On-line monitoring of galactoside conjugates and glycerol by flow injection analysis

Vega, Félix Amárita; Núñez, Carlos Germán; Weigel, B.; Hitzmann, Bernd; Ricci, Juan C. Díaz

doi:10.1016/s0003-2670(98)00389-4

Cited by 23 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5,[7][8][9] GalOx is currently used in several biotechnological applications, due to its selectivity and unique mode of action of producing a reactive aldehyde functionality and hydrogen peroxide. It has been employed, for example, as a possible dental anti-plaque system 10,11 and in various analytical methods for the determination of lactose and other galactosides, [12][13][14] in glycoprotein detection and bioconjugation, 15,16 and in disease diagnostics. 17,18 Likewise, GalOx has been used for chemo-enzymatic polysaccharide functionalization in the production of novel biopolymers and cellulosic materials.…”

Section: Introductionmentioning

confidence: 99%

Effects of temperature and glycerol and methanol‐feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Anasontzis

Pena

Spadiut

et al. 2014

Biotechnology Progress

View full text Add to dashboard Cite

Optimization of protein production from methanol-induced Pichia pastoris cultures is necessary to ensure high productivity rates and high yields of recombinant proteins. We investigated the effects of temperature and different linear or exponential methanol-feeding rates on the production of recombinant Fusarium graminearum galactose oxidase (EC 1.1.3.9) in a P. pastoris Mut+ strain, under regulation of the AOX1 promoter. We found that low exponential methanol feeding led to 1.5-fold higher volumetric productivity compared to high exponential feeding rates. The duration of glycerol feeding did not affect the subsequent product yield, but longer glycerol feeding led to higher initial biomass concentration, which would reduce the oxygen demand and generate less heat during induction. A linear and a low exponential feeding profile led to productivities in the same range, but the latter was characterized by intense fluctuations in the titers of galactose oxidase and total protein. An exponential feeding profile that has been adapted to the apparent biomass concentration results in more stable cultures, but the concentration of recombinant protein is in the same range as when constant methanol feeding is employed. © 2014 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 30:728–735, 2014

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of temperature and glycerol and methanol‐feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Anasontzis

Pena

Spadiut

et al. 2014

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…The enzyme of GalOD easily lost its activity. However, the galactose sensor exhibited good stability under protection of Cu 2+ during the storage period [29]. After using the galactose sensor, it was washed with deionized water, immersed in a solution of 0.4 mM CuSO 4 and kept at 4 • C overnight.…”

Section: 45mentioning

confidence: 99%

Chitosan/Prussian blue-based biosensors

Wang

Zhu

et al. 2003

Meas. Sci. Technol.

View full text Add to dashboard Cite

Chitosan/Prussian blue (PB) based biosensors, including a glucose sensor, a glutamate sensor and a galactose sensor have been developed. The biosensors exhibit excellent performance; in particular, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electro-catalysis of the PB layer to H 2 O 2 . The biosensors have been applied to detect glucose, galactose and glutamate in human blood serum and fermented solution.

show abstract

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel

Akin

Geyik

et al. 2011

Biotechnology Progress

View full text Add to dashboard Cite

This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R(2) = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R(2) = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements.

show abstract

On-line monitoring of galactoside conjugates and glycerol by flow injection analysis

Cited by 23 publications

References 23 publications

Effects of temperature and glycerol and methanol‐feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Effects of temperature and glycerol and methanol‐feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Chitosan/Prussian blue-based biosensors

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Contact Info

Product

Resources

About