On-line comprehensive two dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part II: Application to the separation of peptides

D’Attoma, Amélie; Heinisch, Sabine

doi:10.1016/j.chroma.2013.07.048

Cited by 64 publications

(38 citation statements)

References 53 publications

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“…D'Attoma and Heinisch applied RPLC × HILIC for the separation of a tryptic digest of three proteins and compared the results with an RPLC × RPLC separation. The authors concluded that the RPLC × HILIC method did not suffer from peak-shape distortion as a result of overloading, but did suffer from injection effects [111]. Holčapek et al applied RPLC × HILIC-MS for the analysis of complex lipidomic samples [112].…”

Section: Hilicmentioning

confidence: 99%

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

198

139

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Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two‐dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high‐molecular‐weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one‐dimensional liquid chromatography, two‐dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two‐dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two‐dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two‐dimensional liquid chromatography separations.

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Section: Hilicmentioning

confidence: 99%

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

198

139

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“…On-line HPLC x HT-UHPLC separations of tryptic digest of three proteins were recently achieved in both RPLC x RPLC and RPLC x HILIC with UHPLC as 2 D [57]. The peak capacities obtained, corrected for surface coverage, were 3300 (γ = 0.39) and 2600 (γ = 1), respectively, both in 200 min.…”

Section: Lc X Uhplc In Life Sciencesmentioning

confidence: 99%

“…Black squares correspond to data recently published in the literature (see[57] for more information). The dotted line represents the Pareto curve for analysis time and peak capacity.…”

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Theoretical and practical interest in UHPLC technology for 2D-LC

Sarrut

Crétier

Heinisch

2014

TrAC Trends in Analytical Chemistry

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“…There has not been an attractive alternative to RP for many years for the separation of hydrophilic peptides. Hydrophilic interaction liquid chromatography (HILIC) emerged as an appealing chromatographic mode for the separation of polar compounds like small size hydrophilic peptides (D'Attoma & Heinisch, 2013). HILIC uses hydrophilic stationary phases like those traditionally employed in normal phase (NP) (e.g., silica, amino, and cyano) (Buszewski & Noga, 2012) and also new specific ones.…”

Section: Introductionmentioning

confidence: 99%

Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones

González-García

Puchalska

Marina

et al. 2015

Journal of Functional Foods

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Peptides with antioxidant and ACE-inhibitory capacities have been obtained from a plum processing by-product. The aim of this work was to fractionate most active plum hydrolysates, to identify peptides in these fractions, and to evaluate their resistance to gastrointestinal enzymes. Highest antioxidant capacity was observed in the whole Alcalase hydrolysate while peptides yielding the highest ability to inhibit ACE were concentrated in the fraction <3 kDa from the Thermolysin hydrolysate. RP-HPLC–ESI-MS/MS enabled the identification of 13 and 7 potential antioxidant and ACE inhibitory peptides, respectively, in these fractions. Nevertheless, RP-HPLC alone did not guarantee the successful separation of all peptides. The combination of RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) coupled to ESI-Q-TOF-MS/MS enabled the identification of all peptides. Five potential antioxidant and three potential antihypertensive peptides could be additionally identified by HILIC. A total of 7 potential antioxidant peptides and 3 potential antihypertensive peptides resisted a simulated gastrointestinal digestion.Novozymes Spain S.A

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On-line comprehensive two dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part II: Application to the separation of peptides

Cited by 64 publications

References 53 publications

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Theoretical and practical interest in UHPLC technology for 2D-LC

Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones

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