On-line clarification for the measurement of serum glucose in hyperlipidemic specimens

Artiss, Joseph D.; Strandbergh, Donald R.; Sharma, Ajit; Zak, Bennie

doi:10.1016/0009-8981(86)90148-8

Cited by 10 publications

(3 citation statements)

References 4 publications

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“…The use of enzymic clarification of the plasma of lipaemic whole blood for haemoglobin determination and serum for glucose was recently reported (7,8). The same principle was used in the present investigation to clarify lipaemic neonatal serums for the bilirubin assays.…”

Section: Resultsmentioning

confidence: 88%

“…Another obstacle encountered in adapting the previously reported technique to neonatal bilirubin determinations was the large sample to reagent ratio, relative to earlier reports (7,8), which meant dealing with a correspondingly larger amount of triglyceride in the reaction mixture. In addition, because of the photoreactiyity of bilirubin and because this was an off-line process, it seemed reasonable to keep the clarification time s short s possible.…”

Section: Introductionmentioning

confidence: 91%

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Enzymic Clearing of Lipaemic Serum Following Total Parenteral Nutrition: Determination of Neonatal Bilirubin as a Model

Sharma¹,

Artiss²,

Strandbergh³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary: Bilirubin determinations in the newborn infant are one of the many analytical tests that can yield misleading results when the specimen is either iatrogenically or naturally lipaemic. Incorporation of a recently reported enzymic clarification System into a commercially available test kit enables one to conveniently and accurately quantify both total and direct bilirubin within the present procedural characteristics of the Bilirubin Stat Analyser Photometer. This Instrument measures the former directly with bichromatic spectrophotometry and the latter with a conventional diazo-type reaction. The proposed modification of existing reagents allows one to apply the assay to samples with äs niuch äs, and possibly more than 16 g/l of triglycerides which has been introduced into the vascular circulation äs intravenous total parenteral nutrition. Enzymatische Klärung von lipaemischem Serum nach totaler parenteraler Ernährung: Bestimmung von Bilirubin bei Neugeborenen als ModellZusammenfassung: Bilirabinbestimmungen bei Neugeborenen gehören zu den vielen analytischen Tests, die irreführende Resultate ergeben können, wenn die Probe entweder iatrogen oder von Natur aus lipaemisch ist. Die Einbeziehung eines kürzlich beschriebenen enzymatischen Klärungsverfahrens in ein käuflich erhältliches Testbesteck ermöglicht die bequeme und richtige quantitative Bestimmung von gesamtem und direktem Bilirubin im Rahmen der Vorgegebenen Verfahrenscharakteristik des Bilirubin Stat Analyser Photometers. Dieses Gerät mißt das gesamte Bilirubin direkt mit bichromatischer Spektrophotometrie und das direkte Bilirubin mit einer konventionellen Diäzoreaktion. Die vorgeschlagene Modifikation der vorhandenen Reagenzien erlaubt die Anwendung des Verfahrens auf Proben mit 16 g/l oder möglicherweise mehr Triglyceriden, die als intravenöse totale parentale Ernährung in den Kreislauf eingebracht wurden.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 91%

Enzymic Clearing of Lipaemic Serum Following Total Parenteral Nutrition: Determination of Neonatal Bilirubin as a Model

Sharma¹,

Artiss²,

Strandbergh³

et al. 1986

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Relatively more recent analytical attention to this problem of measurement in the presence of turbidity has included spectrophotometric approaches such as bichromatic measurement (39)(40)(41), trichromatic corrective actions (42), and reflectance measurements off of an opaque background (43)(44)(45), as well as ultracentrifugation (46)(47), chemical precipitation of lipoproteins (48,49), chromatographic clearing (50), enzymatic clearing (51)(52)(53)(54)(55)(56)(57), and some other miscellaneous procedures (58)(59)(60). Several of the techniques of the more recent technologies involve on-line systems such as enzymatic clearing (52)(53)(54)(55)(56)(57) or detergent treatment, thus making them amenable to automation. The other techniques for clarification except some spectrophotometric ones for bypassing a clarification step are off-line and must be carried out prior to subjecting the sample to manual or automated instrumentation.…”

Section: Introductionmentioning

confidence: 99%