On-farm epidemiology, virulence profiling, and molecular characterization of methicillin-resistant Staphylococcus aureus at goat farms

Javed, Muhammad Umar; Ijaz, Muhammad; Durrani, Aneela Zameer; Ali, Muhammad Muddassir

doi:10.1016/j.micpath.2023.106456

Cited by 4 publications

(2 citation statements)

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“…A somewhat similar order of gene harbouring was also reported by Pajohesh et al [38], but he reported the highest percentage of blaZ gene, followed by others, which is different from our study. The prevalence of MRSA having mecA gene isolated from different livestock species has been documented in the previous studies conducted in goats [28], bovines [14], sheep [40] and equines [43] in different regions of Pakistan, which are in the range of the current study findings of MRSA. The current study's prevalence of VRSA isolated from bovine origin is higher than in previous studies [19,42].…”

Section: Discussionsupporting

confidence: 65%

“…For molecular identification of resistance-encoding genes, a commercially available DNA Kit (Vivantis Technologies SDN, BHD, Malaysia) was used for the extraction of DNA from all the phenotypic sensitive and resistant S. aureus isolates [28]. Utilising nano-drop assay, quantification of the extracted DNA was also performed.…”

Section: Genotypic Assessment Of Antimicrobialresistant Genesmentioning

confidence: 99%

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Molecular insights into expression and silencing of resistance determinants in Staphylococcus aureus

Ijaz,

Sabir,

Javed

et al. 2024

Tropical Med Int Health

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ObjectiveThis study aimed to investigate the status of antimicrobial‐resistant strains of Staphylococcus aureus in Pakistan, their association in terms of co‐occurrence with the biofilm‐forming genes, resistance profiling and associated discrepancies in diagnostic methods.MethodologyA total of 384 milk samples from bovine was collected by using convenient sampling technique and were initially screened for subclinical mastitis, further preceded by isolation and confirmation of S. aureus. The S. aureus isolates were subjected to evaluation of antimicrobial resistance by phenotypic identification using Kirby–Bauer disc diffusion method, while the genotypic estimation was done by polymerase chain reaction to declare isolates as methicillin, beta‐lactam, vancomycin, tetracycline, and aminoglycoside resistant S. aureus (MRSA, BRSA, VRSA, TRSA, and ARSA), respectively.ResultsThe current study revealed an overall prevalence of subclinical mastitis and S. aureus to be 59.11% and 46.69%, respectively. On a phenotypic basis, the prevalence of MRSA, BRSA, VRSA, TRSA, and ARSA was found to be 44.33%, 58.49%, 20.75%, 35.84%, and 30.18%, respectively. The results of PCR analysis showed that 46.80% of the tested isolates were declared as MRSA, 37.09% as BRSA, and 36.36% as VRSA, while the occurrence of TRSA and ARSA was observed in 26.31% and 18.75%, respectively. The current study also reported the existence of biofilm‐producing genes (icaA and icaD) in 49.06% and 40.57% isolates, respectively. Lastly, this study also reported a high incidence of discrepancies for both genotypic and phenotypic identification methods of resistance evaluation, with the highest discrepancy ratio for the accA‐aphD gene, followed by tetK, vanB, blaZ, and mecA genes.ConclusionThe study concluded that different antibiotic resistance strains of S. aureus are prevalent in study districts with high potential to transmit between human populations. The study also determined that there are multiple resistance determinants and mechanisms that are responsible for the silencing and expression of antibiotic resistance genes.

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