On-line measurement of oxygen uptake in cell culture using the dynamic method

Abbreviations: DO: dissolved oxygen OTR: oxygen transfer rate OUR: oxygen uptake rateIn the present work volumetric mass transfer coefficient (k L a) was investigated during batch cultivations of Pseudomonas aeruginosa on a nutrient media. The effects of process variables (viz. impeller speed, oxygen flow and geometry of impeller) on the volumetric mass transfer coefficient of oxygen, k L a, in a biocalorimeter (Bio-RC1) was investigated and reported in this research work. The experimental data have been analyzed employing MATLAB to obtain the influences of the process parameters on k L a. An attempt was made to correlate volumetric mass transfer coefficient with metabolic heat production rate at optimized process conditions. The correlation reported in this work would be useful to control and scale up of bioprocesses.

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“…Dynamic method (Singh, 1996) was used for measuring OUR (oxygen uptake rate) and k L a in the calorimeter by using polarographic DO probe.…”

Section: Experimental Determination Of Our Otr and K L Amentioning

confidence: 99%

Oxygen mass transfer studies on batch cultivation of P. aeruginosa in a biocalorimeter

Sivaprakasam¹,

Surianarayanan²,

Swaminathan³

2008

Electron. J. Biotechnol.

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“…A minimum DO value of 2 ppm was maintained through the reaction to ensure aerobic conditions. Oxygen uptake rate (OUR) was calculated using the dynamic method 23…”

Section: Methodsmentioning

confidence: 99%

Bioenergetics and pathway of acid blue 113 degradation byStaphylococcus lentus

Sekar

Surianarayanan

Shanmugam

et al. 2012

Biotechnology Progress

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Bioreaction calorimetric studies of degradation of the dye acid blue 113 by Staphylococcus lentus are reported for the first time. The heat released during the dye degradation process can be successfully measured using reaction calorimeter. Power time and oxygen uptake rate (OUR) profile followed each other suggesting that heat profiles could monitor the progress of the dye degradation in biocalorimetry. The shifts observed in power-time profile indicated three distinct phases of the bioprocess indicating simultaneous utilization of glucose (primary) and dye (secondary carbon source). Secretion of azoreductase enzyme enhanced the degradation process. Optimization of aeration and agitation rates was observed to be vital to efficient dye degradation. The degradative pathway for acid blue 113 by S. lentus was delineated via high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FT-IR), and gas chromatography coupled with mass spectrometry (GC-MS) analyses. Interestingly the products of degradation were found to have low toxicity, as per cytotoxicity measurements.

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“…Assuming that the oxy gen uptake rate (OUR) data and its derivative are available on-line, Lubenova (1996) suggested an adap tive algorithm for the estimation of specific growth rate. Although these algorithms are simple in structure and their stability is proven, they tend to be highly sensitive to initial guesses and the OUR data from off gas analysis can have a high signal-to-noise ratio, particu larly in animal cell cultures where the oxygen demand can be extremely small (Singh, 1996).…”

Section: Bio-process Model Identificationmentioning

confidence: 99%

Time-varying system identification using modulating functions and spline models with application to bio-processes

Ungarala

2000

Computers & Chemical Engineering

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On-line measurement of oxygen uptake in cell culture using the dynamic method

Cited by 47 publications

References 10 publications

Oxygen mass transfer studies on batch cultivation of P. aeruginosa in a biocalorimeter

Oxygen mass transfer studies on batch cultivation of P. aeruginosa in a biocalorimeter

Bioenergetics and pathway of acid blue 113 degradation byStaphylococcus lentus

Time-varying system identification using modulating functions and spline models with application to bio-processes

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