On-Chip Micropatterning of Plastic (Cylic Olefin Copolymer, COC) Microfluidic Channels for the Fabrication of Biomolecule Microarrays Using Photografting Methods

Abstract

This paper reports on the surface modification of plastic microfluidic channels to prepare different biomolecule micropatterns using ultraviolet (UV) photografting methods. The linkage chemistry is based upon UV photopolymerization of acryl monomers to generate thin films (0.01-6 microm) chemically linked to the organic backbone of the plastic surface. The commodity thermoplastic, cyclic olefin copolymer (COC) was selected to build microfluidic chips because of its significant UV transparency and easiness for microfabrication by molding techniques. Once the polyacrylic films were grafted on the COC surface using photomasks, micropatterns of proteins, DNA, and biotinlated conjugates were readily obtained by surface chemical reactions in one or two subsequent steps. The thickness of the photografted films can be tuned from several nanometers up to several micrometers, depending on the reaction conditions. The micropatterned films can be prepared inside the microfluidic channel (on-chip) or on open COC surfaces (off-chip) with densities of functional groups about 10(-7) mol/cm2. Characterization of these films was performed by attenuated-total-reflectance IR spectroscopy, fluorescence microscopy, profilometry, atomic force microscopy, and electrokinetic methods.

Highly efficient Suzuki cross-coupling reaction within an open channel plastic microreactor immobilized with palladium complexes

“…As shown in ATR-FTIR spectra (Fig. 3), for unmodified COC, the observed IR bands in the range of 2,945 and 2,868 cm -1 were attributed to C-H stretches and the peak around 1,457 cm -1 was for the C-H bend, which were in good agreement with that reported previously (Pu et al 2007). After being modified with DMAPMA, the bands around 1,641 and 1,539 cm -1 could be attributed to amide groups.…”

“…The COC plates and the microchips were fabricated through easily operational and inexpensive hot-embossing technique reported previously (Tsao and DeVoe 2009;Steigert et al 2007;Pu et al 2007). The inner surface of microchannel was modified by photographting (Mair et al 2006;Bedair and Oleschuk 2006;Rohr et al 2003;Samuel et al 2010).…”

Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

“…Several techniques have been proposed to functionalize ZR with carboxylic or amine functional groups using both standard liquid phase chemistry as well as plasma enhanced chemical vapour deposition (PECVD) [3][4][5][6][7][8][9]. The quality of the functional films produced on ZR surface has a significant influence on the performance of the biosensor devices.…”

Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

“…Zeonor [1,2], a cyclo olefin polymer is one such material that has quickly found a widespread use in optics, medical and electrical devices [3][4][5][6]. Fabrication of micro-channel structures on *Revised Manuscript Click here to view linked References cyclo olefin polymer or similar substrates by MEMS technologies among others is relatively well documented [7][8][9]. The fabrication methods include direct bonding techniques [10,11], for which the substrate must be photochemically activated, typically by plasma or UV/ozone oxidation [12,13].…”