headspace gas from water or water and ethanol (whose mass 46 interferes with the CO 2 isotopomers- 12 distillation procedures and cold traps and the use of a dual inlet isotopic ratio mass spectrometer. A continuous flow method has also been described, but it still required a dedicated on-line system with a cryogenic trap and a vacuum line.…”

Section: C4 Plants (Dicarboxylic Acid Route Ormentioning

confidence: 99%

GasBench/isotope ratio mass spectrometry: a carbon isotope approach to detect exogenous CO₂ in sparkling drinks

Cabañero

San-Hipólito

Rupérez

2007

Rapid Comm Mass Spectrometry

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A new procedure for the determination of carbon dioxide (CO 2 ) 13 C/ 12 C isotope ratios, using direct injection into a GasBench/isotope ratio mass spectrometry (GasBench/IRMS) system, has been developed to improve isotopic methods devoted to the study of the authenticity of sparkling drinks. Thirty-nine commercial sparkling drink samples from various origins were analyzed. Values of d 13 C cava ranged from S20.30% to S23.63%, when C3 sugar addition was performed for a second alcoholic fermentation. Values of d 13 C water ranged from S5.59% to S6.87% in the case of naturally carbonated water or water fortified with gas from the spring, and d 13 C water ranged from S29.36% to S42.09% when industrial CO 2 was added. It has been demonstrated that the addition of C4 sugar to semi-sparkling wine (aguja) and industrial CO 2 addition to sparkling wine (cava) or water can be detected. The new procedure has advantages over existing methods in terms of analysis time and sample treatment. In addition, it is the first isotopic method developed that allows Carbon dioxide (CO 2 ) is used to give drinks a slightly acidic and more pleasant taste. 1 CO 2 gas bubbles, either naturally present or added, characterize several beverage products: water, wine, beer, soft drinks, etc. Nowadays, some aspects of CO 2 in beverages, such as purity and concentration, are regulated by European Union (EU) legislation. 2 The CO 2 responsible for effervescence in sparkling wines, which includes French Champagne, Italian Spumante and Spanish Cava, is produced in closed bottles during the second alcoholic fermentation or 'prise de mousse'. CO 2 is produced together with ethanol when yeast ferment sugars.In the last 20 years, stable isotope ratio measurements have been used to prevent fraud in the food products industry. 3 These methods are based on the measurements of the stable isotope ratios of the biologically important elements, 2 H/ 1 H, 13 C/ 12 C, 15 N/ 14 N, and 18 O/ 16 O, by isotope ratio mass spectrometry (IRMS).Carbon stable isotope methods are based on the fact that a molecule (e.g. sugars) exhibits varying 13 C isotope concentrations depending on the plant which has metabolized it and therefore its stable isotope ratio can provide information on its botanical origin. In C3 plants (Calvin cycle) 3-ribulose diphosphate is used to fix the CO 2 , and the 13 C/ 12 C abundance ratios, expressed as the d 13 C values, lie in the À23 to À28% range. 4 C4 plants (dicarboxylic acid route or Hatch-Slack pathway) use oxaloacetate as the CO 2 acceptor and have d 13 C values between À9 and À15%. 5 For several fermented products, CO 2 reflects the botanical origin of the sugars from which the gas originates. 6 Cane sugar is a C4 plant while the grapevine is a C3 plant. It is thus possible to distinguish by IRMS between the CO 2 produced from the fermentation of the sugar present in the grape must and that originating from added cane sugar. 7 Industrial CO 2 used in the food industry has a large range of d 13 C values (1 to À75%), depending on the pr...

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“…12 The results were expressed either in δ 13 C (‰) or in atom% 13 C as previously described. 10,11 The precision of isotopic measurement was ± 0.01 δ 13 C (‰).…”

Section: Isotopic Analysismentioning

confidence: 99%

“…10,11 In brief, gas chromatography was performed on an HP 5710 gas chromatograph (Hewlett-Packard, Avondale, PA, USA). The gas chromatograph was equipped with a Hayesep QS Porapak 50-80 mesh column (Chrompack, Les Ulis, France).…”

Section: Isotopic Analysismentioning

confidence: 99%

Decarboxylation of [1-13C]Leucine by Peroxyl Radicals

Tinardon

Lamrini

Lacan

et al. 1997

Rapid Commun. Mass Spectrom.

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The decarboxylation of [1-13 C]leucine by peroxyl radicals (ROO • ) has been studied by using combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) to follow the production of 13 CO 2 . Peroxyl radicals were obtained by thermal decomposition of a water soluble azo initiator, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The decarboxylation rates measured by GC/IRMS were dependent on [1-13 C]leucine and AAPH concentrations. The leucine partial order was found to be constant over a 60 min. period (0.6) but the AAPH partial order was found to be variable during the same period, from 1.0 (t = 0) to 0.50 (t = 60 min.). The identification of the products of reaction between leucine and peroxyl radicals requires the use of combined gas chromatography/mass spectrometry using the selected-ion monitoring mode. Methyl-3 butanoate was identified from comparison of its mass spectrum with that of a standard.

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On-line purification and carbon-13 isotopic analysis of carbon dioxide in breath: evaluation of on-line gas chromatography-isotope ratio mass spectrometry

Cited by 48 publications

References 10 publications

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

GasBench/isotope ratio mass spectrometry: a carbon isotope approach to detect exogenous CO₂ in sparkling drinks

Decarboxylation of [1-13C]Leucine by Peroxyl Radicals

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On-line purification and carbon-13 isotopic analysis of carbon dioxide in breath: evaluation of on-line gas chromatography-isotope ratio mass spectrometry

Cited by 48 publications

References 10 publications

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

GasBench/isotope ratio mass spectrometry: a carbon isotope approach to detect exogenous CO2 in sparkling drinks

Decarboxylation of [1-13C]Leucine by Peroxyl Radicals

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Product

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GasBench/isotope ratio mass spectrometry: a carbon isotope approach to detect exogenous CO₂ in sparkling drinks