On-line preconcentration and determination of cobalt by DPTH-gel chelating microcolumn and flow injection inductively coupled plasma atomic emission spectrometry

Abstract

This paper reports the development of a new methodology for the determination of cobalt in biological samples by using a flow injection system with loaded DPTH-gel as solid phase to preconcentrate analytes. The procedure is based on the on-line preconcentration of cobalt on a microcolumn of 1,5-bis(di-2-pyridyl)methylene thiocarbohydrazide immobilized on silica gel (DPTH-gel). The trapped cobalt is then eluted with 1% tartaric acid and 1% citric acid (7.1 mL) and determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The analytical figures of merit for the determination of cobalt are as follows: detection limit (3S), 8.5 ng mL(-1); precision (RSD), 5.8% for 100 ng mL(-1) of cobalt; enrichment factor, 13 (using 7.3 mL of sample); sampling frequency, 40 h(-1) using a 60-s preconcentration time. For a 120-s preconcentration time (14.6 mL of sample volume) a detection limit of 5.7 ng mL(-1), an RSD under 5% at 50 ng mL(-1), an enrichment factor of 25, and a sampling frequency of 24 h(-1) were reported. The precision and accuracy of the method were checked by analysis of biological certified reference materials.