On‐line identification of further flavone C‐ and O‐glycosides from sugarcane (Saccharum officinarum L., Gramineae) by HPLC‐UV‐MS

Abstract

HPLC-UV-MS (APCI-MS/MS and CID/MS) was utilised for the identification of eight additional flavone glycosides from sugarcane (Saccharum officinarum L., Gramineae) extracts of bagasse, leaves and juice ('garapa'). Relevant information about substitution patterns was obtained through UV detection using post-column addition of shift reagents, while tandem MS provided structural information confirming the proposed structures of the C-glycosides vitexin, orientin, luteolin-8-C-(rhamnosylglucoside), 4',5'-dimethyl-luteolin-8-C-glycoside and the isomeric pair schaftoside-isoschaftoside, besides the O-glycosides tricin-7-O-neohesperidoside and tricin-7-O-glycoside.

On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil

“…Our research groups have been working on the analysis of flavonoids from Brazilian food plants, 13,14 emphasizing 101 Tiberti et al Vol. 18, No.…”

“…Our research groups have been working on the analysis of flavonoids from Brazilian food plants, 13,14 emphasizing the use of on-line LC techniques as a tool for the chemical profiling of plant extracts. The hyphenation (coupling) of high-performance liquid chromatography with UV photodiode array detection (LC/UV) and liquid chromatography with mass spectrometry (LC/MS) provide spectrometric information comparable to those recorded for pure constituents.…”

“…Furthermore, the composition of flavonols in Brazilian apples has still only been partially investigated. Arabbi et al 12 quantified the flavonol content in Gala, Golden, and Fuji samples cultivated in Brazil, using aglycone quercetin as a standard, but did not elucidate the structure of these flavonols.Our research groups have been working on the analysis of flavonoids from Brazilian food plants, 13,14 emphasizing 101 Tiberti et al Vol. 18, No.…”

Validação de método e determinação espectrométrica dos flavonoides das folhas e do vinhoto da cana-de-açúcar e comparação com método CLAE-UV

“…8,9 O melhor tempo de extração foi definido através do estudo da cinética de maceração, testando-se os intervalos de tempo de 1; 3; 6 e 12 h e, posteriormente, os intervalos de tempo de 1; 2; 2,5 e 5 h.…”

“…5 Estudos na literatura têm demonstrado que resíduos agroindustriais como o bagaço e as folhas da cana-de-açúcar (S. officinarum L., Gramineae) são fontes promissoras de flavonoides. [6][7][8][9] No Brasil, durante o processo de colheita manual as folhas da cana são queimadas e, consequentemente, descartadas. Com a queima desta biomassa por longo período, inúmeras partículas e gases poluentes estão sendo enviados à atmosfera, contribuindo para potenciais impactos ambientais como o aquecimento global, a formação de ozônio troposférico, o fenômeno de acidificação (designado por chuva ácida) e a toxicidade humana.…”