Omics strategies decipher therapeutic discoveries of traditional Chinese medicine against different diseases at multiple layers molecular-level

Guo, Rui; Luo, Xialin; Liu, Jingjing; Liu, Lian; Wang, Xijun; Lü, Haitao

doi:10.1016/j.phrs.2020.104627

Cited by 73 publications

(42 citation statements)

References 62 publications

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“…And this is not conducive to the further research and utilization of medicinal value of loquat. In recent years, traditional Chinese medicine (TCM) has been greatly accelerated by the analytical technologies and methodologies of genomics, proteomics and metabonomics [53,54] . Among these Omics, metabolomics is a very valuable for the analysis of components including various metabolites of TCM [55] .…”

Section: Discussionmentioning

confidence: 99%

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)

Wang¹

2021

Preprint

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Eriobotrya japonica, commonly called loquat, is a type of fruit and a famous traditional Chinese medicinal material. Here, a high-quality draft genome of the E. japonica cultivar, Big Five-pointed Star, containing 733.32 million bases (Mb) covering approximately 98% of estimated whole genome size (749.25 Mb) is reported. A total of 45,492 protein-coding genes were predicted. Meanwhile, metabolomes of flower, leave and root of this loquat variety were also determined by UPLC-ESI-MS/M system. 577 metabolites were determined in total, including 98 phenolic acids, 95 flavonoids, 33 alkaloids, 28 terpenoids, one steroids. The accumulation difference of these metabolites among leaf, flower and root of loquat were also clarified. Based on KEGG annotation, genes related to the biosynthesis of medicinal ingredients including some flavonoids and terpenoids were identified. Overall, this study provides global fundamental molecular insights into the medical biology of Eriobotrya japonica.

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Section: Discussionmentioning

confidence: 99%

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)

Wang¹

2021

Preprint

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“…For many years, RNA-seq has become an essential tool for studying the dynamic and complex characteristics of the transcriptome, especially for the mRNA molecules, which encode proteins following the central dogma of molecular biology [25,26]. Meanwhile, many scientists in the research eld of Chinese medicine have paid more and more attention to explore the molecular control of Chinese herbs and formulations by taking advantage of RNA-seq technology [27,28]. Our previous studies have shown that DAE play potential roles in regulating chondrocyte proliferation and differentiation [15,16].…”

Section: Discussionmentioning

confidence: 99%

Insights Into the Molecular Mechanism of Deer Antler Extract Serving as a Potential Chondroprotective Agent for Articular Cartilage

Yao¹,

Zhou²,

Zhang³

et al. 2020

Preprint

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Background Deer antler is considered as a precious traditional Chinese medicinal material, and has been widely used to reinforce kidney’s yang, nourish essence and strengthen bone function. The most prominent bioactive components in deer antler are water-soluble proteins that play potential roles in bone formation and repair. The aim of this study was to explore the molecular control and therapeutic targets of deer antler extract (DAE) on articular cartilage. Methods DAE was prepared as previously described. All rats were randomly divided into Blank group and DAE group (10 rats per group) after 7-day adaptive feeding. The rats in DAE group were orally administrated with DAE at a dose of 0.2 g/kg per day for 3 weeks and the rats in Blank group were fed with drinking water. Total RNA was isolated from the articular cartilage of knee joints. RNA sequencing (RNA-seq) experiment combined with quantitative real-time polymerase chain reaction (qRT-PCR) verification assay were carried out to explore the molecular control and therapeutic targets of DAE on articular cartilage. Results We demonstrated that DAE played a potential role in promoting cartilage formation, growth and repair, and inhibiting cartilage degeneration and inflammation. These effects were possibly achieved by accelerating the expression of functional genes involved in chondrocyte commitment, survival, proliferation and differentiation, and suppressing the expression of susceptibility genes involved in the pathophysiology of osteoarthritis. Conclusions DAE might serve as a chondroprotective agent for treating cartilage degeneration and inflammation by boosting the ability of cartilage growth and repair. Thus, our findings will contribute towards deepening the knowledge about the molecular control and therapeutic targets of DAE on the treatment of osteoarthritis.

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“…TCM holds the unique advantages of multi-target and multi-pathway regulation in the treatment of complex diseases with high e cacy, low toxicity, and few side effects [4][5][6][7][8][9] . Previous research illustrated that RJSJ is safe and well tolerated, and it does not induce body weight loss, immune dysregulation, or myelosuppression in mice 13 .…”

Section: Discussionmentioning

confidence: 99%

“…The characteristics and advantages of traditional Chinese medicine (TCM) in the prevention and treatment of complex diseases, such as cancers, cardiovascular diseases, infectious diseases, metabolic diseases, and neurodegenerative diseases, have attracted global attention, and such treatments have been illustrated to ameliorate symptoms, improve patient quality of life, and prolong survival [4][5][6][7][8][9] . In 2018, the WHO International Classi cation of Diseases listed traditional medicines, including TCM, in its classi cation system for the rst time 10 .…”

Section: Introductionmentioning

confidence: 99%

Ruanjian Sanjie Decoction Induces Apoptosis in Triple-negative Breast Cancer Cells by Downregulating cIAP1/2 and XIAP

Zhao¹,

Wang²,

Jia³

et al. 2020

Preprint

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Background: Traditional Chinese medicine (TCM) comprises a unique theoretical system developed over thousands of years. The previous study reported that Ruanjian Sanjie (RJSJ) exerts anti-tumor effects by inducing cell apoptosis. However, the mechanism is not clear. Methods: In this study, we investigated the possible mechanism by the strategy of combining network pharmacology analysis with experiment (in vitro and in vivo). First, four kinds of breast cancer cell lines were used to conduct proliferation, apoptosis and cell cycle analysis. Secondly, to study pathophysiological processes of breast cancer at the molecular network level, we for the first time constructed an “integrated apoptosis module network of breast cancer” by assembling the regulatory relationships of canonical apoptosis signaling pathways. Through the strategy of combining network analysis and experiments, we analyzed the main mechanism of RJSJ in breast cancer and screened out the core genes. We further studied the inhibitory effect of RJSJ combined with carboplatin (CBP) in vivo. Finally, the synergistic effect of RJSJ and CBP were analyzed and the potential active components in RJSJ were predicted.Results: This study demonstrated that RJSJ could significantly inhibit breast cancer cell proliferation and induce apoptosis in a concentration-dependent manner. The primary mechanism of RJSJ in the treatment of breast cancer was pro-apoptotic. The core apoptosis genes regulated by RJSJ were cIAP1/2 and XIAP. We also found that RJSJ in combination with CBP tended to synergistically induce apoptosis, which might mainly be achieved through the regulation of multiple targets and pathways. Alexandrin (BX05, XKC02, SCG01), baicalin (BX22), guanosine (BX32), arjunglucoside I (XKC10) etc. were predicted as potential active components.Conclusions: These findings provide the rationale for exploring the therapeutic effects of RJSJ against breast cancer and providing a bridge for the combined use of Chinese and Western medicine.

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Omics strategies decipher therapeutic discoveries of traditional Chinese medicine against different diseases at multiple layers molecular-level

Cited by 73 publications

References 62 publications

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)

Insights Into the Molecular Mechanism of Deer Antler Extract Serving as a Potential Chondroprotective Agent for Articular Cartilage

Ruanjian Sanjie Decoction Induces Apoptosis in Triple-negative Breast Cancer Cells by Downregulating cIAP1/2 and XIAP

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Omics strategies decipher therapeutic discoveries of traditional Chinese medicine against different diseases at multiple layers molecular-level

Cited by 73 publications

References 62 publications

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica&nbsp;(Thunb.) Lindl)

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica&nbsp;(Thunb.) Lindl)

Insights Into the Molecular Mechanism of Deer Antler Extract Serving as a Potential Chondroprotective Agent for Articular Cartilage

Ruanjian Sanjie Decoction Induces Apoptosis in Triple-negative Breast Cancer Cells by Downregulating cIAP1/2 and XIAP

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A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)

A draft genome at chromosome level and metabolomes of leave, root and flowers provide insights into the molecular basis of medicinal ingredients of loquat (Eriobotrya japonica (Thunb.) Lindl)