On-chip processing of particles and cells via multilaminar flow streams

Tarn, Mark D.; Lopez-Martinez, Maria J.; Pamme, Nicole

doi:10.1007/s00216-013-7363-6

Cited by 50 publications

(42 citation statements)

References 125 publications

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“…13–15 Several passive approaches have been demonstrated based on deterministic lateral displacement, 16 microstructure-guided railing, 17,18 hydrodynamic filtration, 19–21 pinched flow fractionation, 22 or inertial microfluidics. 23 In these devices, cells or beads passively migrate from the original medium to the wash solution in the microchannels.…”

Section: Introductionmentioning

confidence: 99%

Standing surface acoustic wave (SSAW)-based cell washing

Ding

Mao

et al. 2015

Lab Chip

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Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.

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Section: Introductionmentioning

confidence: 99%

Standing surface acoustic wave (SSAW)-based cell washing

Ding

Mao

et al. 2015

Lab Chip

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“…We further developed a new device design that significantly reduces the exposure time of cells in ferrofluids, from hours to seconds, by taking advantage of the laminar flow nature of liquids in microchannels. 22, 23 The design is explained in detail in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids

et al. 2017

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This paper reports a biocompatible and label-free cell separation method using ferrofluids that can separate a variety of low-concentration cancer cells from cell culture lines (~100 cancer cells/mL) from undiluted white blood cells, with a throughput of 1.2 mL/h and an average separation efficiency of 82.2%. The separation is based on size difference from cancer cells and white blood cells, and is conducted in a custom-made biocompatible ferrofluid that retains not only excellent short-term viabilities, but also normal proliferations of 7 commonly used cancer cell lines. A microfluidic device is designed and optimized specifically to shorten the exposure time of live cells in ferrofluids from hours to seconds, by eliminating time-consuming off-chip sample preparation and extraction steps and integrating them on-chip to achieve one-step process. As a proof-of-concept demonstration, a ferrofluid with 0.26% volume fraction was used in this microfluidic device to separate spiked cancer cells from cell lines at a concentration of ~100 cells/mL from white blood cells with a 1.2 mL/h throughput. The separation efficiencies were 80±3%, 81±5%, 82±5%, 82±4%, and 86±6% for A549 lung cancer, H1299 lung cancer, MCF-7 breast cancer, MDA-MB-231 breast cancer, and PC-3 prostate cancer cell lines, respectively. Separated cancer cells purity was between 25.3% and 28.8%. In addition, separated cancer cells from this strategy showed an average short-term viability of 94.4±1.3% and separated cells were cultured and demonstrated normal proliferation to the confluence even after the separation process. Owning to its excellent biocompatibility and label-free operation, and its ability to recover low concentration of cancer cells from white blood cells, this method could lead to a promising tool for rare cell separation.

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“…13–15 A simple microfluidic particle coating process involves continuously “dipping” particles into streams of multiple reagents and buffer solutions to achieve the desired coating and washing steps in a single device. 16–18 …”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic coating of particles and cells

et al. 2016

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On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar streams of chemicals. Herein, we introduce an acoustofluidic approach for microparticle and cell coating by implementing tilted-angle standing surface acoustic waves (taSSAWs) into microchannels with multiple inlets. The primary acoustic radiation force generated by the taSSAW field was exploited in order to migrate the particles across the microchannel through multiple laminar streams which contained the buffer and coating chemicals. We demonstrate effective coating of polystyrene microparticles and HeLa cells without the need for magnetic labelling. We characterized the coated particles and HeLa cells with fluorescence microscopy and scanning electron microscopy. Our acoustofluidicbased particle and cell coating method is label-free, biocompatible, and simple. It can be useful in the on-chip manufacturing of many functional particles and cells.

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On-chip processing of particles and cells via multilaminar flow streams

Cited by 50 publications

References 125 publications

Standing surface acoustic wave (SSAW)-based cell washing

Standing surface acoustic wave (SSAW)-based cell washing

Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids

Acoustofluidic coating of particles and cells

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