On-line detection of low naphthalene concentrations with a bioluminescent sensor

Valdman, E.; Valdman, Belkis; Battaglini, Fernando; Leite, Selma Gomes Ferreira

doi:10.1016/s0032-9592(03)00248-6

Cited by 17 publications

(14 citation statements)

References 18 publications

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“…Pseudomonas fluorescens HK44 is a bacteria that produces bioluminescent light in response to naphthalene (14,18,19,20), salicylic acid or 4-methyl salicylate when physiological active during a bioremediation process (7). This strain is the first genetically modified organism allowed in the USA for bioremediation field application (12).…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools

Silva¹,

Valdman²,

Valdman³

et al. 2007

Braz. J. Microbiol.

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The optimal conditions for salicylic acid biodegradation by Pseudomonas fluorescens HK44 were determined in this study with the intention to create a microbial sensor. Kinetic experiments permitted a definition of 60 and 30min the time needed to achieve the maximum degradation of salicylic acid presented in a medium with and without yeast extract, respectively. The degradation in medium without yeast extract and the quantification by spectrophotometry 230 nm were selected to be used in further tests. The use of preactivated cells or on the exponential growth phase showed better salicylic acid degradation percentages when compared to nonactivated cells or on the stationary growth state. Finally, the best cellular concentration used on the salicylic acid degradation was 0,1 g.L -1. Strain HK44 shows to be capable of degrade salicylic acid presented in simple aqueous systems, making this strain a promising tool for the application on a luminescent microbial sensor.

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Section: Introductionmentioning

confidence: 99%

Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools

Silva¹,

Valdman²,

Valdman³

et al. 2007

Braz. J. Microbiol.

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“…In a previous work, a low detection limit (0.16 mol L −1 ) and a relative fast beginning of the bioluminescence response (around 20 min) were verified for the bioevaluation of naphthalene in the aqueous phase [26]. The use of this microorganism as a biosensor for naphthalene in the gas phase was evaluated with a dynamic gas generator.…”

Section: Introductionmentioning

confidence: 88%

“…Although a number of bacterial biosensors for naphthalene in aqueous solutions have been proposed before [11,13,18,26,27], the detection of naphthalene in the gas phase by luminescent bacteria has been less explored [9,12,25].…”

Section: Introductionmentioning

confidence: 99%

Bioluminescent sensor for naphthalene in air: Cell immobilization and evaluation with a dynamic standard atmosphere generator

Valdman

Gutz

2008

Sensors and Actuators B: Chemical

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“…The Center of Environmental Biotechnology (CEB) has developed several biocaptors up to the prototype to analyze various pollutants found in water and, among them, some petroleum compounds, BTEXs and naphthalene (Table 1). Other groups have also published results concerning the detection of naphthalene; recently, Valdman et al (2004) built a reporter able to detect naphthalene with a response time of 19 min and with a lower limit of naphthalene detection of 20 µg·l -1 , the health advisory limit for naphthalene fixed by United States EPA (US Environmental Protection Agency) being 20 µg·l -1 . Heitzer et al, 1992 IFP in collaboration with the CEB (PhD project) intends to develop a bioreporter to detect fuel ethers in water.…”

Section: Applications To Petroleum Compounds Detectionmentioning

confidence: 99%

Biosensors for the Environment

Malandain

Fayolle

Bedouelle

2005

Oil & Gas Science and Technology - Rev. IFP

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Résumé -Biocapteurs pour l'environnement -La présence de polluants d'origine industrielle ou agricole dans l'environnement, et dans les eaux en particulier, est liée à l'utilisation massive de composés tels que les herbicides et pesticides, les solvants chlorés ainsi que les composés des essences qui peuvent être déversés lors du transport et du stockage des carburants. Les composés chimiques concernés sont généralement toxiques pour l'homme et les animaux. Cette toxicité nécessite dans les cas de pollution de suivre l'évolution de ces composés (en particulier en cas de menace sur des captages d'eau potable). Les outils classiques de mesure (CPG, CPG/SM, etc.) sont très efficaces du point de vue de la sensibilité et de la reproductibilité mais leur utilisation nécessite d'effectuer des prélèvements sur site, de transporter les échantillons puis de les analyser au laboratoire. Les délais induits peuvent être rédhibitoires et les coûts ne sont pas négligeables quand de nombreux échantillons sont à analyser sur des périodes de temps longues. C'est pour ces raisons que de nouveaux appareils de mesure, biosenseurs ou biocapteurs, qui profitent de l'avancée des connaissances dans plusieurs domaines scientifiques et dans celui de la biologie en particulier, ont été conçus. Ils permettent la création d'outils analytiques aussi fiables mais plus sélectifs et peu encombrants, qui fournissent des mesures en temps réel sur le site étudié. Abstract -Biosensors for the Environment

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On-line detection of low naphthalene concentrations with a bioluminescent sensor

Cited by 17 publications

References 18 publications

Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools

Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools

Bioluminescent sensor for naphthalene in air: Cell immobilization and evaluation with a dynamic standard atmosphere generator

Biosensors for the Environment

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