On a Mycoplasma Inhibitor Obtained from Disrupted Mycoplasma Cells: Isolation and some Biological Properties

Altucci, P; Varone, G.L.; Catalano, Gianfranco

doi:10.1159/000220635

Cited by 7 publications

(1 citation statement)

References 2 publications

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“…The reasons for some of the organisms remaining viable for much longer, in some cases for more than 4 months, in the supplemented serum-free media than in the standard medium are not clear. Altucci et al (1969) considered that the toxicity of the metabolic by-products of growth was the reason for the relatively short life-span of mycoplasmas in culture. In our experiments, the maximum titres attained in the supplemented media were usually ten-fold less than in medium with horse serum, so that the toxic effect of metabolic products would probably be reduced and this might account for longer survival.…”

Section: Discussionmentioning

confidence: 99%

The prolonged persistence of mycoplasmas in culture

Taylor‐Robinson¹,

Behnke²

1987

Journal of Medical Microbiology

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Summary. Mycoplasma fermentans organisms in medium containing 20% horse serum multiplied to 107-101* ccu/ml within 5 days at 37°C and were dead usually after 9 days. There was no growth in medium without serum, nor in such medium with the addition of cholesterol or palmitic acid or both, but in some experiments addition of bovine plasma albumin (BPA) increased the number of organisms by up to 1000-fold and some remained viable for up to 84 days. BPA and cholesterol or BPA, palmitic acid and cholesterol more often enhanced growth, in terms of the maximum number of organisms and their survival, than did the addition of BPA alone. The maximum number of organisms in such supplemented serum-free media was usually at least ten-fold less than in medium with horse serum, but some organisms remained viable for up to 13 1 days. Survival of Ureaplasma ureazyticum was also longer in the supplemented serumfree medium than in standard horse-serum medium. The possible factors affecting persistence of mycoplasmas in culture are discussed in relation to these observations.

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Section: Discussionmentioning

confidence: 99%