In this paper, granular segregation in a two-compartment cell in zero gravity is studied numerically by DEM simulation. In the simulation using a virtual window method we find a non-monotonic flux, a function which governs the segregation. A parameter is used to quantify the segregation. The effect of three parameters: the total number of particles N , the excitation strength , and the position of the window coupling the two compartments, on the segregation and the waiting time are investigated. It is found that the segregation observed in zero gravity exists and does not depend on the excitation strength . The waiting time τ, however, depends strongly on : Higher the , lower the waiting time . The simulation results are important in guiding the SJ-10 satellite microgravity experiments.