On-chip screening method for cell migration genes based on a transfection microarray

Onuki-Nagasaki, Reiko; Nagasaki, Akira; Hakamada, Kazumi; Uyeda, Taro Q.P.; Fujita, Satoshi; Miyake, Masato; Miyake, Jun

doi:10.1039/b803879a

Cited by 26 publications

(27 citation statements)

References 9 publications

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“…Recently, several groups have focused on developing a new approach 14,15 . For example, Simpson et al 14 created a robotic-driven pin to deliver a precise scratch in confl uent cell monolayers.…”

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confidence: 99%

Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis

et al. 2011

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miRNA globally deregulates human carcinoma. A critical open question is how many miRNAs functionally participate in cancer development, particularly in metastasis. We systematically evaluate the capability of all known human miRNAs to regulate certain metastasis-relevant cell behaviours. To perform the high-throughput screen of miRNAs, which regulate cell migration, we developed a novel self-assembled cell microarray. Here we show that over 20 % of miRNAs have migratory regulation activity in diverse cell types, indicating a general involvement of miRNAs in migratory regulation. MiR-23b, which is downregulated in human colon cancer samples, potently mediates the multiple steps of metastasis, including tumour growth, invasion and angiogenesis in vivo . It regulates a cohort of prometastatic targets, including FZD7 or MAP3k1 . These fi ndings provide new insight into the physiological and potential therapeutic importance of miRNAs as a new class of functional modulators.

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confidence: 99%

Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis

et al. 2011

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“…Cell migration plays a crucial role in various biological processes, including embryogenesis, wound healing, immune responses, tissue development, and cancer metastasization (Onuki-Nagasaki et al 2008). Therefore, understanding the mechanisms underlying cell migration is very important for the development of new therapeutic strategies for controlling invasive tumor cells.…”

Section: Quantifying Cell Migrationmentioning

confidence: 99%

On-chip cell migration assay for quantifying the effect of ethanol on MCF-7 human breast cancer cells

Huang

et al. 2011

Microfluid Nanofluid

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Ethanol consumption is associated with the risk of breast cancer progression; however, the mechanism of relationship has not yet been fully explained. Research on breast cancer cell migration after ethanol stimulation may give hope for a better understanding of the disease and oncotherapy. Conventional cell migration assays such as Boyden chamber and wound-healing assays are easy to conduct for this purpose; however, these assays have inherent limitations. In this study, we quantified the effect of ethanol on MCF-7 hunam breast cancer cells using a microfluidics-based wound-healing assay. Wounds were prepared by partially digesting a confluent cell sheet using parallel laminar flows in the presence of protease trypsin. The cells at the leading edge of the wound remained intact. Cell image analysis indicates that all the cells cultured in the microdevice took on a good morphology and monolayer growth status. Cell viability assay demonstrates that cell viability decreased with the increase in ethanol concentration and treatment time. For 0, 22, 43, and 65 mmol/l of ethanol, cell viability after being cultured for 24 h was 100%, 99.6%, 99.4%, and 98.4%, respectively. Studying MCF-7 human breast cancer cell migration when treated with different ethanol concentrations revealed that the cell migration distance is directly proportional with ethanol concentration. After being cultured for 24 h at 37°C and 5% CO 2 , the maximal cell migration distance was 231, 283, and 332 lm for 22, 43, and 65 mmol/l ethanol, respectively; all results were higher than the blank test (i.e., ethanol-free test, 218 lm). These findings will be beneficial in developing microfluidic device applications for future research on breast tumor therapy in a biomimetic microenvironment and for developing new methods for breast cancer therapy.

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“…The ability to generate pre-determined cell patterns on the substrate surface is a key step for studying tissue formation and cellular function in vitro, which are usually regulated by diverse biological factors including cell-cell communication, cell-matrix interactions, and soluble factors (Attucks and Katula 2001;Campbell et al 2006;Heasley 2001;Javazon et al 2001;Quehenberger 2005). Patterning cells on a specific area with defined shapes are also necessary for investigating the function of cell interaction (Campbell et al 2006;Rosenthal et al 2007) and the migration of cell in substrate (Nakanishi et al 2007;Onuki-Nagasaki et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Patterned PDMS based cell array system: a novel method for fast cell array fabrication

Hsieh

Huang

2010

Biomed Microdevices

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Cell-cell interaction is important in numerous biological processes, including cell growth, cell differentiation and migration. The ability to generate pre-determined cell patterns or cell arrays on a study surface is crucial for cell-cell interaction studies. In this paper, we developed a method for fast cell array fabrication using laser sintering and the hydrophobicity of PDMS films. This approach can be easily adopted and is cost-effective. Hydrophobic PDMS films were fabricated into polymeric chips containing hundreds of microwells. The films were then transferred onto tissue culture surfaces to separate cells in the formation of cell arrays (Patterned PDMS based cell array system, PCAS). We used NIH/3T3 fibroblast cells to demonstrate the feasibility of PCAS. The success of fast fabrication of patterned cell arrays was obtained using different initial cell seeding densities. We also used poorly adherent PC-12 cells to demonstrate the cell-cell communication. Results showed that the method is very useful for studying topics such as cell-cell interaction, cell-substrate interaction or cell migration.

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On-chip screening method for cell migration genes based on a transfection microarray

Cited by 26 publications

References 9 publications

Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis

Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis

On-chip cell migration assay for quantifying the effect of ethanol on MCF-7 human breast cancer cells

Patterned PDMS based cell array system: a novel method for fast cell array fabrication

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