Omics‐based molecular analyses of adhesion by aquatic invertebrates

Davey, Peter A.; Power, Anne; Santos, Romana; Bertemes, Philip; Ladurner, Peter; Palmowski, Paweł; Clarke, Jessica L.; Flammang, Patrick; Lengerer, Birgit; Hennebert, Elise; Rothbächer, Ute; Pjeta, Robert; Wunderer, Julia; Z̆urovec, Michal; Aldred, Nick

doi:10.1111/brv.12691

Cited by 33 publications

(46 citation statements)

References 197 publications

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“…The core region of Mlig-ap2 is composed of two highly repetitive protein motifs (RP-1 and RP-2) [13]. Likewise, Mpoz-ap2 was assembled with two repeat motifs in the centre of the protein [25]. However, short-readbased transcriptome assembly was not able to fully assemble the long ap2, including repeat regions 1 and 2, in the other species.…”

Section: Adhesion Proteinmentioning

confidence: 99%

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(Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms

Bertemes

Pjeta

Wunderer

et al. 2021

IJMS

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Many free-living flatworms have evolved a temporary adhesion system, which allows them to quickly attach to and release from diverse substrates. In the marine Macrostomum lignano, the morphology of the adhesive system and the adhesion-related proteins have been characterised. However, little is known about how temporary adhesion is performed in other aquatic environments. Here, we performed a 3D reconstruction of the M. lignano adhesive organ and compared it to the morphology of five selected Macrostomum, representing two marine, one brackish, and two freshwater species. We compared the protein domains of the two adhesive proteins, as well as an anchor cell-specific intermediate filament. We analysed the gene expression of these proteins by in situ hybridisation and performed functional knockdowns with RNA interference. Remarkably, there are almost no differences in terms of morphology, protein regions, and gene expression based on marine, brackish, and freshwater habitats. This implies that glue components produced by macrostomids are conserved among species, and this set of two-component glue functions from low to high salinity. These findings could contribute to the development of novel reversible biomimetic glues that work in all wet environments and could have applications in drug delivery systems, tissue adhesives, or wound dressings.

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Section: Adhesion Proteinmentioning

confidence: 99%

“…After detachment, footprints consisting of Mlig-ap1 and Mlig-ap2 remain on the substrate. Knowledge on bioadhesive molecules in other Macrostomum species is scarce, but in the freshwater M. poznaniense, a knockdown of the ap2-like gene also impeded attachment [25].…”

Section: Introductionmentioning

confidence: 99%

(Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms

Bertemes

Pjeta

Wunderer

et al. 2021

IJMS

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“…Omics technologies have significantly contributed to the understanding of underwater bioadhesion and have provided a number of putative protein-based biomaterials [ 14 ]. The adhesive proteins of sessile marine organisms with a defined secretory gland, such as mussels and scallops, can be identified through a combination of transcriptomics and proteomics [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Wang

Zhang

et al. 2022

Bioactive Materials

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Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidant materials has limited their further development. Novel bioavailable antioxidant materials that are derived from natural resources are urgently needed. Here, an integrated multi-omics method was applied to fabricate antioxidant biomaterials. A key cysteine-rich thrombospondin-1 type I repeat-like (TSRL) protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L −1 . The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca 2+ -mediated coordination or redox-responsive hydrogels with tunable physical properties. The TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rates than glutathione (GSH) and ascorbic acid (Aa) and protected cells against external oxidative stress significantly more effectively. When topically applied to mice skin, TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B (UVB) irradiation, confirming that it enhanced antioxidant activity in vivo . This is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives, and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.

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“…Mechanical aspects play a role in adhesion, particularly in acorn barnacles where shell geometry [2] and base plate mechanical properties [5,6] are major contributors. While the exact mechanisms of how proteins contribute to barnacle adhesion are unknown, many have been proposed [7][8][9], including specific protein-protein interactions [10][11][12][13], repetitive sequence motifs [14] that drive nanofibril formation [15][16][17][18][19], evolution of novel adhesive functions from ancestral wound healing processes [20] and oxidative modification of proteins [21]. All evidence points to barnacle adhesion being distinct from other well-studied marine adhesive processes which often rely on serine phosphorylation or DOPA [22,23].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of stalked and acorn barnacle adhesive proteomes

et al. 2021

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Barnacles interest the scientific community for multiple reasons: their unique evolutionary trajectory, vast diversity and economic impact—as a harvested food source and also as one of the most prolific macroscopic hard biofouling organisms. A common, yet novel, trait among barnacles is adhesion, which has enabled a sessile adult existence and global colonization of the oceans. Barnacle adhesive is primarily composed of proteins, but knowledge of how the adhesive proteome varies across the tree of life is unknown due to a lack of genomic information. Here, we supplement previous mass spectrometry analyses of barnacle adhesive with recently sequenced genomes to compare the adhesive proteomes of Pollicipes pollicipes (Pedunculata) and Amphibalanus amphitrite (Sessilia). Although both species contain the same broad protein categories, we detail differences that exist between these species. The barnacle-unique cement proteins show the greatest difference between species, although these differences are diminished when amino acid composition and glycosylation potential are considered. By performing an in-depth comparison of the adhesive proteomes of these distantly related barnacle species, we show their similarities and provide a roadmap for future studies examining sequence-specific differences to identify the proteins responsible for functional differences across the barnacle tree of life.

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Omics‐based molecular analyses of adhesion by aquatic invertebrates

Cited by 33 publications

References 197 publications

(Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms

(Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms

Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Comparative analysis of stalked and acorn barnacle adhesive proteomes

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