Abstract:Superhydrophobic surface-based optofluidics have been introduced to biosensors and unconventional optics with unique advantages, such as low light loss and power consumption. However, most of these platforms were made with planar-like microstructures and nanostructures, which may cause bonding issues and result in significant waveguide loss. Here, we introduce a fully enclosed superhydrophobic-based optofluidics system, enabled by a one-step microstereolithography procedure. Various microstructured cladding de… Show more
“…Another drawback of CNC machining lies in how small the chip features could be made, an uncommon constraint in microfluidic device manufacturing. For future applications where better surface finish and more unit-area throughput (the maximum number of wells in a certain chip area reasonably visible to the naked eye) are needed, high-resolution 3D printing and injection molding can be good alternatives to the aforementioned manufacturing methods for future production of the Rotation-Chip. For example, injection molding could be an efficient and cost-effective alternative for large-scale fabrication.…”
The transport, distribution, and mixing of microfluidics
often
require additional instruments, such as pumps and valves, which are
not feasible when operated in point-of-care (POC) settings. Here,
we present a simple microfluidic pathogen detection system known as
Rotation-Chip that transfers the reagents between wells by manually
rotating two concentric layers without using external instruments.
The Rotation-Chip is fabricated by a simple computer numerical control
(CNC) machining process and is capable of carrying out 60 multiplexed
reactions with a simple 30 or 60° rotation. Leveraging superhydrophobic
coating, a high fluid transport efficiency of 92.78% is achieved without
observable leaking. Integrated with an intracellular fluorescence
assay, an on-chip detection limit of 1.8 × 106 CFU/mL
is achieved for ampicillin-resistant Escherichia coli
(E. coli), which is similar to our off-chip results.
We also develop a computer vision method to automatically distinguish
positive and negative samples on the chip, showing 100% accuracy.
Our Rotation-Chip is simple, low-cost, high-throughput, and can display
test results with a single chip image, making it ideal for various
multiplexing POC applications in resource-limited settings.
“…Another drawback of CNC machining lies in how small the chip features could be made, an uncommon constraint in microfluidic device manufacturing. For future applications where better surface finish and more unit-area throughput (the maximum number of wells in a certain chip area reasonably visible to the naked eye) are needed, high-resolution 3D printing and injection molding can be good alternatives to the aforementioned manufacturing methods for future production of the Rotation-Chip. For example, injection molding could be an efficient and cost-effective alternative for large-scale fabrication.…”
The transport, distribution, and mixing of microfluidics
often
require additional instruments, such as pumps and valves, which are
not feasible when operated in point-of-care (POC) settings. Here,
we present a simple microfluidic pathogen detection system known as
Rotation-Chip that transfers the reagents between wells by manually
rotating two concentric layers without using external instruments.
The Rotation-Chip is fabricated by a simple computer numerical control
(CNC) machining process and is capable of carrying out 60 multiplexed
reactions with a simple 30 or 60° rotation. Leveraging superhydrophobic
coating, a high fluid transport efficiency of 92.78% is achieved without
observable leaking. Integrated with an intracellular fluorescence
assay, an on-chip detection limit of 1.8 × 106 CFU/mL
is achieved for ampicillin-resistant Escherichia coli
(E. coli), which is similar to our off-chip results.
We also develop a computer vision method to automatically distinguish
positive and negative samples on the chip, showing 100% accuracy.
Our Rotation-Chip is simple, low-cost, high-throughput, and can display
test results with a single chip image, making it ideal for various
multiplexing POC applications in resource-limited settings.
“…Another drawback of CNC machining lies in how small the chip features could be made, an uncommon constraint in microfluidic device manufacturing. For future applications where better surface finish and more unit-area throughput (maximum number of wells in a certain chip area reasonably visible to the naked eye) are needed, high-resolution 3D printing 34 and injection molding 33 can be good alternatives to the aforementioned manufacturing methods for future production of the Rotation-Chip. For example, injection molding could be an efficient and cost-effective alternative for large-scale fabrication.…”
The transport, distribution, and mixing of microfluidics often require additional instruments, such as pumps and valves, which are not feasible when operated in point-of-care (POC) settings. Here, we present a simple microfluidic pathogen detection system known as Rotation-Chip that transfers the reagents between wells by manually rotating two concentric layers without using external instruments. The Rotation-Chip is fabricated by a simple computer numerical control (CNC) machining process and is capable of carrying out 60 multiplexed reactions with a simple 30-degree or 60-degree rotation. Leveraging superhydrophobic coating, a high fluid transport efficiency of 92.78% is achieved without observable leaking. Integrated with an intracellular fluorescent assay, an on-chip detection limit of 1.8×106CFU/mL is achieved for ampicillin-resistantEscherichia coli (E. coli), which is similar to our off-chip results. We also develop a computer vision method to automatically distinguish positive and negative samples on the chip, showing 100% accuracy. Our Rotation-Chip is simple, low-cost, high-throughput, and can display test results with a single chip image, ideal for various multiplexing POC applications in resource-limited settings.
“…As the head loss coefficient could determine the presence of the oscillation of the flow and the scale of multiphase flow determines the physics as shown in other studies 26 , a detailed study to determine the parameters in the lab scale and the microtubule scale is required to fully address the model scaling problem. To facilitate in vivo study, microfabrication 27 and high resolution microstereolithography 28 can be used in the future to make the ADMC with a similar size of the actual dentine tubules. Figure 5 Observed liquid meniscus oscillation at the first 0.008 s in our theoretical model.…”
A deformable microfluidic system and a fluidic dynamic model have been successfully coupled to understand the dynamic fluid–structure interaction in transient flow, designed to understand the dentine hypersensitivity caused by hydrodynamic theory. The Polydimethylsiloxane thin sidewalls of the microfluidic chip are deformed with air pressure ranging from 50 to 500 mbar to move the liquid meniscus in the central liquid channel. The experiments show that the meniscus sharply increased in the first 10th of second and the increase is nonlinearly proportional to the applied pressure. A theoretical model is developed based on the unsteady Bernoulli equation and can well predict the ending point of the liquid displacement as well as the dynamics process, regardless of the wall thickness. Moreover, an overshooting and oscillation phenomenon is observed by reducing the head loss coefficient by a few orders which could be the key to explain the dentine hypersensitivity caused by the liquid movement in the dentine tubules.
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