On-line (HPLC-NMR) and Off-line Phytochemical Profiling of the Australian Plant, <i>Lasiopetalum macrophyllum</i>

Timmers, Michael; Urban, Sylvia

doi:10.1177/1934578x1100601114

Cited by 7 publications

(4 citation statements)

References 46 publications

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“…The application of HPLC-NMR has been demonstrated as being able to offer valuable insight into the structure class and the range of secondary metabolites present in a crude extract. This has been successfully demonstrated in a number of chemical profiling studies conducted within our research group both on marine and terrestrial extracts and also in monitoring chemical interconversions [1,2,3,4,5]. HPLC-NMR is advantageous in that it has the ability to separate components in situ and is a non-destructive technique.…”

Section: Introductionmentioning

confidence: 98%

Application of HPLC-NMR in the Identification of Plocamenone and Isoplocamenone from the Marine Red Alga Plocamium angustum

2012

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A combination of on-line HPLC-NMR and off-line chemical investigations has resulted in the identification of the previously reported polyhalogenated monoterpene plocamenone, together with the new structural analogue isoplocamenone from the crude extract of the marine alga Plocamium angustum. On-flow and stop-flow HPLC-NMR analyses (including the acquisition of WET 2D NMR spectra) rapidly assisted in the identification of the major component plocamenone and in the partial identification of its unstable double bond isomer isoplocamenone. Conventional off-line isolation and structural characterization techniques were employed to unequivocally confirm both structures, leading to a structural revision for plocamenone, as well as to obtain sufficient quantities for biological testing.

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Section: Introductionmentioning

confidence: 98%

Application of HPLC-NMR in the Identification of Plocamenone and Isoplocamenone from the Marine Red Alga Plocamium angustum

2012

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“…As depicted in Figure 1, two new and eleven known compounds were shown, among which known compounds were identified to be gomphrenol‐3‐ O ‐ β ‐D‐glucopyranoside ( 3 ), [13] kaempferol ( 4 ), [14] kaempferol‐3‐ O ‐ β ‐D‐(6 ′′ ‐feruloylglucopyranoside) ( 5 ), [15] kaempferol‐3‐ O ‐(6 ′′ ‐ O ‐( Z )‐ p ‐coumaroyl)‐ β ‐D‐glucopyranoside ( 6 ), [16] kaempferol‐3‐ O ‐(6 ′′ ‐ O ‐( E )‐ p ‐coumaroyl)‐ β ‐D‐glucopyranoside ( 7 ), [17] isorhamnetin‐3‐ O ‐ β ‐D‐apiofuranosyl‐(1→2)‐ β ‐D‐glucopyranoside ( 8 ), [18] potentilin A ( 9 ), [19] isorhamnetin‐3‐ O ‐ α ‐L‐rhamnopyranosyl‐(1→6)‐ β ‐D‐glucopyranoside ( 10 ), [20] gomphrenol‐3‐ O ‐ β ‐D‐glucopyranosyl‐(1→6)‐ β ‐D‐glucopyranoside ( 11 ), [21] kaempferol‐3‐ O ‐ α ‐L‐rhamnopyranosyl‐(1→6)‐ β ‐D‐glucopyranoside ( 12 ), [22] and kaempferol‐3‐ O ‐ β ‐D‐glucopyranoside ( 13 ), [23] by comparison of their spectroscopic data with literatures.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical and Antibacterial Constituents of Edible Globe Amaranth Flower against Pseudomonas aeruginosa

Wang

Qin

et al. 2022

Chemistry & Biodiversity

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Globe amaranth flower, the edible inflorescence of Gomphrena globose L., was used to treat dysentery and ulcer as well as other infectious diseases caused by microbes in Southwest China, but its function and bioactive components need experimental support. In this study, phytochemical constituents and antibacterial bioactivity of globe amaranth flower against P. aeruginosa were carried out. As a result, two new (1 and 2) and eleven known (3 -11) compounds were isolated, in which compounds 4-7 displayed anti P. aeruginosa bioactivity with the minimum inhibitory concentration (MIC) from 0.008 to 0.256 mg/mL. Furthermore, with aid of the scanning electron microscope (SEM) and a superficial skin infection model in mice, the most potent compound 4 can significantly destroy the structure of bacteria in vitro and restore bacterial infection damage in vivo.

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“…Recent advances have been made concerning the improvement in NMR flow probe technology, introduction of new techniques for solvent suppression, or coupling to an online solid-phase extraction unit for concentrating the eluted peaks prior to spectra recording so as to increase the sensitivity (LC-SPE-NMR). This has contributed to improve the sensitivity, so that an increase in its applications should be expected in coming years, also favored by the introduction of cheaper equipment making the technique available to more laboratories …”

Section: Advances In Anthocyanin Analysismentioning

confidence: 99%

Anthocyanins. Plant Pigments and Beyond

Santos‐Buelga

Mateus

Freitas

2014

J. Agric. Food Chem.

114

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Anthocyanins are plant pigments widespread in nature. They play relevant roles in plant propagation and ecophysiology and plant defense mechanisms and are responsible for the color of fruits and vegetables. A large number of novel anthocyanin structures have been identified, including new families such as pyranoanthocyanins or anthocyanin oligomers; their biosynthesis pathways have been elucidated, and new plants with "a la carte" colors have been created by genetic engineering. Furthermore, evidence about their benefits in human health has accumulated, and processes of anthocyanin absorption and biotransformation in the human organism have started to be ascertained. These advances in anthocyanin research were revised in the Seventh International Workshop on Anthocyanins that took place in Porto (Portugal) on September 9-11, 2013. Some selected papers are collected in this special issue, where aspects such as anthocyanin accumulation in plants, relationship with color expression, stability in plants and food, and bioavailability or biological activity are revised.

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On-line (HPLC-NMR) and Off-line Phytochemical Profiling of the Australian Plant, Lasiopetalum macrophyllum

Cited by 7 publications

References 46 publications

Application of HPLC-NMR in the Identification of Plocamenone and Isoplocamenone from the Marine Red Alga Plocamium angustum

Application of HPLC-NMR in the Identification of Plocamenone and Isoplocamenone from the Marine Red Alga Plocamium angustum

Phytochemical and Antibacterial Constituents of Edible Globe Amaranth Flower against Pseudomonas aeruginosa

Anthocyanins. Plant Pigments and Beyond

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