OmpA of a septicemic Escherichia coli O78 – secretion and convergent evolution

Gophna, Uri; Ideses, Diana; Rosen, Ran; Grundland, Adam; Ron, Eliora Z.

doi:10.1016/j.ijmm.2004.08.004

Cited by 21 publications

(23 citation statements)

References 31 publications

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“…Gophna et al (2004) and Power et al (2006) each observed many of the individual polymorphisms reported here. These previous studies linked OmpA polymorphisms to resistance to neutrophil elastase (Gophna et al, 2004) and bacteriophage (Gophna et al, 2004), but did not consider attachment. Our consideration of 78 strains enabled us to identify low-frequency sequence variations that have not been previously reported.…”

Section: Discussionsupporting

confidence: 68%

“…Many of these variations have been previously described, mostly notably in two studies described below (Gophna et al, 2004; Power et al, 2006). To the best of our knowledge, we are the first to report the D variant at position 25 and the polymorphism at position 129.…”

Section: Resultsmentioning

confidence: 78%

“…While some of these polymorphisms have been previously described (Gophna et al, 2004; Power et al, 2006), both of these previous reports only considered a few E. coli strains or only considered two possible ompA alleles. Here we have expanded the list of possible patterns (Table 3A) and considered the co-occurrence of the various polymorphisms (Table 4).…”

Section: Resultsmentioning

confidence: 99%

“…Gophna et al's (2004) analysis identified the outer loop polymorphisms that we have designated pattern I in E. coli strains MG1655, O157:H7 EDL933, and K1 and pattern III in septicemic O78 strain 789 and uropathogenic strain CFT073 (Gophna et al, 2004). Power et al compared the OmpA sequence of three E. coli isolates to the standard MG1655 sequence (Power et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

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Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

et al. 2017

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Understanding the genetic factors that govern microbe-sediment interactions in aquatic environments is important for water quality management and reduction of waterborne disease outbreaks. Although chemical properties of bacteria have been identified that contribute to initiation of attachment, the outer membrane proteins that contribute to these chemical properties still remain unclear. In this study we explored the attachment of 78 Escherichia coli environmental isolates to corn stover, a representative agricultural residue. Outer membrane proteome analysis led to the observation of amino acid variations, some of which had not been previously described, in outer membrane protein A (OmpA) at 10 distinct locations, including each of the four extracellular loops, three of the eight transmembrane segments, the proline-rich linker and the dimerization domain. Some of the polymorphisms within loops 1, 2, and 3 were found to significantly co-occur. Grouping of sequences according to the outer loop polymorphisms revealed five distinct patterns that each occur in at least 5% of our isolates. The two most common patterns, I and II, are encoded by 33.3 and 20.5% of these isolates and differ at each of the four loops. Statistically significant differences in attachment to corn stover were observed among isolates expressing different versions of OmpA and when different versions of OmpA were expressed in the same genetic background. Most notable was the increased corn stover attachment associated with a loop 3 sequence of SNFDGKN relative to the standard SNVYGKN sequence. These results provide further insight into the allelic variation of OmpA and implicate OmpA in contributing to attachment to corn stover.

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Section: Discussionsupporting

confidence: 68%

Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

et al. 2017

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“…An interesting observation is the secretion of proteins, including outer membrane proteins (Gophna et al, 2004). The secretion of OmpA by a septicemic E. coli O78 strain may play a role in virulence, as OmpA has been shown to bind neutrophil elastase, which is bactericidal.…”

Section: Secretion Systemsmentioning

confidence: 99%