The signless Laplacian matrix of a graph G is given by Q(G) = D(G) + A(G), where D(G) is a diagonal matrix of vertex degrees and A(G) is the adjacency matrix. The largest eigenvalue of Q(G) is called the signless Laplacian spectral radius, denoted by q 1 = q 1 (G). In this paper, some properties between the signless Laplacian spectral radius and perfect matching in graphs are establish. Let r(n) be the largest root of equation x 3 − (3n − 7)x 2 + n(2n − 7)x − 2(n 2 − 7n + 12) = 0. We show that G has a perfect matching for n = 4 or n ≥ 10, if q 1 (G) > r(n), and for n = 6 or n = 8, if q 1 (G) > 4 + 2 √ 3 or q 1 (G) > 6 + 2 √ 6 respectively, where n is a positive even integer number. Moreover, there exists graphs√ 3 and a graph K 3 ∨ K 5 such that q 1 (K 3 ∨ K 5 ) = 6 + 2 √ 6. These graphs all have no prefect matching.