On Axenic Cultures of Certain Protozoan and Worm Parasites of Insects*

Jackson, G. J.

doi:10.1111/j.2164-0947.1962.tb01455.x

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Lipid droplets were only infrequently encountered in normal cells cultured at 22 C. Within 2.5 hr after transfer to 37 C, lipid inclusions (Figs. 15 and 16) became noticeably more numerous in the cell population. These inclusions had the typical structure of lipid droplets, although perhaps smaller than those occurring in normal cells.…”

Section: Electron Microscopymentioning

confidence: 94%

Alterations in Fatty Acid Metabolism and Morphology of Leishmania enriettii Exposed to Elevated Temperature

Greenblatt

Wetzel

1966

The Journal of Protozoology

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SYNOPSIS. Cytoplasmic inclusions appear rapidly in Leishmania enriettii exposed to 37 C. The staining of these droplets with oil red O, their extraction in non‐polar solvents, and their fine structure by electron microscopy establish their identity as lipid droplets. Fatty acid profiles of these organisms show alterations concomitant with the development of these inclusions. Oleic acid increases while linolenic acid is depressed in cells exposed to elevated temperature. A greater incorporation of exogenous radioactive stearic acid occurs, with depressed specific activities of linoleic and linolenic acids compared to values obtained with control organisms. The isolated lipids of L. enriettii have temperature‐dependent changes consistent with a physical interpretation of events which occur with temperature inactivation. The temperature lability of fatty acid metabolism is discussed in terms of the synthesis and stability of cellular membranes and organelles.

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Section: Electron Microscopymentioning

confidence: 94%

Alterations in Fatty Acid Metabolism and Morphology of Leishmania enriettii Exposed to Elevated Temperature

Greenblatt

Wetzel

1966

The Journal of Protozoology

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“…Blood for serum was drawn a month or more after the first injection and within a week after the last. Control sera were from uninjected rabbits or from rabbits injected with leptomonads of Leishmania enriettii Muniz & Medina, 1948, cultivated on a blood agar medium (Jackson, 19626).…”

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confidence: 99%

Differentiation of three species ofNeoaplectan(Nematoda: Rhabditida), grown axenically

Jackson

1965

Parasitology

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Three species of the genus Neoaplectana, nematodes from insects, when grown axenically, could be differentiated consistently by morphological, cultivational and serological criteria.All three species were cultivated for consecutive generations on solid media such as a rabbit kidney slice at the bottom of an enriched agar slant. In axenic cultures with various liquid media, including some which were chemically defined, the ensheathed larvae of N. glaseri matured and produced offspring which in turn developed and reproduced; N. dutkii (‘DD-136’) larvae matured, but a new generation did not develop; the majority of N. carpocapsae larvae survived but did not mature. Living N. glaseri, used as a source of antigens to detect homologous antibodies, gave fewer cross-reactions than preparations of sonicated and frozen worms, and fewer false negative reactions than used cultivation media.Morphological differences between the three species in cultures are described.This work was done at The Rockefeller Institute in the department of Dr William Trager, with the technical assistance of June I. Schloemer and, subsequently, Kristin L. Schmidtchen. Preliminary morphological descriptions were made in association with M. B. H. Chitwood of the U.S. Department of Agriculture's Beltsville Parasitological Laboratory in Maryland. The author and work were supported by grants from the U.S. National Institutes of Health, 5-K3-AI-9522 and AI-04842.

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Nutritional Control of Nematode Development

Jackson

1969

Germ-Free Biology Experimental and Clinical Aspects

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On Axenic Cultures of Certain Protozoan and Worm Parasites of Insects*

Cited by 8 publications

References 17 publications

Alterations in Fatty Acid Metabolism and Morphology of Leishmania enriettii Exposed to Elevated Temperature

Alterations in Fatty Acid Metabolism and Morphology of Leishmania enriettii Exposed to Elevated Temperature

Differentiation of three species ofNeoaplectan(Nematoda: Rhabditida), grown axenically

Nutritional Control of Nematode Development

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