On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia (Gardenia jasminoides Ellis) fruit extracts

He, Wenhao; Li, Xuan; Xu, Huaide; Gong, Ying; Yuan, Fang; Gao, Yanxiang

doi:10.1016/j.foodchem.2010.04.030

Cited by 83 publications

(47 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The scavenging DPPH·was carried out on the basis of the method descrided by He et al (2010). DPPH was dissolved in ethanol to the concentration of 1.75×10 −4 mol/L.…”

Section: Dpph·radical Scavenging Activitymentioning

confidence: 99%

“…However, there was no universal method that could measure the antioxidant capacities of all products accurately and quantitatively (He et al 2012). Currently, on-line HPLC-ABTS· + assay for analyzing free radical scavenging activity was developed based on the decrease in absorbance at 734 nm after post column reaction of HPLC separated antioxidants with ABTS· + (Koleva et al 2010;He et al 2010;Gong et al 2012a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

Self Cite

View full text Add to dashboard Cite

Asparagus residues were used as materials to obtain antioxidant compounds by solid-liquid extraction in this study. The effects of different extraction parameters including extraction solvents, time, temperature and liquid-solid ratio on the contents of total flavonoids, total phenolics and total antioxidant activity were investigated. Antioxidant activity of the extract from asparagus residues was evaluated by HPLC-ABTS· + and the bioactive components were identified by HPLC-MS/MS. The results showed that the extraction yield was significantly influenced (P<0.05) by solvent composition, extraction time and temperature. The appropriate parameters were preferred as extraction solvent of 50 % ethanol with liquid-solid ratio of 30:1, extraction temperature of 80°C and time of 2 h. Antioxidant activity evaluation of the extract indicated flavonoids and phenolics were dominant bioactive compounds. Five antioxidant compounds were identified as ferulic acid, kaempferol, quercetin, rutin and isorhamnetin.

show abstract

“…The scavenging DPPH·was carried out on the basis of the method descrided by He et al (2010). DPPH was dissolved in ethanol to the concentration of 1.75×10 −4 mol/L.…”

Section: Dpph·radical Scavenging Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Except the difference of type among the fragments, the relative intensities of the fragment ions were quite different, which was related to the peak-heights. Along with relevant references (Zhang et al, 2013;Rodrigues and Bragagnolo, 2013;He et al, 2010), four peaks were identified as four isomers of caffeoylquinic acid [3-CQA (I), 5-CQA (II), 4-CQA (III) and cis-5-CQA (IV)], respectively. In the product, 5-CQA is the dominant compound, of which the content was 78.2% and other three chlorogenic acid isomers were 1.9% (3-CQA), 10.1% (4-CQA) and 2.0% (cis-5-CQA), respectively.…”

Section: Quantitative Determinationmentioning

confidence: 99%

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Zhao¹,

Chen²,

Li³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract. After one run treatment with polyamide, the content of chlorogenic acids was 40.3%. The product was further purified using silicagel column chromatography; the content of total chlorogenic acid was increased 2.29-fold from 40.3 to 92.2%. The LC-MS results showed that total chlorogenic acids were made up of four components: 5-CQA, 3-CQA, 4-CQA and cis-5-CQA. The content of 5-CQA was the most (78.2%) and other three chlorogenic acid derivatives were 1.9, 10.1 and 2.0%, respectively.

show abstract

“…Now it becomes a hot topic due to its numerous good effects, such as on the treatment of irritability in febrile diseases, jaundice, acute conjunctivitis, epitasis, haematuria, pyogenic infections and ulcers of the skin, sprains and painful swellings due to blood stasis [1][2][3][4][5][6][7]. It contains mainly iridoid, such as geniposide, genipingentiobioside, gardenoside and geniposidic acid, etc.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of major ingredients of Gardenia fruit by HPLC-MS/TQMS method

et al. 2017

View full text Add to dashboard Cite

An efficient, accurate HPLC-MS/TQMS method was introduced for the quantitative/qualitative simultaneous analysis of main ingredients, namely geniposide and genipingentiobioside, in the Gardenia fruit. The separation was successfully obtained using a C 8 (100mm×2.1mm, 5μm, 30°C) column by gradient elution with ultrapure water as mobile phase, where flow rate was set to 0.2 ml/min and detection wavelength at 240 nm. The analytical method was validated and the quantification of active compounds, namely genipingentiobioside and gardenoside, was performed. Linearity, precision, repeatability, stability and recovery were also reported. The quantitative analysis revealed that both main ingredients as geniposide and genipingentiobioside have performed a good linear relationship in 0.1-100 mg/ml concentration range (r=1.00000 and r =0.99998). The average content was measured to be 4.842% with RSD 0.96% for geniposide and 1.1976% with RSD 0.47% for genipingentiobioside in the Gardenia fruit. Accordingly, this method would be feasible for the quantity and quality control of crude drugs.

show abstract

On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia (Gardenia jasminoides Ellis) fruit extracts

Cited by 83 publications

References 29 publications

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Simultaneous analysis of major ingredients of Gardenia fruit by HPLC-MS/TQMS method

Contact Info

Product

Resources

About