On-chip immunomagnetic separation of allergens from myofibrillar proteins of seafoods for rapid allergy tests

Wang, Li; Bi, Hongyan

doi:10.1039/d2an00813k

Cited by 3 publications

(5 citation statements)

References 38 publications

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“…The same research group have similarly demonstrated the use of IMS of allergens from model sea foods, Larimichthys crocea , Portunus triuberculatus , and Penaeus monodon followed by tryptic digest, after which they were identified by MALDI with a 0% false positive rate. The authors state the identified allergens may be used as markers for the rapid identification of potential allergens in food as well as application to a single drop of human blood for food allergy tests (Wang & Bi, 2022).…”

Section: Methodsmentioning

confidence: 99%

Food allergen analysis: A review of current gaps and the potential to fill them by matrix‐assisted laser desorption/ionization

Birse,

Burns,

Walker

et al. 2023

Comp Rev Food Sci Food Safe

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Food allergy remains a public health, business, and regulatory challenge. Risk analysis (RA) and risk management (RM) of food allergens are of great importance and analysis for food allergens is necessary for both. The current workhorse techniques for allergen analysis (enzyme linked immunosorbent assay [ELISA] and real‐time polymerase chain reaction) exhibit recognized challenges including variable and antibody specific responses and detection of species DNA rather than allergen protein, respectively. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) enables protein identification, with potential for multiplex analysis and traceability to the System of International units (SI), aiding global measurement standardization. In this review, recent literature has been systematically reviewed to assess progress in LC–MS/MS and define the potential and benefits of matrix‐assisted laser desorption/ionization–time‐of‐flight MS (MALDI–ToF‐MS) technology for allergen analysis. MALDI–ToF‐MS of initially intact protein is already applied to verify in silico‐derived peptide sequences for LC–MS/MS analysis. We describe the origins of MALDI and its future perspectives, including affinity bead‐assisted assays coupled to MALDI. Based on the proliferation of reliable and reproducible MALDI‐based clinical applications, the technique should emulate the detection capability (sensitivity) of established allergen detection techniques, whilst reducing technical support and having equivalent multiplexing potential to competing techniques, for example, LC–MS/MS and ELISA. Although unlikely to offer inherent SI traceability, MALDI‐based allergen analysis will complement existing MS approaches for allergens. Affinity bead‐MALDI appears capable of higher throughput at lower cost per sample than almost any existing technique, enabling repeated sub‐sampling as a way to reduce representative sampling issues.

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Section: Methodsmentioning

confidence: 99%

Food allergen analysis: A review of current gaps and the potential to fill them by matrix‐assisted laser desorption/ionization

Birse,

Burns,

Walker

et al. 2023

Comp Rev Food Sci Food Safe

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show abstract

“…Additionally, parvalbumin beta, cytochrome c, and protein S100 were also detected from large yellow croaker (L. crocea) muscle food under different protein extraction protocols in previous studies. 7,8,11 It can be found that an increased number of proteins can be detected from the exudate sample of fish muscle. This may be because the exudate of fish muscle keeps a native environment for proteins.…”

Section: Microfluidics and Maldi-tof Ms-based Identification Of Aller...mentioning

confidence: 99%

“…6 Our previous findings showed that it is reliable to identify the allergens in seafood samples by virtue of MALDI-TOF MS, which was demonstrated by analyzing allergens from sarcoplasmic protein solutions of sablefish (Anoplopoma fimbria), 7 and myofibrillar proteins of seafoods including large yellow croaker (L. crocea), black tiger shrimp (Penaeus monodon), and swimming crab (Portunus trituberculatus). 8 Microfluidic technology has paved a way for effective and quick detection of food allergenic proteins. Microfluidic platforms, integrated with fluorescence detection, electrochemistry, or other detection methods, have been established for detecting allergens in foods.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Our previous findings showed that it is reliable to identify the allergens in seafood samples by virtue of MALDI-TOF MS, which was demonstrated by analyzing allergens from sarcoplasmic protein solutions of sablefish (Anoplopoma fimbria), and myofibrillar proteins of seafoods including large yellow croaker (L. crocea), black tiger shrimp (Penaeus monodon), and swimming crab (Portunus trituberculatus) …”

Section: Introductionmentioning

confidence: 99%

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On-Chip Discovery of Allergens from the Exudate of Large Yellow Croaker (Larimichthys Crocea) Muscle Food by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Lin,

Soko,

Xie

et al. 2023

J. Agric. Food Chem.

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“…In recent years, some of the developed biosensing approaches for food-borne allergen detection have been designed to analyze multiple allergens, ELISA and lateral flow immunoassay (LFIA) being the most relevant ones [ 7 , 8 , 9 , 10 , 11 ]. The former has a high sensitivity to various peanut allergens [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Microimmunoassay (LFµIA) for the Reliable Quantification of Allergen Traces in Food Consumables

et al. 2022

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Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative to mass spectrometry and enzyme-linked immunosorbent assay (ELISA) for monitoring food safety. Lateral flow immunoassay is one of the most used point-of-need devices for clinical, environmental, and food safety applications. Compared to traditional methods, it appears to be a simple and fast alternative for detecting food allergens. However, its reliability is frequently questioned due to the lack of quantitative information. In this study, a lateral flow microimmunoassay (LFµIA) is presented that integrates up to 36 spots in microarray format in a single strip, providing semi-quantitative information about the level of allergens, positive and negative controls, internal calibration, and hook effect. The LFµIA has been evaluated for the on-site simultaneous and reliable quantification of almond and peanut allergens as a proof of concept, demonstrating high sensitivity (185 and 229 µg/kg, respectively), selectivity (77%), and accuracy (RSD 5–25%) when analyzing commercial allergen-suspicious food consumables.

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On-chip immunomagnetic separation of allergens from myofibrillar proteins of seafoods for rapid allergy tests

Abstract: The main objective of the current study is to establish an on-chip strategy to analyze the allergens existing in myofibrillar proteins of seafood matrices. On-chip immunomagnetic separation (IMS) of allergens...

Cited by 3 publications

References 38 publications

Food allergen analysis: A review of current gaps and the potential to fill them by matrix‐assisted laser desorption/ionization

Food allergen analysis: A review of current gaps and the potential to fill them by matrix‐assisted laser desorption/ionization

On-Chip Discovery of Allergens from the Exudate of Large Yellow Croaker (Larimichthys Crocea) Muscle Food by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Lateral Flow Microimmunoassay (LFµIA) for the Reliable Quantification of Allergen Traces in Food Consumables

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