“…Some sensors, including biomass probe, dissolved oxygen probe, extracellular oxidoreduction potential probe, and gas monitors, are used in fermentation to monitor cell growth, assess aerobic metabolism, estimate NAD + /NADH + ratio, and measure gases (e.g., CO 2 , CH 4 and H 2 ), respectively [4].In addition, biochemical analysers connected to auto-samplers are used for online monitoring of multiple metabolites. Different from discrete sensors, the MS based chemical multisensory systems, named as electronic noses and electronic tongues [5], have been used in both qualitative recognition of multi-component media and quantitative analysis of component concentrations in wine production. With dynamic metabolite data collected using in situ probes and auto-samplers, mathematic modelling can be applied to achieve precise nutrients feed and harvest time in fedbatch fermentation.…”