On-chip lysis of mammalian cells through a handheld corona device

Escobedo, Carlos; Bürgel, Sebastian C.; Kemmerling, Simon; Sauter, Nora; Braun, Thomas; Hierlemann, Andreas

doi:10.1039/c5lc00552c

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…Escobedo et al [ 90 ] showed electrical lysis of cells inside a microfluidic chip using a hand held corona device. They were able to lyse baby hamster kidney cells (BHK), enhanced green fluorescent protein human-CP cells (eGFP HCP) 116 and non-adherent K562 leukemia cells completely inside a microfluidic channel.…”

Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

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A Review on Macroscale and Microscale Cell Lysis Methods

2017

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The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.

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Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

“…Cells were lysed using a hand held corona device by applying electric field at the inlet of the device; ( b ) bright field and fluorescent images of before and after of lysis of K562 cells. Reproduced with permission from [ 90 ].…”

Section: Figurementioning

confidence: 99%

A Review on Macroscale and Microscale Cell Lysis Methods

2017

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“…The theories behind corona discharge in liquids are diverse. Escobedo et al [ 61 ] used a handheld corona device, as shown in Figure 4 B (electric discharge of 10 to 30 kV: 300 ms), to lyse baby hamster kidney cells (BHK), enhanced green fluorescent protein human-CP cells (eGFP HCP) with an embedded microelectrode, and non-adherent K562 leukemia cells without microelectrodes.…”

Section: Lysis Techniquesmentioning

confidence: 99%

“…( B ) Schematic of the experimental setup using corona discharge. Reproduced from [ 61 ] with permission of The Royal Society of Chemistry.…”

Section: Figurementioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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“…Bag et al [11] developed an improved method for the extraction of community DNA from different environmental and human origin samples by a combination of physical, chemical, and mechanical lysis methods for proper lysis of microbial DNA. A novel approach to lyse cells on-chip through the application of electric discharges can be used in plasmid recovery [12]. The combined action of enzymes, detergents, and high temperatures constitutes an interesting alternative for the large-scale disruption of E. coli cells and plasmid release [13].…”

Section: Introductionmentioning

confidence: 99%

Efficient Disruption of Escherichia coli for Plasmid DNA Recovery in a Bead Mill

et al. 2017

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Featured Application: In general, the alkaline lysis is more acceptable than the mechanical lysis for pDNA recovery due to the risk of product degradation when the latter is used. In this work, the experimental design allowed us to explore mechanical disruption conditions under which such an effect was minimized. The application of this technique is important for novel pDNA-vaccine process development, since bead milling is one of the most preferable mechanical cell lysis methods on an industrial scale due to its scalability, ease of operation, controllability, and ability to load concentrated cell slurry. Abstract:The release kinetics of pDNA in a bead mill was studied. Samples taken during the process were analyzed to determine total pDNA (pDNA(t)) and supercoiled pDNA (pDNA(sc)) concentration. In order to identify important variables of the process and to develop an empirical model for optimal pDNA(t) and pDNA(sc) release, a two level 2 3 factorial design was used with variables: mill frequency, cell concentration, and bead size. The results were analyzed by response surface methodology. The optimized conditions for pDNA(t) yield 13.26 mg/g dcw (93.41% recovery), with a mill frequency of 30 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 20 g wcw/L. However, the optimized conditions for pDNA(sc) yield 7.65 mg/g dcw (92.05% recovery), with a mill frequency of 15 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 10 g wcw/L. Cell disruption in a bead mill was proved efficient for the release of pDNA(t) and pDNA(sc) compared to the alkaline treatment. The results obtained suggest a compromise between pDNA(sc) purity and recuperation in the process development.

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On-chip lysis of mammalian cells through a handheld corona device

Cited by 12 publications

References 46 publications

A Review on Macroscale and Microscale Cell Lysis Methods

A Review on Macroscale and Microscale Cell Lysis Methods

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Efficient Disruption of Escherichia coli for Plasmid DNA Recovery in a Bead Mill

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