Omics as a window to view embryo viability

Krisher, Rebecca L.; Schoolcraft, William B.; Katz‐Jaffe, Mandy G.

doi:10.1016/j.fertnstert.2014.12.116

Cited by 57 publications

(43 citation statements)

References 86 publications

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“…Stigliani and colleagues (2013) also found that mitochondrial DNA quantity in culture media is significantly correlated with maternal age and the degree of cellular fragmentation and thus, may be an indication of poor embryo quality and aneuploidy. Lastly, “-omics” profiling of follicular fluid and genetic analysis of the oocyte-surrounding cumulus cells has been investigated for the purpose of pre-implantation screening and is reviewed extensively elsewhere (Assou et al 2010; Seli et al 2010a; Krisher et al 2015). Taken together, we suggest that although these non-invasive aneuploidy detection methods may not serve as a substitute for PGS, especially when used alone, the analysis of a panel of biomarkers in conjunction with other approaches such as cfDNA or TLM analysis may assist in embryo diagnostics.…”

Section: Non-invasive Methods For Aneuploidy Detectionmentioning

confidence: 99%

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Daughtry

Chavez

2015

Cell Tissue Res

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Formation of a totipotent blastocyst capable of implantation is one of the first major milestones in early mammalian embryogenesis, but less than half of in vitro fertilized embryos from most mammals will progress to this stage of development. Whole chromosomal abnormalities, or aneuploidy, are key determinants of whether human embryos will arrest or reach the blastocyst stage. Depending on the type of chromosomal abnormality, however, certain embryos still form blastocysts and may be morphologically indistinguishable from chromosomally normal embryos. Despite the implementation of pre-implantation genetic screening and other advanced in vitro fertilization (IVF) techniques, the identification of aneuploid embryos remains complicated by high rates of mosaicism, atypical cell division, cellular fragmentation, sub-chromosomal instability, and micro-/multi-nucleation. Moreover, several of these processes occur in vivo following natural human conception, suggesting that they are not simply a consequence of culture conditions. Recent technological achievements in genetic, epigenetic, chromosomal, and non-invasive imaging have provided additional embryo assessment approaches, particularly at the single-cell level, and clinical trials investigating their efficacy are continuing to emerge. In this review, we summarize the potential mechanisms by which aneuploidy may arise, the various detection methods, and the technical advances (such as time-lapse imaging, “-omic” profiling, and next-generation sequencing) that have assisted in obtaining this data. We also discuss the possibility of aneuploidy resolution in embryos via various corrective mechanisms, including multi-polar divisions, fragment resorption, endoreduplication, and blastomere exclusion, and conclude by examining the potential implications of these findings for IVF success and human fecundity.

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Section: Non-invasive Methods For Aneuploidy Detectionmentioning

confidence: 99%

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Daughtry

Chavez

2015

Cell Tissue Res

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“…However, theoretical risks from prolonged culture of embryos on epigenetic errors still need to be kept under scrutiny. Third, we should continue to develop non-invasive methods of embryo screening such as proteomics, metabolomics, and examination of oocyte and cumulus-cell gene expression [24][25][26][27]. Time-lapse technology has recently been adopted by several IVF clinics across the USA with some studies showing promising results regarding the technology's ability to screen for healthy embryos that are most likely to implant.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have reported on the use of proteomics and metabolomics to identify factors in embryo culture media that may be predictive of embryo competence or assessing gene expression in cumulus cells; however, even these methods are still inefficient and not ready yet for clinical application [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Embryo wastage rates remain high in assisted reproductive technology (ART): a look at the trends from 2004–2013 in the USA

Ghazal¹,

Patrizio²

2016

J Assist Reprod Genet

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This work examined the trend in Bembryo wastager ates after ART in USA and its relationship to the number of embryos transferred, live born infants delivered across patient age, and the yearly percentage of embryos wasted. The data were obtained from the US-clinics SART databank for the years 2004-2013. A total of 1,808,082 non-donor embryos were transferred in 748,394 fresh cycles resulting in 358,214 liveborn. During the years of analysis, the mean number of embryos transferred has progressively decreased leading to an overall significant decrease in Embryo Wastage rates (83.2 to 76.5%, p < 0.001) while the percentage of transfers leading to a live born increased (24.8 to 27.8%, p = 0.002). Embryo Wastage negatively correlated with percentage of transfers resulting in live birth (p = 0.001), and the average number of embryos transferred positively correlated with the percentage of embryos wasted (p < 0.001). The overwhelming majority of embryos transferred still do not result into a live birth confirming that only few embryos per ART cycle are competent. The overall BEmbryo Wastage^rates have consistently decreased from a high of 90% in 1995 to a rate of 76.5% in 2013. Transferring fewer embryos particularly at the blastocyst-stage and improved methods of embryo selection may further decrease BEmbryo Wastage^rates.

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“…Overlapping depicts the influence each constituent has on one another -the secretome is primarily influenced by uterine glandular and luminal epithelia in addition to conceptus presence, and to a lesser extent, leak-through from the maternal vasculature, whose composition is in turn influenced by maternal physiology. For a comprehensive review on embryo 'omics' please refer to Krisher et al (2015).…”

Section: Secretome Proteomicsmentioning

confidence: 99%

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

Simintiras¹,

Forde²

2017

Anim Reprod

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Early pregnancy loss in cattle can be attributed to a myriad of sources. One key factor that can influence early pregnancy success or loss is the influence and interactions between the maternal environment and the developing embryo/conceptus. Recent advances in high-throughput 'omics' technologies coupled with improved bioinformatics capabilities represent a promising avenue for enhancing our understanding of fundamental developmental events -which would have direct agricultural, veterinary, and economic benefits. Thusly this review revolves around recent applications of advanced transcriptomic, proteomic, and metabolomic analyses within a bovine uterine secretomic and interactomic context, with an overriding aim to highlight the advantages of these emerging fields whilst identifying areas for improvement, consideration, and further research and development.

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Omics as a window to view embryo viability

Cited by 57 publications

References 86 publications

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Embryo wastage rates remain high in assisted reproductive technology (ART): a look at the trends from 2004–2013 in the USA

Understanding the uterine environment in early pregnancy in cattle: How have the omics en-hanced our knowledge?

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