On-line monitoring of fungal cell concentration by dielectric spectroscopy

Krairak, Somchai; Yamamura, Keiji; Nakajima, Mikio; Shimizu, Hiroshi; Shioya, Suteaki

doi:10.1016/s0168-1656(99)00008-5

Cited by 17 publications

(7 citation statements)

References 8 publications

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“…The largest utilization of oxygen during the growth of aerobic microorganisms is for respiration. Oxygen acts as a terminal electron acceptor for oxidative reactions to provide energy for cellular activities [112]. Productivity of several fermentations is limited by oxygen availability and it is therefore important to consider the factors, which affect the efficiency in supplying microbial cells with oxygen [113].…”

Section: Effect Of Agitation and Aerationmentioning

confidence: 99%

Monascus spp.: A source of Natural Microbial Color through Fungal Biofermentation

Manan¹,

Mohamad²,

Ariff³

2017

JMEN

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The search for naturally produced substitutes for chemical food colorants has led to a resurgence of interest in pigments synthesized by fungi such as Monascus spp. This fungus has been used in Asia for many centuries as a natural color and flavor ingredient in food and beverages. The red pigments are of particular interest, because red is the most popular food color and true natural pigments suitable for applications in food industries are difficult to obtain. As a result of recent efforts to replace synthetic food dyes with natural colorants, pigments produced by Monascus spp. have attracted worldwide attention. Monascus color, categorized as a natural color, has also been widely used as a food supplement and in traditional medicine. The major objective of this review deals with production of natural microbial color by Monascus spp. and addresses the parameters involved in fungal biofermentation. The fungal strains of Monascus spp. can be either fermented in solid state fermentation (SSF) or in submerged fermentation (SmF). SSF and SmF are two commonly used techniques during various fermentation processes. One important aspect in the development of a biofermentation process is the ability and suitability of the Monascus strain to be employed with a suitable medium.

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Section: Effect Of Agitation and Aerationmentioning

confidence: 99%

Monascus spp.: A source of Natural Microbial Color through Fungal Biofermentation

Manan¹,

Mohamad²,

Ariff³

2017

JMEN

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“…Dielectric spectroscopy was first proposed for on-line biomass monitoring by Harris et al (1987), and has since found acceptance in many laboratory and industrial facilities worldwide. It has been used to monitor animal cell culture (Davey et al, 1988;Degouys et al, 1993;Ducommun et al, 2001a, Guan et al, 1998Noll and Biselli, 1998;Siano, 1997;Zeiser et al, 1999), plant cell culture (Markx et al, 1991) and microbial fermentation Gheorghiu and Asami, 1998;Krairak et al, 1999;Markx and Kell, 1995;Nicholson et al, 1996;November and van Impe, 2000;Penaloza et al, 1992;Siano, 1997).…”

Section: Introductionmentioning

confidence: 99%

On‐line biomass monitoring of CHO perfusion culture with scanning dielectric spectroscopy

Cannizzaro

Gügerli

Marison

et al. 2003

Biotech & Bioengineering

105

102

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In this work, dielectric spectroscopy was used to monitor two CHO perfusion culture experiments (B14 and B16). The capacitance of the cell suspension was recorded every 20 minutes over an excitation frequency range of 0.2 MHz to 10.0 MHz. A phase plot of the capacitance at a low excitation frequency vs. the value at a higher frequency proved to be an accurate indicator of the major transition points of the culture, i.e., maximum cell viability, end of lactate consumption, point of zero viability. For both experiments, the capacitance signal correlated very well (R(2) >0.98) with viable cell number up to concentrations of 1 x 10(7) cells/mL. Visual observation of the capacitance spectra indicated that changes in the capacitance relative to frequency were related to the cellular morphology. A multivariate model was developed using off-line data that could predict the median cell diameter within a single experiment (B14) with an error of 0.34 microm (2%). Upon extension to a subsequent experiment (B16), the predicted error was 1.18 microm (9%).

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“…The overriding goal is to lower quality control costs without lowering effectiveness. Utilization of PAT for real-time batch release reduces the cost Hill and Thommel (1982); Thompson et al (1985); Kole et al (1985) Deutsche Hefewerke Akhnoukh et al (1996); Armiger et al (1984); Li and Humphrey (1991); Scheper et al (1987); Schügerl (2001) Radio frequency impedance Carvell (2003) Dielectric constant (permittivity) Krairak et al (1999) Optical density (turbidity) Junker et al (1994);van Putten et al (1996); Wipf et al (1994); Yamane et al (1992); Zhou and Hu (1994) Hoffmann-LaRoche, Merck…”

Section: Real-time Batch Releasementioning

confidence: 99%

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

Junker

Wang

2006

Biotech & Bioengineering

113

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This review article has been written for the journal, Biotechnology and Bioengineering, to commemorate the 70th birthday of Daniel I.C. Wang, who served as doctoral thesis advisor to each of the co-authors, but a decade apart. Key roots of the current PAT initiative in bioprocess monitoring and control are described, focusing on the impact of Danny Wang's research as a professor at MIT. The history of computer control and monitoring in biochemical processing has been used to identify the areas that have already benefited and those that are most likely to benefit in the future from PAT applications. Past applications have included the use of indirect estimation methods for cell density, expansion of on-line/at-line and on-line/in situ measurement techniques, and development of models and expert systems for control and optimization. Future applications are likely to encompass additional novel measurement technologies, measurements for multi-scale and disposable bioreactors, real time batch release, and more efficient data utilization to achieve process validation and continuous improvement goals. Dan Wang's substantial contributions in this arena have been one key factor in steering the PAT initiative towards realistic and attainable industrial applications.

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On-line monitoring of fungal cell concentration by dielectric spectroscopy

Cited by 17 publications

References 8 publications

Monascus spp.: A source of Natural Microbial Color through Fungal Biofermentation

Monascus spp.: A source of Natural Microbial Color through Fungal Biofermentation

On‐line biomass monitoring of CHO perfusion culture with scanning dielectric spectroscopy

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

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