On-demand deterministic release of particles and cells using stretchable microfluidics

Fallahi, Hedieh; Cha, Haotian; Adelnia, Hossein; Dai, Yiqin; Ta, Hang T.; Yadav, Sharda; Zhang, Jun; Nguyen, Nam‐Trung

doi:10.1039/d1nh00679g

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…201,202 Besides, mechanical stretching was used to modify the dimension of microtrappers in real-time and onsite for the on-demand deterministic release of single cells. 217 Recently, mechanical stretching has been employed to regulate an ultra-stretchable viscoelastic microfluidic device for size-tunable sorting of Haematococcus pluvialis. 218 Cell stretching is a tissue engineering technique that stretches cells and tissues to study biological functions in vitro.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Actuation for flexible and stretchable microdevices

Roshan,

Mudugamuwa,

Cha

et al. 2024

Lab Chip

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Section: Biomedical Applicationsmentioning

confidence: 99%

Actuation for flexible and stretchable microdevices

Roshan,

Mudugamuwa,

Cha

et al. 2024

Lab Chip

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“…We [ 99 ] reported on a stretchable “U‐shaped” microfluidics‐based trapping technology that enabled fluorescence microscopy‐based CTC detection after staining. The platform features several U‐shaped microstructures for single‐cell capture.…”

Section: Micro and Nano Biosensors For Liquid Biopsymentioning

confidence: 99%

Toward Personalized Nanomedicine: The Critical Evaluation of Micro and Nanodevices for Cancer Biomarker Analysis in Liquid Biopsy

Clack

Soda

Kasetsirikul

et al. 2023

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from circulating biomarkers (CBs). CBs may be found in several minimally invasive bodily fluids including (but not limited to) blood, saliva, urine, pleural effusions, stool, and cerebrospinal fluid. CBs may include circulating tumor cells (CTCs), circulating tumor RNA/DNA (ctRNA/ctDNA), microRNAs (miRNAs), long non-coding RNAs (lncRNAs), proteins, exosomes, autoantibodies, all of which, are known for their diagnostic and prognostic potential. [1] Currently, clinicians and centralized hospitals practice tissue biopsy for diagnosis and characterization of the heterogeneity of the solid tumor which is crucial for personalized medicine. [1a] Despite the many decades of clinical practice, tissue-based biopsies are subject to sampling bias, suffer from the issue of low repeatability, and cause immense pain in patients as the process is invasive. One of the major concerns of biopsies, often ignored, is the entrance of tumor cells into the systemic circulation during the tissue-sampling process, leading to tumor metastasis. [2] To overcome these issues, the detection of CBs through liquid biopsy has shown promise as a potential replacement due to the rapid and semi or non-invasive sampling process and the repeated analysis of samples during therapeutic interventions aid in decision-making about a particular patient's progress.Due to the advancement of highly specific sequencing and gene amplification technologies, liquid biopsies can access more relevant cancer biomarkers, providing a much-needed alternative to tissue-based biopsy. [1b] These recent advances have led to the development of many miniaturized/ portable platforms. The clinical potential of these micro and nano devices is rapidly growing, but they face many challenges before transitioning to point-of-care (POC) platforms that are sensitive, rapid, and accurate, as discussed herein.Previously, our group [3] provided a critical review of circulating tumor DNA (ctDNA) and liquid biopsy opportunities, challenges, and recent advances in detection technologies. The review examines the potential of ctDNA as a marker in liquid biopsy, as well as the detection methods, challenges, and considerations. We further [1b] provided a comprehensive review of advanced liquid biopsy technologies for CBs detection. Others Liquid biopsy for the analysis of circulating cancer biomarkers (CBs) is a major advancement toward the early detection of cancer. In comparison to tissue biopsy techniques, liquid biopsy is relatively painless, offering multiple sampling opportunities across easily accessible bodily fluids such as blood, urine, and saliva. Liquid biopsy is also relatively inexpensive and simple, avoiding the requirement for specialized laboratory equipment or trained medical staff. Major advances in the field of liquid biopsy are attributed largely to developments in nanotechnology and microfabrication that enables the creation of highly precise chip-based platforms. These devices can overcome detection limitations of an individual biomarker by detecting multiple markers ...

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“…Soft elastomers bring microfluidics with better stretchability and versatility. For example, Fallahi et al developed flexible and stretchable microfluidic devices based on thin polydimethylsiloxane (PDMS) membranes for tunable cell separation , and deterministic cell release . However, due to the limitations of stretchability of the PDMS material, the maximum stretching rate of this microfluidics is about 70%. , In addition, Sun et al proposed a microfluidic chip using a hybrid silicon rubber .…”

Section: Introductionmentioning

confidence: 99%

Size-Tunable Elasto-Inertial Sorting of Haematococcus pluvialis in the Ultrastretchable Microchannel

Jia,

Wu,

et al. 2023

Anal. Chem.

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Haematococcus pluvialis is a good source of astaxanthin, which reduces oxidation in the human body, treats inflammation, and slows the growth of breast and skin cancer cells. Since the size of H. pluvialis is often closely related to astaxanthin yield, size-based microalgal separation has far-reaching significance for high-value algae extraction and algal directed evolution. In this work, we report a novel size-tunable elasto-inertial sorting of H. pluvialis in the Ecoflex ultrastretchable microfluidic devices. Ecoflex microfluidic chips can deform and be flexible, bringing flexibility and stretchability to microchannels as well as new possibilities for large-scale modulation of channel geometry. Here, the effects of velocity, channel elongation, and particle size on the elasto-inertial migration of particles are systematically studied. We found that channel elongation has a strong regulating effect on particle focusing. In addition, we verified the continuous regulation of the sorting threshold of microalgal cells by stretching the channel, providing technical support for the extraction and directed evolution of high-yield microalgae.

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On-demand deterministic release of particles and cells using stretchable microfluidics

Abstract: Microfluidic technologies have been widely used for single-cell studies as they provide facile, cost-effective, and high-throughput evaluations of single cells with great accuracy. Capturing single cells has been investigated extensively...

Cited by 7 publications

References 45 publications

Actuation for flexible and stretchable microdevices

Actuation for flexible and stretchable microdevices

Toward Personalized Nanomedicine: The Critical Evaluation of Micro and Nanodevices for Cancer Biomarker Analysis in Liquid Biopsy

Size-Tunable Elasto-Inertial Sorting of Haematococcus pluvialis in the Ultrastretchable Microchannel

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