On a heavy path – determining cold plasma-derived short-lived species chemistry using isotopic labelling

Wende, Kristian; Bruno, Giuliana; Lalk, Michael; Weltmann, Klaus‐Dieter; Woedtke, Thomas von; Bekeschus, Sander; Lackmann, Jan-Wilm

doi:10.1039/c9ra08745a

Cited by 33 publications

(35 citation statements)

References 101 publications

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“…Nevertheless, using adapted conditions for the kINPen or plasma devices generating sufficient amount of atomic oxygen also in open air (e.g. COST-jet) would allow the interaction of atomic oxygen with targets in the bulk liquid as was shown by Benedikt et al 88 or Wende et al 89 . Another plasma source also operated with helium as feed gas, similar to the COST-jet, operated in controlled environment produced mainly atomic oxygen under the investigated conditions reported by Elg et al 90 .…”

Section: Resultsmentioning

confidence: 99%

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

Ravandeh

Kahlert

Jablonowski

et al. 2020

Sci Rep

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Reactive oxygen and nitrogen species (RONS), e.g. generated by cold physical plasma (CPP) or photodynamic therapy, interfere with redox signaling pathways of mammalian cells, inducing downstream consequences spanning from migratory impairment to apoptotic cell death. However, the more austere impact of RONS on cancer cells remains yet to be clarified. In the present study, a combination of electrochemistry and high-resolution mass spectrometry was developed to investigate the resilience of solid-supported lipid bilayers towards plasma-derived reactive species in dependence of their composition. A 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayer was undisturbed by 200 µM H2O2 (control) but showed full permeability after CPP treatment and space-occupying oxidation products such as PoxnoPC, PAzePC, and POPC hydroperoxide were found. Electron paramagnetic resonance spectroscopy demonstrated the presence of hydroxyl radicals and superoxide anion/hydroperoxyl radicals during the treatment. In contrast, small amounts of the intramembrane antioxidant coenzyme Q10 protected the bilayer to 50% and LysoPC was the only POPC derivative found, confirming the membrane protective effect of Q10. Such, the lipid membrane composition including the presence of antioxidants determines the impact of pro-oxidant signals. Given the differences in membrane composition of cancer and healthy cells, this supports the application of cold physical plasma for cancer treatment. In addition, the developed model using the combination of electrochemistry and mass spectrometry could be a promising method to study the effect of reactive species or mixes thereof generated by chemical or physical sources.

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Section: Resultsmentioning

confidence: 99%

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

Ravandeh

Kahlert

Jablonowski

et al. 2020

Sci Rep

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“…Using admixtures of molecular gases, more than 50% of tyrosine was converted into hydroxytyrosine. Target water ionization, homolysis, or photolysis is promoted by excited states of Ar 2 or N 2 (excimers), as well as from radical reactions [ 36 , 90 ]. The direct reaction with those species (or for excimers, with their radiation) leads to the predominant formation in water of ˙OH and ˙H [ 91 , 92 , 93 ], which would react directly with tyrosine to form hydroxytyrosine.…”

Section: Resultsmentioning

confidence: 99%

“…The peak areas of each observed derivative were normalized on the peak area of the control (untreated tyrosine), giving a relative estimate of the conversion. For treatments involving isotopes, the measurement data were corrected according to [ 36 ], taking into account the natural distribution of isotopes and the purity grade.…”

Section: Methodsmentioning

confidence: 99%

“…Gas plasmas can be tuned to create high levels of oxygen species, such as singlet oxygen 1 O 2 ; atomic oxygen ˙O; ozone O 3 ; and, to a lesser extent, hydrogen peroxide H 2 O 2 and hydroxyl radicals ˙OH. These conditions are applied for disinfection or cancer regression [ 36 , 37 , 38 , 39 , 40 ]. In contrast, other conditions foster an N 2 -driven chemistry, yielding species like ˙NO, ˙NO 2 , N 3 O 5 , HNO 2 , and HNO 3 [ 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

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On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

et al. 2020

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Cold physical plasmas modulate cellular redox signaling processes, leading to the evolution of a number of clinical applications in recent years. They are a source of small reactive species, including reactive nitrogen species (RNS). Wound healing is a major application and, as its physiology involves RNS signaling, a correlation between clinical effectiveness and the activity of plasma-derived RNS seems evident. To investigate the type and reactivity of plasma-derived RNS in aqueous systems, a model with tyrosine as a tracer was utilized. By high-resolution mass spectrometry, 26 different tyrosine derivatives including the physiologic nitrotyrosine were identified. The product pattern was distinctive in terms of plasma parameters, especially gas phase composition. By scavenger experiments and isotopic labelling, gaseous nitric dioxide radicals and liquid phase peroxynitrite ions were determined as dominant RNS. The presence of water molecules in the active plasma favored the generation of peroxynitrite. A pilot study, identifying RNS driven post-translational modifications of proteins in healing human wounds after the treatment with cold plasma (kINPen), demonstrated the presence of in vitro determined chemical pathways. The plasma-driven nitration and nitrosylation of tyrosine allows the conclusion that covalent modification of biomolecules by RNS contributes to the clinically observed impact of cold plasmas.

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“…DBDs are known to generate a wide range of reactive species, such as ozone or nitric oxide 24 – 26 , depending on the exact conditions 27 , 28 . A standardized DBD was used in this work, which was already extensively characterised by us in previous studies regarding its plasma parameters, such as the electron density or the gas temperature, and the generated concentration of reactive chemical species 29 , 30 .…”

Section: Introductionmentioning

confidence: 99%

Catalytic oxidation of small organic molecules by cold plasma in solution in the presence of molecular iron complexes†

Śmiłowicz

Kogelheide

Schöne

et al. 2020

Sci Rep

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The plasma-mediated decomposition of volatile organic compounds has previously been investigated in the gas phase with metal oxides as heterogeneous catalysts. While the reactive species in plasma itself are well investigated, very little is known about the influence of metal catalysts in solution. Here, we present initial investigations on the time-dependent plasma-supported oxidation of benzyl alcohol, benzaldehyde and phenol in the presence of molecular iron complexes in solution. Products were identified by HPLC, ESI-MS, FT-IR, and $$^{1}\hbox {H NMR}$$ 1 H NMR spectroscopy. Compared to metal-free oxidation of the substrates, which is caused by reactive oxygen species and leads to a mixture of products, the metal-mediated reactions lead to one product cleanly, and faster than in the metal-free reactions. Most noteworthy, even catalytic amounts of metal complexes induce these clean transformations. The findings described here bear important implications for plasma-supported industrial waste transformations, as well as for plasma-mediated applications in biomedicine, given the fact that iron is the most abundant redox-active metal in the human body.

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On a heavy path – determining cold plasma-derived short-lived species chemistry using isotopic labelling

Abstract: Cold atmospheric plasmas (CAPs) are promising medical tools producing short-lived reactive species.

Cited by 33 publications

References 101 publications

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers

On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

Catalytic oxidation of small organic molecules by cold plasma in solution in the presence of molecular iron complexes†

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