Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation

Kunc, Martin; Dobeš, Pavel; Ward, Rachel; Lee, Saetbyeol; Čegan, Radim; Dostálková, Silvie; Holušová, Kateřina; Hurychová, Jana; Eliáš, Sara; Pinďáková, Eliška; Čukanová, Eliška; Prodělalová, Jana; Petřivalský, Marek; Danihlík, Jiří; Havlík, Jaroslav; Hobza, Roman; Kavanagh, Kevin; Hyršl, Pavel

doi:10.1016/j.ibmb.2022.103877

Cited by 11 publications

(13 citation statements)

References 104 publications

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“…Overall, our observations summarized in Figure 7 are in agreement with the following previous results on the effect of Varroa-DWV on bees: (1) the levels of lipid metabolism-related proteins increase in parasitized worker pupae [52]; (2) the levels of proteins related to the metabolism of lipids and branched amino acids increase in the hemolymph of Varroa-parasitized bees [14]; and (3) the sphingolipid metabolism rate increases in Varroa-parasitized 10-day-old bees [22].…”

Section: Pathways Affected In Beessupporting

confidence: 92%

“…Overall, the results of this study complement those of other studies that have produced similar or different results and interpretations most likely due to differences in study designs and/or analyses of developmental stages, castes, and ages of the bees [23,24]. For instance, proteomic studies have analyzed the effect of Varroa parasitization at the time of emergence [18], in 10-day-old bees [22], in the hemolymph of 1-day-old bees [14], and in worker and drone pupae [52].…”

Section: Original System For Investigating the Specific Developmental...supporting

confidence: 72%

“…was downregulated [22]. The observed differences in the impact of Varroa mites on bees reported in various studies most likely resulted from differences in experimental designs and the use of different developmental stages and castes of bees [23,24].…”

Section: Significance Statementmentioning

confidence: 97%

“…In addition, suppression of mTORC1 activity has been linked to alterations in cholesterol transport [73], the hijacking (i.e., upregulation of NPC2; LOC724386) of which is essential for infection and the life cycle [74]. Because of the link to the nutrient-sensing pathway, the collective changes in the mTORC1 pathway and peroxisomal β-oxidation [75] may explain the increase in the sphingolipid metabolic rate observed by Kunc et al [22] in 10 day old parasitized bees.…”

Section: Pathways Affected In Beesmentioning

confidence: 98%

“…However, in other studies, only a weak association between Varroa infection and immunosuppression was observed [19–21]. Most recently, a significant upregulation of the immune response of bees to Varroa infection was observed in 10‐day‐old bees from Varroa ‐infested colonies compared with those from control colonies [22]. Specifically, the antimicrobial peptides apidaecin, abaecin, defensin, and hymenoptaecin were strongly upregulated in Varroa ‐parasitized bees.…”

Section: Introductionmentioning

confidence: 95%

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Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome‐induced pathways

Erban,

Kadleckova,

Sopko

et al. 2024

Proteomics

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The ectoparasitic mite Varroa destructor transmits and triggers viral infections that have deleterious effects on honey bee colonies worldwide. We performed a manipulative experiment in which worker bees collected at emergence were exposed to Varroa for 72 h, and their proteomes were compared with those of untreated control bees. Label‐free quantitative proteomics identified 77 differentially expressed A. mellifera proteins (DEPs). In addition, viral proteins were identified by orthogonal analysis, and most importantly, Deformed wing virus (DWV) was found at high levels/intensity in Varroa‐exposed bees. Pathway enrichment analysis suggested that the main pathways affected included peroxisomal metabolism, cyto‐/exoskeleton reorganization, and cuticular proteins. Detailed examination of individual DEPs revealed that additional changes in DEPs were associated with peroxisomal function. In addition, the proteome data support the importance of TGF‐β signaling in Varroa–DWV interaction and the involvement of the mTORC1 and Hippo pathways. These results suggest that the effect of DWV on bees associated with Varroa feeding results in aberrant autophagy. In particular, autophagy is selectively modulated by peroxisomes, to which the observed proteome changes strongly corresponded. This study complements previous research with different study designs and suggests the importance of the peroxisome, which plays a key role in viral infections.

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Section: Pathways Affected In Beessupporting

confidence: 92%

Section: Original System For Investigating the Specific Developmental...supporting

confidence: 72%

Section: Significance Statementmentioning

confidence: 97%

Section: Pathways Affected In Beesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome‐induced pathways

Erban,

Kadleckova,

Sopko

et al. 2024

Proteomics

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What proteomics has taught us about honey bee (Apis mellifera) health and disease

Arad,

Ku,

Frey

et al. 2024

Proteomics

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The Western honey bee, Apis mellifera, is currently navigating a gauntlet of environmental pressures, including the persistent threat of parasites, pathogens, and climate change – all of which compromise the vitality of honey bee colonies. The repercussions of their declining health extend beyond the immediate concerns of apiarists, potentially imposing economic burdens on society through diminished agricultural productivity. Hence, there is an imperative to devise innovative monitoring techniques for assessing the health of honey bee populations. Proteomics, recognized for its proficiency in biomarker identification and protein–protein interactions, is poised to play a pivotal role in this regard. It offers a promising avenue for monitoring and enhancing the resilience of honey bee colonies, thereby contributing to the stability of global food supplies. This review delves into the recent proteomic studies of A. mellifera, highlighting specific proteins of interest and envisioning the potential of proteomics to improve sustainable beekeeping practices amidst the challenges of a changing planet.

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Investigation of resistance against to flumethrin using against Varroa destructor in Türkiye

Yarsan,

Yilmaz,

Sevin

et al. 2024

Vet Res Commun

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The honeybee ectoparasite Varroa destructor is a major threat to apiculture when evaluating bee diseases and pests. While attempting to control this mite, beekeepers often depend on a small selection of authorized synthetic acaricides, such as flumethrin, which is widely used in Türkiye and globally. However, resistance to flumethrin develops due to incorrect and excessive use. In this study conducted at Ordu Beekeeping Research Institute, trial group were established including an untreated control group and group where flumethrin-based pesticides were applied. Dead varroas collected from pollen traps and live varroas collected from bees were obtained from these trial groups for molecular analysis as positive-negative controls. Varroa samples were collected from provinces representing different regions with intensive beekeeping activities such as Adana, Ankara, Bingöl, Muğla, Ordu, Şanlıurfa, Tekirdağ. Molecular methods were employed to investigate the resistance gene region for pyrethroids (specifically flumethrin) against V. destructor. In our study, individual DNA extractions were performed on dead parasites from colonies subjected to pyrethroid application (resistance negative control) and live parasites (resistance positive control). The DNA samples obtained were used in PCR reactions targeting the region encoding the 925th amino acid of the voltage-gated sodium channel (VGSC) gene, which is responsible for resistance formation. The DNA samples were subjected to gel electrophoresis to observe the amplification products of the expected target region. To examine the nucleotide sequence changes that encode leucine at the 925th amino acid, which is associated with resistance, DNA sequence analysis was applied to the amplification products. Out of 332 V. destructor parasites obtained from different provinces, 279 were analysed using molecular methods. It was observed that 31% of the samples showed sensitivity to flumethrin while 69% exhibited resistance to it. Among the resistant samples: 27% had homozygous isoleucine mutation; 28% had homozygous valine mutation; 2.8% had heterozygous isoleucine mutation; 8.5% had heterozygous valine mutation; and 2.8% had heterozygous methionine mutation, all of which were associated with flumethrin resistance. As a result, the rate of flumethrin resistance in parasites varied between 51% and 94% among different provinces.

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Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation

Cited by 11 publications

References 104 publications

Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome‐induced pathways

Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome‐induced pathways

What proteomics has taught us about honey bee (Apis mellifera) health and disease

Investigation of resistance against to flumethrin using against Varroa destructor in Türkiye

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