On-body hypoxia monitor based on lactate biosensors with a tunable concentration range

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

“…Finally, Table 1 compares the analytical performance of our biosensor with the most relevant electrochemical enzymatic biosensors for L-lactate reported in the last years. The detection limit obtained using GCE/MWCNT-Av/bLOx is comparable to the one reported in [38], lower than the ones reported in [33][34][35]39,40,55,56], and higher than those proposed in [36,37,41,42]. However, the biosensors with detection limits lower than our biosensor presented more complexes schemes for preparation.…”

“…This biosensor was assembled on carbon paper using chitosan as an immobilizing enzymatic agent and Prussian Blue as a mediator for its known electrochemical behavior towards H 2 O 2 . Daboss et al [39] described a sensitive L-lactate biosensor based on screen-printed carbon electrodes modified with Prussian Blue, LOx immobilized in APTES, and with an outer perfluorosulfonated ionomer (Nafion analogue) membrane that acts as a diffusion barrier due to its intrinsic negative charge repulsing negatively charged lactate. The developed biosensor covered the range of physiological L-lactate levels in human sweat and allowed the non-invasive monitoring of hypoxia.…”