On-line desalting and carbohydrate analysis for immobilized enzyme hydrolysis of waste cellulosic biomass by column-switching high-performance liquid chromatography

HPLC mapping of second generation ethanol production with lignocelluloses wastes and diluted sulfuric hydrolysis

“…It is highly relevant to emphasize that several researchers, such as Chen et al (2009Chen et al ( , 2010 , using Balfourodendron riedelianum. The reminiscent presence of this carbohydrate indicates that fructose was consumed at a lower rate than glucose during the initial hours of the fermentation assay.…”

“…The reminiscent presence of this carbohydrate indicates that fructose was consumed at a lower rate than glucose during the initial hours of the fermentation assay. Wood species Wood species According to Costa et al (2008), as a result of the catabolic activities of S. cerevisiae, lactic acid was produced during fermentations and consequently ethanol yields were reduced. In current research lactic acid production was detected in Ocotea porosa (0.293 g L ).…”

“…It must be underscored that the acceptability criteria for the acknowledgement of an individual component in validation studies employing highpurity reference samples, required retention times for a given analytic within ± 2% average for each respective standard used to construct the calibration curve for that analytic (CHENG et al, 2010).…”

On-line flow injection analysis using gold particle modified carbon electrode amperometric detection for real-time determination of glucose in immobilized enzyme hydrolysate of waste bamboo chopsticks

“…Although HPLC has the advantage of simultaneous determination of several carbohydrates with good accuracy, precision, and sensitivity, the instrument for HPLC is bulky and expensive and the analysis time is usually too lengthy [13] to perform the real-time monitoring. For real-time monitoring of specific carbohydrate, the amperometric enzyme electrode method is a better technique due to advantages such as substrate specificity, easy operation, low cost, portable instrumentation, and faster analysis [4,5].…”

Validação Da Metodologia Por Cromatagrafia Líquida De Alta Eficiência Acoplada Ao Detector De Índice De Refração Para a Análise De Carboidratos Presentes No Bagaço De Cana-De- Açúcar

“…Os métodos cromatográficos são as técnicas analíticas mais potentes para a análise de carboidratos em xaropes de biomassa lignocelulósica (Cheng et al, 2010). Cromatografia em camada delgada (CCD), cromatografia gasosa (CG) e cromatografia líquida de alta eficiência (CLAE) são comumente utilizadas para separação e identificação dos carboidratos, porém eles são frequentemente quantificados por CLAE usando a detecção por índice de refração (IR) uma vez que esse método é capaz de realizar medições rápidas, sensíveis, precisas e exatas (Raessler, 2011).…”