On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation

Sugino, Hirokazu; Ozaki, Kazuto; Shirasaki, Yoshitaka; Arakawa, Takahiro; Shoji, Shuichi; Funatsu, Takashi

doi:10.1039/b815765k

Cited by 39 publications

(32 citation statements)

References 23 publications

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“…Miniaturizing standard laboratory processes and integrating them on a LoC offers many benefits: the quantity of analytes is smaller, operations are not time-consuming and measurement sensitivity is improved (Whitesides 2006). Most research is focused on enhancing procedures such as polymerase chain reaction (PCR) for nucleic acids amplification (Obeid et al 2003), cellular counting (Schafer et al 2009), blood cleansing (Yung et al 2009), cells sorting (Sugino et al 2009), human plasma separation (Tachi et al 2009) and immunoassays (Lee et al 2009). Microfluidic devices can be fabricated from a variety of materials.…”

Section: Introductionmentioning

confidence: 99%

Femtosecond laser fabrication and characterization of microchannels and waveguides in methacrylate-based polymers

et al. 2011

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Femtosecond laser ablation is a promising method for producing polymeric microfluidic devices: it is a high precision processing technology resulting from an efficient energy deposition, while simultaneously minimizing heat conduction and thermal damage to the surrounding material. This work reports on the characterization of microchannels and waveguides fabricated by femtosecond laser technology in methacrylate-based polymers, precisely in thermoplastic poly(methyl methacrylate) (PMMA) and in a new material based on a high efficiency UV-curing process of methacrylic monomers bearing hydrophilic polyethylene glycol chains, namely tetraethylene glycol dimethacrylate and poly(ethylene glycol) methacrylate (PEG-MA). Microchannels in PMMA and PEG-MA, fabricated by parallel multi-scans, have sharp edges and low roughness, as investigated by Environmental Scanning Electron Microscopy and laser profilometer. Surface and physico-chemical properties after fs-laser processing were further studied by contact angle measurements and Attenuated Total Reflectance FTIR spectroscopy. Moreover, preliminary tests showed the refractive index of fs-laser PEG-MA exposed zones is different with respect to that of the surrounding polymer, suggesting that PEG-MA can be a good candidate to manufacture microfluidic devices containing integrated optic elements.

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Section: Introductionmentioning

confidence: 99%

Femtosecond laser fabrication and characterization of microchannels and waveguides in methacrylate-based polymers

et al. 2011

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“…by use of a broadband light source such as a Xe lamp and extracting the multiple wavelengths with monochromators and complex optical schemes to achieve an unambiguous separation of different wavelengths. Other reports include the use of multiple photodiodes [7], external wavelength selective gratings [8], color CCD arrays [9], or external spectrum analyzers [10]. …”

Section: Introductionmentioning

confidence: 99%

Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip

Dongre

Weerd²,

Bellini

et al. 2010

Biomed. Opt. Express

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We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.

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“…DEP has also been used to sort dropletencapsulated cells in a microfluidic fluorescence-activated sorter (131) at a rate of 2,000/s. A laser-activated thermoreversible polymer was used for flow switching to sort bacteria from microspheres (132), although the throughput achieved was on the order of 0.3 cells/s (133). Optical forces have also been used to control cell routing on microfluidic chips, with response times on the order of 2-4 ms (134).…”

Section: Future Challengesmentioning

confidence: 99%

Microfluidic impedance‐based flow cytometry

Berardino²,

et al. 2010

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Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance-based characterization. Such systems are being developed to provide simple, low-cost, label-free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro-organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high-content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance-based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip-based cytometry can make a large impact. ' 2010International Society for Advancement of Cytometry Key terms microfluidics; impedance characterization; label free; single cell analysis; hydrodynamic focusing; sorting MICROFLUIDIC flow cytometers can bring many advantages to the field of flow cytometry (FCM). Compared to typical flow cytometry channel sizes, the miniaturized dimensions permit microfluidic systems to analyze single cells, to identify cellular variability in gene expression, or drug response within a cell population. Chipbased cytometers can have lower size and costs than conventional benchtop instruments, and may be portable. Today, the developmental aim for microfluidic systems is to reach the same sensitivity and capability for multiparametric analyses as delivered by conventional flow cytometers. Many efforts have been made to improve existing devices and to create new miniaturized high-end instruments. Microfluidic chips can incorporate on-chip cell preparation, cell culture, lysis, and modules for optical, electrophoretic, or genomic analysis (1). They are also suitable for analysis of cells in suspension as well as adherent cells.Miniaturized cytometry devices will have high impact in the development of point-of-care devices in developing countries. Accurate CD4 1 T-cell counts are used to monitor human immunodeficiency virus (HIV)-infected patients, and various thresholds of the number of CD4 1 T lymphocytes per ll of whole blood are used to start antiretroviral therapy (2-5). A simple, single-purpose CD4 cell counting device, which does not require standard laboratory equipment or trained laboratory personnel, could help some of the 33 million HIV-infected people worldwide monitor the stage of infection (6)(7)(8). In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniatu...

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On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation

Cited by 39 publications

References 23 publications

Femtosecond laser fabrication and characterization of microchannels and waveguides in methacrylate-based polymers

Femtosecond laser fabrication and characterization of microchannels and waveguides in methacrylate-based polymers

Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip

Microfluidic impedance‐based flow cytometry

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