On-Line Hemodiafiltration Reduces the Proinflammatory CD14+CD16+ Monocyte-Derived Dendritic Cells

Senescent CD14+CD16+ Monocytes Exhibit Proinflammatory and Proatherosclerotic Activity

“…In this study, we showed that CD14 + CD16 + cells, differentiated in vitro in a Th2-dependent environment, displayed characteristics of senescent cells, with an increase in lysosomal enzymes, such as b-galactosidase, and telomeric shortening. These data suggested that, in vivo, the Th2-dependent environment in the elderly and in patients with chronic inflammatory disease may enhance the survival of activated monocytes that become senescent, a finding that we previously demonstrated in patients with chronic inflammation associated with CKD (8,9). We also compared the surface markers in the CD14 + CD16 + cells obtained from elderly subjects and patients with the in vitro sample, and found that both sets of cells were similar.…”

“…We recently demonstrated that CD14 + CD16 + cells exhibit telomeric shortening and other characteristics of senescent cells (8,9). Several lines of evidence support that senescent cells may play an important role in aging-related diseases and that induction of accelerated senescence in cells under pathological conditions may precipitate aging-associated diseases, such as chronic inflammation (10), premature cardiovascular disease (11), and cancer (12).…”

Effect of Different Dialysis Modalities on Microinflammatory Status and Endothelial Damage

“…It is important to emphasize that our PD patients, in contrast to the results observed in CKD-NonD and HD, did not show microinflammation, as assessed by the percentage of CD14 ϩ CD16 ϩ monocytes. In a recent paper, we suggested that the expansion of CD14 ϩ CD16 ϩ in CKD patients may be a consequence of an ongoing inflammatory process induced by the uremia itself (13). It is generally accepted that hemodiafiltration offers a highly effective dialysis modality that widens the spectrum of uremic toxins removed from small to medium-sized molecular solutes (18 -20).…”

“…In fact, we have demonstrated that on-line hemodiafiltration with a high convective transport reduced the percentage of proinflammatory CD14 ϩ CD16 ϩ cells in comparison with high-flux HD (13). Furthermore, we have also observed that higher levels of CD14 ϩ CD16 ϩ in CKD patients were correlated with high plasma levels of endothelial microparticles, an early feature of endothelial damage (14).…”

Influence of Online Hemodiafiltration on Hemoglobin Level, ESA-Dosage and Serum Albumin – A Retrospective, Multicenter Analysis

“…In the presence of ultrapure dialysis fluid which is ultrafiltrated by endotoxin restraint systems (Weber et al, 2000;Canaud et al, 2001;Minetti et al, 1985;Hakim et al, 1984) and biocompatible high flux dialysis membranes the convective diffusive treatment significantly prevents the complement activation (Braun et al, 1995;Savica et al, 2006;Jorres et al, 1999;Hörl et al, 1986) in the first minutes of olHDF session and removes proinflammatory substances (cytokines) too (Bellomo et al, 1991;Filiopoulos et al, 2008;Haas et al, 2007;Libetta et al, 2007;Mariano et al, 2005). The intra and interdialytic inflammations are reduced (Ramirez et al, 2007;Ramirez et al, 2007;Carracedo et al, 2006). The suppressed inflammatory process during olHDF leads to an increasing serum albumin concentration via an increased synthesis rate.…”