OMIP‐012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice

Abstract

PURPOSE AND APPROPRIATE SAMPLE TYPESThis panel was developed to determine both the frequency and absolute number of human leukocytes and leukocyte subsets present in the peripheral blood of humanized mice. The panel also provides information concerning the activation state of peripheral leukocytes by cell surface staining for HLA-DR and CD38, relevant to studies of HIV disease pathogenesis (1-3). This panel has been used with EDTA anticoagulated whole blood in conjunction with bead-based enumeration for quantitative assessment of human cell chimerism. This panel also works well for staining of peripheral blood mononuclear cells (PBMCs) prepared by density gradient centrifugation and for dispersed splenocytes. The multicolor panel described here has been used in studies of humanized mouse reconstitution (4) and longitudinal studies of HIV pathogenesis in NSG-BLT mice (5). BACKGROUND

OMIP‐023: 10‐Color, 13 antibody panel for in‐depth phenotyping of human peripheral blood leukocytes

“…This panel can be used for in-depth phenotyping of peripheral blood samples as well as cord blood in one tube. The panel is in a manner similar to OMIP-001, 002, 004, 007, 010, 012, 015, and 018 (3)(4)(5)(8)(9)(10)(11)(12).…”

Orchestrating multiplexity in polychromatic science through OMIPs: A decade‐old resource to empower biomedical research

“…Among the 70 published OMIPs, 66 (94.3%) are using flow cytometry (either conventional or full-spectrum flow cytometry), and 4 (5.7%) are using mass cytometry (CyTOF) [13][14][15][16]. Most panels focus on human cells (81.4%), including one panel used for humanized mouse [17]. Recently, the species covered by OMIPs have expanded to rat (OMIP-041), mouse (OMIP-031, 032, 048, 054, 057, 059, 061), nonhuman primates (OMIP-005, 016, 026, 028, 035, 052), and dog (OMIP-065) (Figure 3(A) and Table S1).…”