OMIP‐048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry

Jaracz‐Ros, Agnieszka; Hémon, Patrice; Krzysiek, Roman; Bachelerie, Françoise; Schlecht-Louf, Géraldine; Gary-Gouy, Hélène

doi:10.1002/cyto.a.23504

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…This allows others to analyze published data by alternative methods and better understand the published material. In the following manuscripts, you can find examples for MIFlowCyt checklists with different MIFlowCyt score values and original FCS data in the FlowRepository for Flow and mass cytometry . Since October 2018 MIFlowCyt compliance and reposition of original data are mandatory for Cytometry Part A publications .…”

Section: Data Handling Evaluation Storage and Repositoriesmentioning

confidence: 99%

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

et al. 2019

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These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.

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Section: Data Handling Evaluation Storage and Repositoriesmentioning

confidence: 99%

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

et al. 2019

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“…As mentioned previously, mass cytometry is also capable of assessing similarly high numbers of parameters. Currently, this technology has the advantage of additional detection channels to accommodate bar coding schemes for sample pooling, and as a more mature technology, high complexity panels using mass cytometry have been previously published and are widely available, including the publication of multiple OMIPs (21)(22)(23)(24). However, limitations such as sample throughput, cell transmission efficiency, and overall cost of ownership have impacted the practicality, and broader adoption, of this technology in some laboratory environments (25)(26)(27).…”

mentioning

confidence: 99%

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

Park¹,

Lannigan²,

Jaimes³

2020

Cytometry Pt A

215

238

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This 40-color flow cytometry-based panel was developed for in-depth immunophenotyping of the major cell subsets present in human peripheral blood. Sample availability can often be limited, especially in cases of clinical trial material, when multiple types of testing are required from a single sample or timepoint. Maximizing the amount of information that can be obtained from a single sample not only provides more in-depth characterization of the immune system but also serves to address the issue of limited sample availability. The panel presented here identifies CD4 T cells, CD8 T cells, regulatory T cells, γδ T cells, NKT-like cells, B cells, NK cells, monocytes and dendritic cells. For each specific cell type, the panel includes markers for further characterization by including a selection of activation and differentiation markers, as well as chemokine receptors. Moreover, the combination of multiple markers in one tube might lead to the discovery of new immune phenotypes and their relevance in certain diseases. Of note, this panel was designed to include only surface markers to avoid the need for fixation and permeabilization steps. The panel can be used for studies aimed at characterizing the immune response in the context of infectious or autoimmune diseases, monitoring cancer patients on immuno-or chemotherapy, and discovery of unique and targetable biomarkers. Different from all previously published OMIPs, this panel was developed using a full spectrum flow cytometer, a technology that has allowed the effective use of 40 fluorescent markers in a single panel. The panel was developed using cryopreserved human peripheral blood mononuclear cells (PBMC) from healthy adults (Table 1). Although we have not tested the panel on fresh PBMCs or whole blood, it is anticipated that the panel could be used in those sample preparations without further optimization.

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“…Among the 70 published OMIPs, 66 (94.3%) are using flow cytometry (either conventional or full-spectrum flow cytometry), and 4 (5.7%) are using mass cytometry (CyTOF) [13][14][15][16]. Most panels focus on human cells (81.4%), including one panel used for humanized mouse [17].…”

Section: Omip Panel Featuresmentioning

confidence: 99%

Orchestrating multiplexity in polychromatic science through OMIPs: A decade‐old resource to empower biomedical research

Wang

Creusot

2021

Cytometry Pt A

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As the optimized multicolor immunofluorescence panel (OMIP) platform entered its 10th anniversary since its launch, the multicolor flow cytometry landscape has changed significantly. Likewise, OMIPs have continuously evolved to cover larger panel sizes, increasing number of subpopulations profiled in a single panel, and new species. After a decade of contributions to the OMIP platform, a review of this collection, summarizing its content and purpose for the research community, is timely and due. This review provides an overview of OMIPs and a presentation of the depth and diversity of this collection of validated panels, with the expectation that readers will take advantage of them to empower and accelerate their research.

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OMIP‐048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry

Cited by 5 publications

References 18 publications

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

Orchestrating multiplexity in polychromatic science through OMIPs: A decade‐old resource to empower biomedical research

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