‘Omics’ and Plant Responses to Botrytis cinerea

AbuQamar, Synan F.; Moustafa, Khaled; Tran, Lam‐Son Phan

doi:10.3389/fpls.2016.01658

Cited by 72 publications

(53 citation statements)

References 78 publications

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“…Here, we report the symptoms, the pathogen as well as the proper chemical treatment to manage stem canker as the first step toward planning IDM programs against this devastating disease on royal poinciana in the UAE or elsewhere. In the current study, the phenotype i.e., symptoms associated with the disease can be considered as a starting point for future comparative 'omic' analyses including genomes and responses to environmental variation [47]. A combination of different methods to achieve suitable IDM practices is on top of our priorities.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Raish

Saeed

Sham

et al. 2020

IJMS

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In the United Arab Emirates (UAE), royal poinciana (Delonix regia) trees suffer from stem canker disease. Symptoms of stem canker can be characterized by branch and leaf dryness, bark lesions, discoloration of xylem tissues, longitudinal wood necrosis and extensive gumming. General dieback signs were also observed leading to complete defoliation of leaves and ultimately death of trees in advanced stages. The fungus, Neoscytalidium dimidiatum DSM 109897, was consistently recovered from diseased royal poinciana tissues; this was confirmed by the molecular, structural and morphological studies. Phylogenetic analyses of the translation elongation factor 1-a (TEF1-α) of N. dimidiatum from the UAE with reference specimens of Botryosphaeriaceae family validated the identity of the pathogen. To manage the disease, the chemical fungicides, Protifert®, Cidely® Top and Amistrar® Top, significantly inhibited mycelial growth and reduced conidial numbers of N. dimidiatum in laboratory and greenhouse experiments. The described “apple bioassay” is an innovative approach that can be useful when performing fungicide treatment studies. Under field conditions, Cidely® Top proved to be the most effective fungicide against N. dimidiatum among all tested treatments. Our data suggest that the causal agent of stem canker disease on royal poinciana in the UAE is N. dimidiatum.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Raish

Saeed

Sham

et al. 2020

IJMS

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“…Our microarray analysis demonstrated similar results with other studies. For example, genes that are either considered as JA-responsive or involved in biosynthesis of JA were differentially expressed at 24 hpi in wrky33 mutant and/or 35S:WRKY33 overexpressing lines compared with wild-type (S6 Table) [51,68]. Similarly, genes involved in JA/ET-mediated signaling, SA signaling, camalexin biosynthesis, and redox homeostasis were differentially-regulated by WRKY33 in Arabidopsis plants inoculated with B. cinerea.…”

Section: Validation Of Obigs And/or Pbigs Dependent On Wrky33 To B Cmentioning

confidence: 99%

Microarray analysis of Arabidopsis WRKY33 mutants in response to the necrotrophic fungus Botrytis cinerea

AbuQamar¹,

Moustafa²

2018

Preprint

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The WRKY33 transcription factor was reported for resistance to the necrotrophic fungus Botrytis cinerea. Using microarray-based analysis, we compared Arabidopsis WRKY33 overexpressing lines and wrky33 mutant that showed altered susceptibility to B. cinerea with their corresponding wild-type plants. In the wild-type, about 1660 genes (7% of the transcriptome) were induced and 1054 genes (5% of the transcriptome) were repressed at least twofold at early stages of inoculation with B. cinerea, confirming previous data of the contribution of these genes in B. cinerea resistance. In Arabidopsis wild-type plant infected with B. cinerea, the expressions of the differentially expressed genes encoding for proteins and metabolites involved in pathogen defense and non-defense responses, seem to be dependent on a functional WRKY33 gene. The expression profile of 12-oxo-phytodienoic acidand phytoprostane A 1 -treated Arabidopsis plants in response to B. cinerea revealed that cyclopentenones can also modulate WRKY33 regulation upon inoculation with B. cinerea. These results support the role of electrophilic oxylipins in mediating plant responses to B. cinerea infection through the TGA transcription factor. Future directions toward the identification of the molecular components in cyclopentenone signaling will elucidate the novel oxylipin signal transduction pathways in plant defense.cinerea induces host cell death by producing toxins, cell wall degrading enzymes and reactive oxygen intermediates (ROIs) [1,4,5]. Although cell death and accumulation of ROIs are associated with plant resistance to biotrophic pathogens [6], the ROIs can also increase plant susceptibility to necrotrophs [7]. In addition, the plant polygalacturonase-inhibiting proteins counteract polygalacturonase which are important host colonizing factors for some fungal pathogens [8]. Although the cell wall and cuticle protect plants against pathogen penetration or infection, Arabidopsis mutants defective in components of the cell wall and cuticle were resistant to B. cinerea [9][10][11]. In fact, the cell wall and cuticle are primary barriers against pathogen attacks that may decrease or enhance plant resistance to pathogens. For instance, a lossof-function of the HISTONE MONOUBIQUITINATION1 (HUB1) gene, encoding an E3 ligase required for histone H2B ubiquitination, reduces the cell wall thickness and increases the susceptibility to B. cinerea and Alternaria brassicicola [12].Similarly to animals, plants recognize elicitors derived from pathogens to activate innate immune defense responses [13]. In contrast to race-specific elicitors or resistance genes described for biotroph-plant interactions, plants recognize a pathogen -regardless of its lifestyle-via MAMP that serve as general elicitors [14,15]. Chitins and glucans are fungal MAMPs that plants can recognize by pattern recognition receptors. The Arabidopsis receptor kinases, FLS2 (flagellin sensing 2) and EFR (elongation factor Tu receptor), independently recognize the bacterial flagellin (flg22) and elongati...

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“…The necrotrophic fungal pathogen Botrytis cinerea causes gray mold on more than 200 plant species worldwide, including numerous economically important horticultural crops . Botrytis cinerea can infect all above‐ground plant parts and is most destructive to mature or senescent organs.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

Meng

2018

J Sci Food Agric

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BACKGROUND: The fungal pathogen Botrytis cinerea infects a broad range of horticultural plants worldwide, resulting in significant economic losses. A derivative of chitosan, oligochitosan, has been reported to be an eco-friendly alternative to synthetic fungicides. RESULTS:Oligochitosan can greatly inhibit B. cinerea spore germination and induce protein carbonylation. To further investigate the molecular mechanism underlying the inhibitory effect, a comparative proteome analysis was conducted of oligochitosan-treated versus non-treated B. cinerea spores. The cellular proteins were obtained from B. cinerea spore samples and subjected to two-dimensional gel electrophoresis. In total, 21 differentially expressed proteins (DEPs) were identified. Three DEPs were up-regulated in the oligochitosan-treated versus the untreated spores, including scytalone dehydratase and a serine carboxypeptidase III precursor. By contrast, seven DEPs, including Hsp 88 and cell division cycle protein 48, were down-regulated by oligochitosan treatment. Notably, 10 DEPs, including phosphatidylserine decarboxylase proenzyme and ATP-dependent molecular chaperone HSC82, were only detected in the control spores, whereas one DEP, a non-annotated predicted protein, was only detected in the oligochitosan-treated spores. CONCLUSION: Oligochitosan may affect the spore germination of B. cinerea by impairing protein function. These findings have practical implications with respect to the use of oligochitosan for controlling fungal pathogens. A, Potential applications and antifungal activities of engineered nanomaterials against gray J Sci Food Agric 2019; 99: 2622-2628

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‘Omics’ and Plant Responses to Botrytis cinerea

Cited by 72 publications

References 78 publications

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Microarray analysis of Arabidopsis WRKY33 mutants in response to the necrotrophic fungus Botrytis cinerea

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

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