On approximation and experimental accuracy in dynamic light scattering

Abstract

The work is dedicated to estimation of accuracy of calculations in dynamic light scattering experiments, in particular approximation and size determination of nanoparticles in polydisperse solutions. In dynamic light scattering the proper approximation is crucial to final accuracy of sizes calculations, especially if we consider polydisperse solutions, such as biological fluids. Calculated and experimental errors have been compared.

Molecular Aggregation in Immune System Activation Studied by Dynamic Light Scattering

“…The absorption spectrum of blood serum was studied in [32,33]. The circuit elements selected according to calculated requirements provided signal-to-noise ratio (SNR) of more than 10 for all detected signals [28]. SNR was calculated using [34][35][36].…”

“…In the commercial DLS spectrometers, the maximum number of components correctly resolved by the algorithm does not exceed 3. The testing of the algorithm and the used spectrometer is described in [28]. It was shown that the error in determining the size of structures in a liquid is less than 8%.…”

“…We compared the results with CONTIN algorithm results, which are used in commercial DLS spectrometers. The developed algorithm proved a significant advantage when the number of components in the studied fluid is more than 3 [28].…”

“…In previous studies, it was demonstrated that the resolution of DLS is extremely large and allows us to measure particle sizes from 0.5 nm to several microns [28]. The size of the investigated molecular structures in the blood usually lies in the range of 1-500 nm.…”